Rapid detection of norovirus genogroup II in clinical and environmental samples using recombinase polymerase amplification

Han, Yanzhen; Wang, Jianchang; Zhang, Shuhong; Wang, Jinfeng; Qin, Chen; Han, Yanqing; Xu, Xiangdong

doi:10.1016/j.ab.2020.113834

Cited by 13 publications

(11 citation statements)

References 40 publications

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“…In recent years, several groups have developed detection methods for GII norovirus based exclusively on RPA, and all have shown relatively high sensitivity ( Moore and Jaykus, 2017 ; Ma et al, 2018 ; Han et al, 2020 ; Jia et al, 2020 ). Moore et al developed an RPA assay for GII.4 norovirus that can identify two GII.4 norovirus strains that have slight differences in target sequences ( Moore and Jaykus, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, it requires expensive and sophisticated thermal cyclers to complete the assay, which is not feasible for smaller, more basic laboratories. As alternatives, methods involving isothermal amplification technology have also been applied for the detection of norovirus, such as nuclear acid sequence-based amplification ( Patterson et al, 2006 ), loop-mediated isothermal amplification ( Luo et al, 2014 ), and recombinase polymerase amplification (RPA; Han et al, 2020 ; Jia et al, 2020 ). Unlike qPCR, isothermal amplification techniques do not require the use of sophisticated thermal cyclers, as in qPCR, which allows for simplification of the procedure and improved accessibility.…”

Section: Introductionmentioning

confidence: 99%

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Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection

et al. 2022

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Genogroup II genotype 4 (GII.4) norovirus causes acute gastroenteritis in children, and its infection is more severe than that of other genotypes. Early and precise detection and treatment are critical for controlling its spread and reducing the severity of infection. In this study, a rapid and efficient isothermal assay for the GII.4 norovirus detection (GII.4-CRISPR detection) was developed based on the CRISPR/Cas13a system. The assay can be applied without expensive instrumentation, and the results can be read via both fluorescence and lateral flow strip (LFS). The analytical sensitivity of this assay was 5 copies/reaction, and there was no cross-reaction with other genotypes of norovirus or other clinically common pathogens. There was a coincidence rate of 100% between our assay and commercial quantitative polymerase chain reaction. GII.4-CRISPR detection improves upon the shortcomings of some previously established molecular methods of detection, particularly with regard to accessibility. It provides an alternative tool for outbreak control and early diagnosis of GII.4 norovirus infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection

et al. 2022

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“…Han et al developed a real-time reverse transcription RPA (RT-RPA) for norovirus rapid and specific detection. The limit of detection was 1.66 × 10 2 copies/μL . Enterovirus 71 (EV71) was also detected with the RPA method, and the sensitivity was about 3.7 genome copies/reaction .…”

Section: Methods Of Foodborne Viruses Detectionmentioning

confidence: 99%

Detection Methods for Foodborne Viruses: Current State-of-Art and Future Perspectives

Yin

Zhang

Xiao

et al. 2023

J. Agric. Food Chem.

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Foodborne viruses have been recognized as important threats to food safety and human health. Rapid and accurate detection is one of the most crucial measures for food safety control. With the development of biology, chemistry, nanoscience, and related interdisciplines, detection strategies have been devised and advanced continuously. This review mainly focuses on the progress of detection methods for foodborne viruses. The current detection methods for foodborne viruses are summarized, including traditional electron microscopy and cultural isolation, immunoassay, molecular technology, biosensors, and newly emerging CRISPR/Cas-based detection technology. Furthermore, a comparison of the detection methods was objectively discussed. This review provides a comprehensive account of foodborne virus detection methods from fundamentals to state-of-the-art and illustrates the advantages and disadvantages of the current methods and proposes the future trends and directions for foodborne virus detection. It is hoped that this review can update current knowledge and present blueprints in order to accelerate futuristic development.

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“…One of the challenges with the designed primers given their length was the ability to more broadly react with genotypes other than GII.4, as the designed RT-RPA assay was reactive with another GII.4 strain (GII.4 Sydney), but only partially reactive with a GII.3 and not reactive with GI or a panel of other enteric viruses and bacteria. More recently, Han et al (2020) report an RT-RPA for all GII genogroup noroviruses (including GII.4, GII.P16-GII.2, and GII.P17-GII.17) with a limit of detection of 166 copies/µL. When testing real life samples, both the RT-RPA and RT-qPCR resulted in similar positive food samples (8/20) and stool samples (10/18).…”

Section: Two Bucket Syndrome: Caliciviridae Family Norovirusmentioning

confidence: 99%

Recent Developments in Isothermal Amplification Methods for the Detection of Foodborne Viruses

et al. 2022

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Foodborne and enteric viruses continue to impose a significant public health and economic burden globally. As many of these viruses are highly transmissible, the ability to detect them portably, sensitively, and rapidly is critical to reduce their spread. Although still considered a gold standard for detection of these viruses, real time polymerase chain reaction (PCR)-based technologies have limitations such as limited portability, need for extensive sample processing/extraction, and long time to result. In particular, the limitations related to the susceptibility of real time PCR methods to potential inhibitory substances present in food and environmental samples is a continuing challenge, as the need for extensive nucleic acid purification prior to their use compromises the portability and rapidity of such methods. Isothermal amplification methods have been the subject of much investigation for these viruses, as these techniques have been found to be comparable to or better than established PCR-based methods in portability, sensitivity, specificity, rapidity, and simplicity of sample processing. The purpose of this review is to survey and compare reports of these isothermal amplification methods developed for foodborne and enteric viruses, with a special focus on the performance of these methods in the presence of complex matrices.

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Rapid detection of norovirus genogroup II in clinical and environmental samples using recombinase polymerase amplification

Cited by 13 publications

References 40 publications

Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection

Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection

Detection Methods for Foodborne Viruses: Current State-of-Art and Future Perspectives

Recent Developments in Isothermal Amplification Methods for the Detection of Foodborne Viruses

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