1983
DOI: 10.1073/pnas.80.9.2632
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Rapid degradation of unassembled ribulose 1,5-bisphosphate carboxylase small subunits in chloroplasts

Abstract: We have detected a proteolytic mechanism in chloroplasts that selectively and rapidly degrades the imported small subunit of ribulose 1,5-bisphosphate carboxylase when pools of the chloroplast-synthesized large subunit are depleted. This degradation system is constitutively present and appears to be responsible for precise stoichiometric accumulation of the two subunits of the enzyme. We believe similar proteolytic mechanisms participate in regulating the accumulation of other photosynthetic proteins during ch… Show more

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Cited by 226 publications
(124 citation statements)
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“…In vivo labeling of membrane proteins in the presence of cycloheximide, SDS-PAGE, and fluorography were performed as described previously (Schmidt and Mishkind, 1983) except 32SOi-was used at 0.5 mCi/mL. Labeling was terminated and cells were fractionated into membrane and soluble components as described by Jensen et al (1986).…”
Section: In Vivo Protein Labeling and Thylakoid Membrane Lsolationmentioning
confidence: 99%
“…In vivo labeling of membrane proteins in the presence of cycloheximide, SDS-PAGE, and fluorography were performed as described previously (Schmidt and Mishkind, 1983) except 32SOi-was used at 0.5 mCi/mL. Labeling was terminated and cells were fractionated into membrane and soluble components as described by Jensen et al (1986).…”
Section: In Vivo Protein Labeling and Thylakoid Membrane Lsolationmentioning
confidence: 99%
“…Schmidt and Mishkind (27) Figure 2. The extraction of CF, from broken cells was quantitative and independent of Chl concentration during the extraction (0.5-2.0 mg Chl/ml) and ofthe volume of material extracted (0.5-3.0 ml).…”
mentioning
confidence: 99%
“…However, it could as well be a consequence of overall reduced metabolic activity in the chloroplasts, some sort of feedback on transcription or some change in rates of degradation of these mRNAs. The best documented case of instability of chloroplast gene products in the absence of nuclear gene products is that of RuBP carboxylase as studied by Schmidt and Mishkind (27).…”
Section: Discussionmentioning
confidence: 99%
“…A, Chloroplast DNA (1I ig) prepared from wild-type maize (7ea mays FR 9Cms x FR 37) was digested with BamHI (B) or EcoRl (E), subjected to agarose gel (0.9%) electrophoresis with Tris-acetate buffer (19), and transferred to nitrocellulose filters (BA85, Schleicher & Schuell) after treating the gel with 0.5 N NaOH and 1.5 M NaCI and subsequently with 0.5 M Tris-HCI (pH 7.0) and 1.5 M NaCl (27). The nitrocellulose filter was prehybridized with 20 ,Ag (lanes 5 and 6) or 80 Ag (lanes 7 and 8) of hcf*-38 RNA for each lane as indicated in the figure.…”
Section: Methodsmentioning
confidence: 99%