First Report of OXA-48 Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli in Japan from a Patient Returned from Southeast Asia

Nagano, Noriyuki; Endoh, Yasunobu; Nagano, Yukiko; Toyama, Masami; Matsui, Mari; Shibayama, Keigo; Arakawa, Yoshichika

doi:10.7883/yoken.66.79

Cited by 28 publications

(17 citation statements)

References 11 publications

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“…Moreover, the present study identified that the majority of the strains had OXA-48 and OXA-10 which are comparable to the results of other studies [5,10,[32][33][34]. In a study performed in Korea reported the prevalence of OXA-10 was 13.1% in P. aeruginosa [33] while in another study the prevalence of OXA-10 was 0.6% suggesting the great variability of OXA in different countries.…”

Section: Discussionsupporting

confidence: 90%

Prevalence, Genotyping of Escherichia coli and Pseudomonas aeruginosa Clinical Isolates for Oxacillinase Resistance and Mapping Susceptibility Behaviour

Chaudhary¹

2014

J Microb Biochem Technol

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In the present study, multi-drug resistant isolates of Escherichia coli and Pseudomonas aeruginosa were obtained from different clinical specimens and were subjected to molecular typing to detect the genes encoding oxacillinases in these isolates. Subsequently, antibacterial activity of drugs was tested against selected clinical isolates. Two hundred forty six isolates including 98 of E. coli and 148 of P. aeruginosa were collected from clinical specimens of different centers across India. Out of 246, 123 isolates showed weak synergy for ceftazidime or cefepime and imipenem or clavulanate and were considered as oxacillinase producers. Polymerase chain reaction (PCR) was performed to identify the variants of oxacillinases genes. Our results show a great diversity of occurance of oxacillinase (OXA) genes among clinical isolates. OXA-48 and OXA-10 were more prevalent in both E. coli (32.6% OXA-48; 16.3% OXA-10) and P. aeruginosa (OXA-48 32.4%; 27.0%) as evident by PCR. The incidence of other OXA genes in E. coli and P. aeruginosa varied from 4.0 to 12.1%. Of the tested drugs, cefepime plus sulbactam was found to be the most efficacious antibacterial agents with 81.6 to 89.2% susceptibility. Cefepime plus tazobactam was second most active antibacterial agent with 46.9 to 56.7% susceptibility. Surprisingly, imipenem plus cilastatin showed susceptibility to less than 45% isolates.From the above results, it is evident that cefepime plus sulbactam has enhanced in vitro antibacterial activity compared to cefepime alone imipenem plus cilastatin and cefepime plus tazobactam combination in oxacillinases. One significant finding of this study was that cefepime was found to be effective only against the isolates caring OXA-1 and OXA-2 but was found to be resistant to OXA-10, OXA-23, OXA-24, OXA-48, OXA-51 and OXA-58 genes; whereas cefepime in combination with sulbactam was found to be effective against most of these OXA genes in comparison to tazobactam combination.

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Section: Discussionsupporting

confidence: 90%

Prevalence, Genotyping of Escherichia coli and Pseudomonas aeruginosa Clinical Isolates for Oxacillinase Resistance and Mapping Susceptibility Behaviour

Chaudhary¹

2014

J Microb Biochem Technol

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“…However, this apparent discrepancy may be explained by the close cultural connections between South America, Portugal, and Spain. The first bla OXA-48 gene was de- (144) and Japan (145) were published in 2013, and it seems likely that this group of enzymes will successfully spread globally in the near future.…”

Section: Geographical Disseminationmentioning

confidence: 99%

OXA β-Lactamases

2014

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SUMMARY The OXA β-lactamases were among the earliest β-lactamases detected; however, these molecular class D β-lactamases were originally relatively rare and always plasmid mediated. They had a substrate profile limited to the penicillins, but some became able to confer resistance to cephalosporins. From the 1980s onwards, isolates of Acinetobacter baumannii that were resistant to the carbapenems emerged, manifested by plasmid-encoded β-lactamases (OXA-23, OXA-40, and OXA-58) categorized as OXA enzymes because of their sequence similarity to earlier OXA β-lactamases. It was soon found that every A. baumannii strain possessed a chromosomally encoded OXA β-lactamase (OXA-51-like), some of which could confer resistance to carbapenems when the genetic environment around the gene promoted its expression. Similarly, Acinetobacter species closely related to A. baumannii also possessed their own chromosomally encoded OXA β-lactamases; some could be transferred to A. baumannii , and they formed the basis of transferable carbapenem resistance in this species. In some cases, the carbapenem-resistant OXA β-lactamases (OXA-48) have migrated into the Enterobacteriaceae and are becoming a significant cause of carbapenem resistance. The emergence of OXA enzymes that can confer resistance to carbapenems, particularly in A. baumannii , has transformed these β-lactamases from a minor hindrance into a major problem set to demote the clinical efficacy of the carbapenems.

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“…Furthermore, the isolation of OXA-48-producing organisms from patients transferred from foreign countries to their native countries has also been increasingly reported (8,9). The first case of OXA-48 carbapenemase-producing Klebsiella pneumoniae and Escherichia coli was reported in Japan in December 2012 (9). Here we report the second case of an OXA-48-producing K. pneumoniae isolate from a clinical sample obtained from a patient returning to Japan.…”

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confidence: 79%

“…The first involved a man who had been hospitalized in a Southeast Asian country. Multidrug-resistant (resistant to third and fourth generation cephalosporins, aminoglycosides, and quinolones; MIC of imipenem was 2 mg/L) K. pneumoniae and E. coli were isolated from the sputum and/or feces, and PCR analyses of the carbapenemase genes revealed the presence of a bla OXA-48 -like gene in these isolates (9). Our patient had traveled to Turkey and received medical care at an ICU in Egypt.…”

mentioning

confidence: 89%

“…Since then, outbreaks with enormous clinical impact have been reported worldwide (4-7). Furthermore, the isolation of OXA-48-producing organisms from patients transferred from foreign countries to their native countries has also been increasingly reported (8,9). The first case of OXA-48 carbapenemase-producing Klebsiella pneumoniae and Escherichia coli was reported in Japan in December 2012 (9).…”

mentioning

confidence: 99%

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Isolation of OXA-48 Carbapenemase-Producing Klebsiella pneumoniae ST101 from an Overseas Traveler Returning to Japan

et al. 2014

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First Report of OXA-48 Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli in Japan from a Patient Returned from Southeast Asia

Cited by 28 publications

References 11 publications

Prevalence, Genotyping of Escherichia coli and Pseudomonas aeruginosa Clinical Isolates for Oxacillinase Resistance and Mapping Susceptibility Behaviour

Prevalence, Genotyping of Escherichia coli and Pseudomonas aeruginosa Clinical Isolates for Oxacillinase Resistance and Mapping Susceptibility Behaviour

OXA β-Lactamases

Isolation of OXA-48 Carbapenemase-Producing Klebsiella pneumoniae ST101 from an Overseas Traveler Returning to Japan

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