Recent Developments in Isothermal Amplification Methods for the Detection of Foodborne Viruses

Abstract

Foodborne and enteric viruses continue to impose a significant public health and economic burden globally. As many of these viruses are highly transmissible, the ability to detect them portably, sensitively, and rapidly is critical to reduce their spread. Although still considered a gold standard for detection of these viruses, real time polymerase chain reaction (PCR)-based technologies have limitations such as limited portability, need for extensive sample processing/extraction, and long time to result. In particular, the limitations related to the susceptibility of real time PCR methods to potential inhibitory substances present in food and environmental samples is a continuing challenge, as the need for extensive nucleic acid purification prior to their use compromises the portability and rapidity of such methods. Isothermal amplification methods have been the subject of much investigation for these viruses, as these techniques have been found to be comparable to or better than established PCR-based methods in portability, sensitivity, specificity, rapidity, and simplicity of sample processing. The purpose of this review is to survey and compare reports of these isothermal amplification methods developed for foodborne and enteric viruses, with a special focus on the performance of these methods in the presence of complex matrices.

A comprehensive review on novel synthetic foods: Potential risk factors, detection strategies, and processing technologies

“…Isothermal amplification refers to a particular type of PCR that maintains a constant temperature throughout the reaction. They have numerous benefits, including high analytical sensitivity, rapid detection, and higher tolerance to inhibitors (Suther et al., 2022). Due to their lower energy demand, faster reaction time, and no need for specialized and expensive equipment, isothermal amplification is well‐suited for on‐site and real‐time detection.…”

Medical viruses: diagnostic techniques

“…With no cross-reactions with other chosen viruses, LAMP has been employed for the quick detection of a few DNA viruses in human specimens, including HSV-1, hAdV40, and hAdV41. According to several pieces of research, RT-LAMP is more sensitive than NASBA and conventional RT-PCR [ 69 ].…”

Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay

“…The RPA method combines isothermal recombinase-driven primer targeting of template with strand-displacement DNA synthesis, which can occur at room temperature with minimal sample pretreatment. 22 , 23 Thus far, RPA-based diagnostics have been developed for the detection of different pathogens, including viruses, 24 fungi, 25 bacteria, 26 and parasites. 27 In this study, we combined RPA and CRISPR-Cas12a and developed a rapid and ultrasensitive diagnosis system for mpox virus detection.…”