2013
DOI: 10.1128/jcm.03316-12
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Rapid and Simultaneous Detection of bla KPC and bla NDM by Use of Multiplex Real-Time PCR

Abstract: The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting bla KPC and bla NDM , two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with bla KPC -and bla NDM -carrying clinical is… Show more

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Cited by 30 publications
(18 citation statements)
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“…The high level of accuracy of the Carba NP test in this study was consistent with previous reports (8,9,(12)(13)(14)(15). The achievement of almost comparable sensitivity with the Rosco test was due to enhancing the clarity of test interpretation with a modified approach to reading what were otherwise uninterpretable tests.…”
Section: Discussionsupporting
confidence: 80%
“…The high level of accuracy of the Carba NP test in this study was consistent with previous reports (8,9,(12)(13)(14)(15). The achievement of almost comparable sensitivity with the Rosco test was due to enhancing the clarity of test interpretation with a modified approach to reading what were otherwise uninterpretable tests.…”
Section: Discussionsupporting
confidence: 80%
“…False-negative Carba NP results were reported previously, e.g., for Providencia rettgeri, Providencia stuartii, or Proteus mirabilis, in particular for mucoid isolates and isolates with NDM enzymes (11,29,31). Negative results were linked to variations in protein extraction, to species-specific characteristics, or to the agar type and ion content used for cultivation of the isolates in question.…”
Section: Discussionmentioning
confidence: 91%
“…1). False-negative Carba NP results have also been described for mucoid colonies, e.g., of P. rettgeri, P. stuartii, or P. mirabilis isolates (29,31). Negative results were attributed to difficulties in protein extraction, species-specific traits, or the influence of the agar type and ion content of the Carba NP test (30,31,36).…”
Section: Discussionmentioning
confidence: 99%
“…In 2014, EUCAST published new guidelines for the detection of resistance mechanisms, including carbapenemases, in which a CDT is recommended for carbapenemase confirmation (14,22,25). Recently, Nordmann et al described a new inhibitor-based biochemical assay for carbapenemase detection, the Carba NP test, which has been published in two versions: the Carba NP-I assay provides a positive or negative result (carbapenemase detected/not detected), whereas the Carba NP-II test has been designed to also discriminate among carbapenemase classes A, B, and D (26)(27)(28)(29). Apart from the original publications, few studies have systematically evaluated the Carba NP-I test for Enterobacteriaceae, and both the originally published protocol and modified versions were used.…”
mentioning
confidence: 99%