A new, automated flow Injection analysis (FIA) method was developed for rapid and specific determination of /3-lactams like penicillins and cephalosporins. The enthalpies of enzymatic reactions are measured In continuous flowing streams by means of thermistors. Enzyme reactions are carried out on small solid bed reactor columns, whereby the selectivity for various analytes Is exclusively dependent on the specificity of the chosen enzyme system. Accuracy of enzyme thermistor flow Injection analysis (ET-FIA) was evaluted by comparison with HPLC. Statistically no significant difference was found between the two methods. The minimal amount of K-penlclllin V detectable by ET-FIA Is 10® g. Precision of the method was determined by 10 consecutive Injections of the same sample; the relative standard deviation Is ±0.8%.Linearity Is obtained In a concentration range between 0.1 mg/mL and 10 mg/mL for both penicillins and cephalosporins. Sample capacity Is 40-45 samples/h and time of analysis for one single sample Is less than 80 s.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.