1984
DOI: 10.1104/pp.75.3.781
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Synthesis and Turnover of the Chloroplast Coupling Factor 1 in Chlamydomonas reinhardi

Abstract: Studies on the biosynthesis of the chloroplast coupling factor 1 (CFI) in Cklamydemonas reixhardi have been initiated. The ratio of CF, to chlorophyll in the cell was shown to be independent of the density of the culture. No turnover of assembled CF1 could be detected, thus suggesting that CF1 was synthesized at a rate equivalent to that of net chlorophyll synthesis. A lag of between 5 to 7 minutes in the incorporation of radioactive precursor sulfate into assembled CF1 was measureable. This puts an upper limi… Show more

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Cited by 20 publications
(13 citation statements)
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“…This drastic reduction in lifetime is even more remarkable when one considers the minimal nature of the truncation; OE23-1A, for instance, is missing only 10 amino acids from its carboxyl terminus. Chloroplasts are known to contain proteases that quickly degrade subunits that are not immediately assembled (Bennett, 1981;Schmidt and Mishkind, 1983;Merchant and Selman, 1984), and some of these function in the thylakoid lumen (Merchant and Bogorad, 1986;Kuwabara and Suzuki, 1994;Li and Merchant, 1995). Although this appears to be a general mechanism for the regulation of subunit stoichiometry in protein complexes (Luzikov, 1986), the subunits of the OEC do not follow this pattern of assembly or degradation (Ettinger and Theg, 1991).…”
Section: Discussionmentioning
confidence: 99%
“…This drastic reduction in lifetime is even more remarkable when one considers the minimal nature of the truncation; OE23-1A, for instance, is missing only 10 amino acids from its carboxyl terminus. Chloroplasts are known to contain proteases that quickly degrade subunits that are not immediately assembled (Bennett, 1981;Schmidt and Mishkind, 1983;Merchant and Selman, 1984), and some of these function in the thylakoid lumen (Merchant and Bogorad, 1986;Kuwabara and Suzuki, 1994;Li and Merchant, 1995). Although this appears to be a general mechanism for the regulation of subunit stoichiometry in protein complexes (Luzikov, 1986), the subunits of the OEC do not follow this pattern of assembly or degradation (Ettinger and Theg, 1991).…”
Section: Discussionmentioning
confidence: 99%
“…The constituent polypeptides of the thylakoid ATPase do not generally accumulate as unassembled proteins (Merchant and Selman, 1984). Consequently, their steady-state levels are determined by the synthesis, maturation, or as- Proteins from whole cells (WC) or chloroplasts (Cp) isolated as described in "Materials and Methods" were denatured in sample buffer and fractionated in a 15% SDSpolyacrylamide gel, transferred to nitrocellulose, and reacted with antiserum raised against BRP or Oee2, the 23-kD polypeptide of the oxygen-evolving complex, which is localized in the chloroplast lumen.…”
Section: Accumulation Of the Chloroplast Atpasementioning
confidence: 99%
“…If CF1 of wild-type maize does not turn over significantly, as has been shown in Chlamydomonas (22), the observations that 12 d-old hcf*-38 seedlings contain 10% of normal levels of the a and ,8 subunits and that the a and # polypeptides are synthesized at about 60% of the wild-type rate, gives half-lives for the a and 3 subunits in hcjf*-38 of perhaps 12 h.…”
Section: Discussionmentioning
confidence: 81%