Rapid diagnosis of phenylketonuria and other aminoacidemias by quantitative analysis of amino acids in neonatal blood spots by gas chromatography–mass spectrometry

Deng, Chunhui; Shang, Chunqing; Hu, Yuekai; Zhang, Xiangmin

doi:10.1016/s1570-0232(02)00283-0

Cited by 34 publications

(18 citation statements)

References 9 publications

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“…Sensitivities of 0.5839 and 0.2724 Hz/mgL −1 were obtained for the electrodes, respectively, indicating that the imprinted poly(AN-co-AA) electrode had almost twice the sensitivity. These sensitivities are lower than that reported for GC-MS (6.4 × 10 5 peak area/µM) [28], but better than that reported for a dehydrogenase based reagentless biosensor (0.32 × 10 −3 A/M) [2].…”

Section: Sensitivity Of the Sensorcontrasting

confidence: 62%

Application of a quartz crystal nanobalance to the molecularly imprinted recognition of phenylalanine in solution

Mirmohseni

Shojaei

Farbodi

2008

Biotechnol Bioproc E

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A quartz crystal nanobalance (QCN) biosensor was developed for the selective determination of phenylalanine (Phe) in aqueous solutions. A Phe imprinted copolymer was synthesized using polyacrylonitrile and acrylic acid [poly(AN-co-AA)]. The copolymer was then coated on quartz crystal electrode to form complementary structures for the template recognition of Phe. The composite electrode was then used to determine Phe levels in solution. Determinations were based on frequency shifts of molecularly imprinted polymer (MIP) modified quartz crystal electrode caused by Phe adsorption. The frequency shifts were linearly dependent on Phe concentration over the range 50~500 mgL −1 . The results obtained show that the imprinted poly(AN-co-AA) modified biosensor had higher sensitivity (0.5839 Hz/mgL −1 ) than a non-molecularly imprinted copolymer (0.2724 Hz/mgL −1 ). Furthermore, good reproducibility, R.S.D. = 1.84% (n = 7) was observed, and the detection limit was 45 mgL −1 . The selectivity of the imprinted poly(AN-co-AA) modified biosensor was examined using a number of analytes similar to Phe,=áKÉK, dopamine (DA), ascorbic acid (AscA), vanillylmandelic acid (VMA), uric acid (UA), tryptophan (Trp), and tyrosine (Tyr), and the results obtained showed a size dependent selective effect. © KSBB hÉóïçêÇëW=éÜÉåóä~ä~åáåÉI=ÄáçëÉåëçêI=èì~êíò=Åêóëí~ä=å~åçÄ~ä~åÅÉI=éçäóE^kJÅçJ^^FI=jfm= = = = =

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Section: Sensitivity Of the Sensorcontrasting

confidence: 62%

Application of a quartz crystal nanobalance to the molecularly imprinted recognition of phenylalanine in solution

Mirmohseni

Shojaei

Farbodi

2008

Biotechnol Bioproc E

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“…20,21 It can seen from Table 2 that the ratio of Phe to Tyr in PKU-positive blood samples was much higher than that in normal blood samples. Therefore, simultaneous screening for MSUD and PKU could be achieved by analysis of the five amino acids in neonatal blood samples using the proposed method.…”

Section: Determination Of Amino Acids In Neonatal Blood Spot Samplesmentioning

confidence: 95%

“…In our previous studies, we demonstrated the feasibility of diagnosis of PKU and MSUD using GC/MS analysis of amino acids in neonatal blood samples. 20,21 In the present method, isobutyl chloroformate was introduced for derivatization of amino acids in blood samples. Moreover, a simple and rapid sample SPME technique was developed for extraction of the amino acid derivatives.…”

Section: Detection Limit Recovery and Precisionmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14] Gas chromatography/mass spectrometry (GC/MS) has been shown to be a simple and high-speed technique with a high resolving power for the determination of amino acids in body fluids, and has been widely applied to neonatal screening. [15][16][17][18][19][20][21] It is well known that a preliminary derivatization of amino acids is required to make them volatile and thereby amenable to analysis by GC or GC/MS. Trifluoroacetic anhydride plus n-butanol has been used for derivatization of amino acids, and modifies both amino and carboxyl groups.…”

mentioning

confidence: 99%

“…Trifluoroacetic anhydride plus n-butanol has been used for derivatization of amino acids, and modifies both amino and carboxyl groups. 21,22 However, this procedure requires two steps including esterification and acylation, which are time-consuming and tedious. Recently, the silylation agents bis(trimethylsilyl)trifluoroacetamide (BSTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA) were developed for the derivatization of amino acids.…”

mentioning

confidence: 99%

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Rapid determination of amino acids in neonatal blood samples based on derivatization with isobutyl chloroformate followed by solid‐phase microextraction and gas chromatography/mass spectrometry

Deng

Zhang

2004

Rapid Comm Mass Spectrometry

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The purpose of this study was to develop a simple, rapid and sensitive analytical method for determination of amino acids in neonatal blood samples. The developed method involves the employment of derivatization and a solid-phase microextraction (SPME) technique together with gas chromatography/mass spectrometry (GC/MS). Amino acids in blood samples were derivatized by a mixture of isobutyl chloroformate, methanol and pyridine, and the N(O,S)-alkoxycarbonyl alkyl esters thus formed were headspace extracted by a SPME fiber. Finally, the extracted analytes on the fiber were desorbed and detected by GC/MS in electron impact (EI) mode. L-Valine, L-leucine, L-isoleucine, L-phenylanaline and L-tyrosine in blood samples were quantitatively analyzed by measurement of the corresponding N(O,S)-alkoxycarbonyl alkyl esters using an external standard method. SPME conditions were optimized, and the method was validated. The method was applied to diagnosis of neonatal phenylkenuria (PKU) and maple syrup urine disease (MSUD) by the analyses of five amino acids in blood samples. The results showed that the proposed method is a potentially powerful tool for simultaneous screening for neonatal PKU and MSUD.

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Derivatization Techniques in Dried Blood Spot Analysis

Ingels¹,

Sadones²,

Kesel³

et al. 2014

Dried Blood Spots

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Rapid diagnosis of phenylketonuria and other aminoacidemias by quantitative analysis of amino acids in neonatal blood spots by gas chromatography–mass spectrometry

Cited by 34 publications

References 9 publications

Application of a quartz crystal nanobalance to the molecularly imprinted recognition of phenylalanine in solution

Application of a quartz crystal nanobalance to the molecularly imprinted recognition of phenylalanine in solution

Rapid determination of amino acids in neonatal blood samples based on derivatization with isobutyl chloroformate followed by solid‐phase microextraction and gas chromatography/mass spectrometry

Derivatization Techniques in Dried Blood Spot Analysis

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