Rapid detection of carbapenemase genes by multiplex real-time PCR

Abstract

The new assay was able to detect the presence of six different carbapenemase gene types in a single 3 h PCR.

Rapid and Simultaneous Detection of bla KPC and bla NDM by Use of Multiplex Real-Time PCR

“…The assay provides considerable advantages over methods that employ melting curve analysis and double-stranded DNA binding dyes (22), including the presence of an internal control to reduce false negatives, the ability to detect both bla KPC and bla NDM in the same target, and removal of the inherent variation in melting curves associated with allelic variation in target genes. After initial capital investments, the average cost per reaction is about $0.90.…”

Evaluation of carbapenem-resistant Enterobacteriaceae in a tertiary-level reference hospital in Rio Grande do Sul, Brazil

“…The BC test is based on the same principle as CN, except that the bromothymol blue indicator is used 6 . To confirm the production of carbapenemases, PCR was performed to detect the presence of the bla KPC , bla OXA-48 , bla NDM , and bla GES genes 7 . The sensitivity and specificity of the different phenotypic methods were calculated considering PCR as the standard methodology 6 .…”

First Report of OXA-48 Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli in Japan from a Patient Returned from Southeast Asia

“…Therefore, clinical isolates suspected to be ESBL producers due to demonstrating consistent resistance to various cephalosporins and reduced susceptibility (MIC, 2-4 mg/ml) to carbapenems would worth being subjected to PCR analyses using PCR primer sets for detecting known carbapenemase genes after screening using modified Hodge test (12). Furthermore, even in cases in which the clinical isolates show susceptibility to broad-spectrum b-lactams as found in the piperacillintazobactam-resistant K. pneumoniae (Kp1 in Table 1), the resistance profile to piperacillin-tazobactam may be a good marker for detecting KPC-or OXA-typecarbapenemase producers in addition to the modified Hodge test.…”