Rapid conversion and reversible conjugation of glutathione detoxification of microcystins in bighead carp (Aristichthys nobilis)

Li, Wei; Xie, Ping; He, Jun; Guo, Xiaochun; Tuo, Xun; Zhang, Wei; Wu, Laiyan

doi:10.1016/j.aquatox.2013.12.001

Cited by 34 publications

(31 citation statements)

References 40 publications

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“…Anders (1980) reported that S-substituted glutathione derivatives degraded very rapidly (t 1/2 ≈3.5 s) in microperfusion studies in rat kidneys. Meanwhile, in an MC-GSH exposure study, Li et al (2014b) also showed that any MC-RR-Cys detected in bighead carp in vivo was derived from MC-RR-GSH with a 96.7-fold increase of contents in kidney from 0.25 to 0.5 h postinjection. All above results demonstrated that MC-GSH could act as reactive intermediates and could be effectively converted to MC-Cys in both aquatic animals and mammals.…”

Section: Fig 2 Esi Lc/ms/ms Analysis Of Mc-lr and Its Two Metabolitementioning

confidence: 93%

“…Glutathione (GSH), the major non-protein thiol abundant in the cells of most organisms, was demonstrated to play an important role in the detoxification of MCs in both mammals and aquatic organisms (Kondo et al 1996;Pflugmacher et al 1998Pflugmacher et al , 2001He et al 2012;Li et al 2014b). The glutathione and cysteine conjugates of MCs (MC-GSH and MC-Cys) were firstly synthesized under chemical conditions by Kondo et al (1992) with Frit-FAB liquid chromatographymass spectrometry (LC/MS), and then the resulting conjugates were identified as two metabolites of MCs in vivo in the livers of mouse and rat.…”

Section: Introductionmentioning

confidence: 99%

“…However, the aforementioned studies merely focused on qualitative analyses. In recent years, some analytical methods were established for the quantitative determination of MCs and their metabolites in fish tissues (Dai et al 2008;Wu et al 2010), and on these bases, several investigations on detoxification of MCs in aquatic animals were performed under both field and laboratory conditions (Zhang et al 2009He et al 2012;Li et al 2014b).…”

Section: Introductionmentioning

confidence: 99%

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Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats

Guo

Chen

et al. 2015

Environ Sci Pollut Res

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Glutathione (GSH) plays crucial roles in antioxidant defense and detoxification metabolism of microcystin-LR (MC-LR). However, the detoxification process of MC-LR in mammals remains largely unknown. This paper, for the first time, quantitatively analyzes MC-LR and its GSH pathway metabolites (MC-LR-GSH and MC-LR-Cys) in the liver of Sprague-Dawley (SD) rat after MC-LR exposure. Rats received intraperitoneal (i.p.) injection of 0.25 and 0.5 lethal dose 50 (LD 50 ) of MC-LR with or without pretreatment of buthionine-(S,R)-sulfoximine (BSO), an inhibitor of GSH synthesis. The contents of MC-LR-GSH were relatively low during the experiment; however, the ratio of MC-LR-Cys to MC-LR reached as high as 6.65 in 0.5 LD 50 group. These results demonstrated that MC-LR-GSH could be converted to MC-LR-Cys efficiently, and this metabolic rule was in agreement with the data of aquatic animals previously reported. MC-LR contents were much higher in BSO+MC-LRtreated groups than in the single MC-LR-treated groups. Moreover, the ratio of MC-LR-Cys to MC-LR decreased significantly after BSO pretreatment, suggesting that the depletion of GSH induced by BSO reduced the detoxification of MCs. Moreover, MC-LR remarkably induced liver damage, and the effects were more pronounced in BSO pretreatment groups. In conclusion, this study verifies the role of GSH in the detoxification of MC-LR and furthers our understanding of the biochemical mechanism for SD rats to counteract toxic cyanobacteria.

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Section: Fig 2 Esi Lc/ms/ms Analysis Of Mc-lr and Its Two Metabolitementioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats

Guo

Chen

et al. 2015

Environ Sci Pollut Res

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“…The presence of MC-RR in bighead carp exposed to MC-RR-GSH suggests that MC-RR can be dissociated from MC-conjugates and MC conjugation with the thiol of GSH/Cys was found to be a reversible process in vivo. This result of reversible conjugation brought new insight on the GSH detoxification pathway of MCs which was detailed discussed in our recently published work [27]. MC-RR-GSH, MC-RR-Cys and MC-RR were also distributed in bile samples (Fig.…”

Section: Tablementioning

confidence: 52%

“…These samples were collected from the experiment of Li et al [27]. Briefly, the carp were divided into two groups at random, and fasted for 24 h before the initiation of the experiment.…”

Section: Applicationmentioning

confidence: 99%

Quantitative liquid chromatography–tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile

Xie

et al. 2014

Journal of Chromatography B

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Voltammetric Separation and Determination of Glutathione and L‐tyrosine with Chlorogenic Acid as an Electrocatalytic Mediator

Zhang

Chen

et al. 2017

Electroanalysis

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A novel and sensitive voltammetric method was proposed for separation and determination of glutathione (GSH) and L‐tyrosine (Tyr) at acetylene black and chitosan modified glassy carbon electrode (AB‐CS/GCE). By introducing chlorogenic acid (CGA) as a new electrocatalytic mediator, GSH could be detected at much lower potential with symmetric peak shape. Acetylene black and chitosan composite served as current signal amplifier for sensitive detection. The electrochemical behavior of GSH and Tyr in the presence of CGA was studied at AB‐CS/GCE and complete separation of anodic peaks was achieved. Under the optimum conditions, the electrocatalytic oxidation peak current of GSH showed a linear dependence on its concentration in the ranges of 2.0×10−7‐4.0×10−5 M with the detection limit of 5.8×10−8 M (S/N=3), while the oxidation peak current of Tyr was linear to its concentration from 2.5×10−6 to 4.3×10−4 M with the detection limit of 9.2×10−7 M (S/N=3) by differential pulse voltammetry (DPV). The established method has been applied to the simultaneous determination of GSH and Tyr in human urine with satisfactory results.

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Rapid conversion and reversible conjugation of glutathione detoxification of microcystins in bighead carp (Aristichthys nobilis)

Cited by 34 publications

References 40 publications

Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats

Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats

Quantitative liquid chromatography–tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile

Voltammetric Separation and Determination of Glutathione and L‐tyrosine with Chlorogenic Acid as an Electrocatalytic Mediator

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