Quantitative liquid chromatography–tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile

Li, Wei; Xie, Ping; He, Jun; Guo, Xiaochun; Yu, Dan; Chen, Liang

doi:10.1016/j.jchromb.2014.05.057

Cited by 17 publications

(9 citation statements)

References 31 publications

(36 reference statements)

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“…Otra posible limitación de los inmunoensayos se debe a que, además de las variantes de MCs presentes, podrían detectar metabolitos de detoxificación (conjugados MCs con cisteína y glutatión), que presentan mucho menor toxicidad que las correspondientes toxinas libres (Schmidt, et al, 2014;Li, et al, 2014). Para confirmar y cuantificar la presencia de diferentes variantes de MCs y distinguirlas de sus metabolitos, se utilizó un nuevo método ultrasensible de MALDI-TOF cuantitativo, originalmente validado para aguas y sueros de animales (Pírez-Schirmer, et al, 2019).…”

Section: Discussionunclassified

“…Se analizaron dos submuestras de cada acuario por triplicado. Este conjugado con cisteína representa un metabolito de detoxificación en animales, de menor toxicidad que la toxina libre (Schmidt, et al, 2014;Li, et al, 2014). En la Tabla 4 se reportan los resultados de concentración de MC-LR obtenidos por MALDI-TOF cuantitativo.…”

Section: Concentración De Microcistinas En Australoheros Facetusunclassified

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Nuevas metodologías para el análisis de microcistinas en peces. Estudio de Astraloheros Facetus expuestos in vitro.

Baharian

Letamendía

Pírez

et al. 2020

INNOTEC

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Section: Discussionunclassified

Section: Concentración De Microcistinas En Australoheros Facetusunclassified

Nuevas metodologías para el análisis de microcistinas en peces. Estudio de Astraloheros Facetus expuestos in vitro.

Baharian

Letamendía

Pírez

et al. 2020

INNOTEC

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“…MCs are transported to the intestine and further are excreted by feces. The other route is absorption and subsequent conjugation in the liver and rapid excretion of this compound in the bile [54]. MC-LR and its derivatives (MC-LR-GSH and MC-LR-Cys) may also enter the enterohepatic circulation and, being reabsorbed into the blood stream, may reach the brain, heart, lungs, and even testicles [55].…”

Section: Cyclic Peptides-toxicity and Biotransformationsmentioning

confidence: 99%

Mechanisms of Cyanotoxin Toxicity—Carcinogenicity, Anticancer Potential, and Clinical Toxicology

Vankova¹,

Pasheva²,

Kiselova-Kaneva³

et al. 2021

Medical Toxicology

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Cyanoprokaryotes are distributed worldwide and they produce various bioactive compounds, including cyanotoxins. The major route of human exposure to cyanotoxins is the oral intake by using contaminated drinking water, by incidental intake of contaminated water during recreational and professional activities, and by consuming contaminated food or dietary supplements prepared from cyanobacteria. The prolonged chronic exposure to low concentrations of cyanotoxins provokes cell damage and may increase the risk for cancer development. Due to the variety of cyanotoxin chemical structures, different mechanisms of their toxic effects are known. At the same time, some of the natural compounds produced by cyanoprokaryotes have anticancer potential and are promising sources for the development of novel drugs. This chapter is dedicated to the target mechanisms behind the effects of the widely distributed cyanotoxins with an impact on human health, microcystins, nodularins, and cylindrospermopsin.

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“…Guo et al [54] have used LC-MS for the analysis ported. [63] However, Wu et al [55] have quantified MC-RR and its metabolites through mixed-mode cation-exchange SPE, then quantifying them through liquid chromatography electrospray ionization ion trap mass spectrometry (LC-ESI-ITMS) in the fish liver.…”

Section: Mc-htyr Mc-lr Mc-hilr Mc-wr Mc-la Mc-ly Mc-lw Andmentioning

confidence: 99%

Recent developments in the methods of quantitative analysis of microcystins

Kumar

Rautela

Kesari

et al. 2020

J Biochem & Molecular Tox

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Cyanotoxins are produced by the toxic cyanobacterial species present in algal blooms formed in water bodies due to nutrient over-enrichment by human influences and natural environmental conditions. Extensive studies are available on the most widely encountered cyanotoxins, microcystins (MCs) in fresh and brackish water bodies. MC contaminated water poses severe risks to human health, environmental sustainability, and aquatic life. Therefore, commonly occurring MCs should be monitored. Occasionally, detection and quantification of these toxins are difficult due to the unavailability of pure standards. Enzymatic, immunological assays, and analytical techniques like protein phosphatase inhibition assay, enzymelinked immunosorbent assay, high-performance liquid chromatography, liquid chromatography-mass spectrometry, and biosensors are used for their detection and quantification. There is no single method for the detection of all the different types of MCs; therefore, various techniques are often combined to yield reliable results. Biosensor development offered a problem-solving approach in the detection of MCs due to their high accuracy, sensitivity, rapid response, and portability. In this review, an endeavor has been made to uncover emerging techniques used for the detection and quantification of the MCs.

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Quantitative liquid chromatography–tandem mass spectrometry method for determination of microcystin-RR and its glutathione and cysteine conjugates in fish plasma and bile

Cited by 17 publications

References 31 publications

Nuevas metodologías para el análisis de microcistinas en peces. Estudio de Astraloheros Facetus expuestos in vitro.

Nuevas metodologías para el análisis de microcistinas en peces. Estudio de Astraloheros Facetus expuestos in vitro.

Mechanisms of Cyanotoxin Toxicity—Carcinogenicity, Anticancer Potential, and Clinical Toxicology

Recent developments in the methods of quantitative analysis of microcystins

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