Rapid determination of cephalosporins with an immobilized enzyme reactor and sequential subtractive spectrophotometric detection in an automated flow-injection system

Decristoforo, Georg; Knauseder, Franz

doi:10.1016/s0003-2670(00)81495-6

Cited by 12 publications

(1 citation statement)

References 14 publications

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“…penicillinase Penicilloic acid þ H þ These reactions were monitored by measuring with pH electrodes [18][19][20][21][22] or ion selective field effect transistor-based biosensors [23]. Analyses of β-lactam antibiotics using a microbial receptor protein-based biosensor [24], optical monitoring employing an enzyme reactor/flow injection system-based biosensor [25], fiber optic-based biosensor [26], and surface plasmon resonance-based biosensor [27,28] on the inhibition of carboxypeptidase activity have also been reported. Hence, it is evident that the need for a simple, reliable, and specific determination method for the cephalosporin group of antibiotics has led to the development of many biological sensors.…”

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confidence: 99%

Cephalosporins Determination with a Novel Microbial Biosensor Based on Permeabilized Pseudomonas aeruginosa Whole Cells

Kumar

Kundu

Pakshirajan

et al. 2008

Appl Biochem Biotechnol

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A new potentiometric microbial biosensor based on Pseudomonas aeruginosa was developed in this study for detecting the cephalosporin group of antibiotics. Preliminary results with the biosensor indicated that P. aeruginosa cells, when treated with lysozyme, showed more efficiency in detecting cephalosporin C in a wide concentration range of 0.1-11 mM with high sensitivity compared to the normal cells. Optimization of the three important biosensor design parameters permeabilized cell contents, quantities of gelatin, and glutaraldehyde resulted in high performance of the biosensor. The optimized values of the above parameters were cell contents 2.5 mg/cm(2), gelatin 8.5 mg/cm(2), and 0.25% glutaraldehyde. The assay conditions, namely phosphate buffer pH, ionic strength, and temperature, were optimized for best performance of the biosensor. The specificity test of the biosensor towards detecting different beta-lactam antibiotics showed good response only for the cephalosporins. The operational and storage stability in detecting cephalosporin C indicated very good potential of the biosensor in detecting cephalosporins with high accuracy.

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confidence: 99%

Cephalosporins Determination with a Novel Microbial Biosensor Based on Permeabilized Pseudomonas aeruginosa Whole Cells

Kumar

Kundu

Pakshirajan

et al. 2008

Appl Biochem Biotechnol

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Electrochemical reduction and oxidation of two cephalosporin antibiotics: Ceftriaxone (rocephin) and cefoperazone (cefobid)

1993

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Two cephalosporin antibiotic derivatives, ceftriaxone (CFRX) and cefoperazone (CFPZ), have been studied by direct current, differential pulse polarography (DPP), and cyclic voltammetry. Both give rise to two irreversible two-electron reduction steps. The characteristics of the corresponding electrode reactions are discussed. The electrooxidation of both drugs was also studied at the carbon paste electrode (CPE). Both drugs give rise to one oxidation peak at about 1-1.05 V vs. SCE (pH 1.6, phosphate buffer solution). Optimum pH ranges for the determination of these drugs at the CPE are also given. Linear calibration plots were obtained over the concentration ranges 0.49 to 99 pg/mL and 0.66 to 534 pg/mL for CFRX and CFPZ, respectively. Detection limits of 2.5 ng/mL and 0.13 pg/mL for CFRX and CFPZ, respectively, were readily achieved.

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Stoffbeschreibung

Schwarze¹,

Lommer²,

Beyer³

et al. 1991

Hagers Handbuch Der Pharmazeutischen Praxis

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Rapid determination of cephalosporins with an immobilized enzyme reactor and sequential subtractive spectrophotometric detection in an automated flow-injection system

Cited by 12 publications

References 14 publications

Cephalosporins Determination with a Novel Microbial Biosensor Based on Permeabilized Pseudomonas aeruginosa Whole Cells

Cephalosporins Determination with a Novel Microbial Biosensor Based on Permeabilized Pseudomonas aeruginosa Whole Cells

Electrochemical reduction and oxidation of two cephalosporin antibiotics: Ceftriaxone (rocephin) and cefoperazone (cefobid)

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