Rapid communication: physical and genetic mapping of the Peroxisome Proliferator Activated Receptor gamma (PPAR-gamma) gene to porcine chromosome 13.

Grindflek, Eli; Sundvold, Hilde; Lien, Sigbjørn; Rothschild, Max F.

doi:10.2527/2000.7851391x

Cited by 30 publications

(20 citation statements)

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“…However, no significant difference was observed for PPAR-γ activity after low-zinc intake (0.5-1.5 mg/kg) in mice fed with a high-fat diet [20]. PPAR-γ is a member of the nuclear receptor superfamily and regulates the expression of several genes encoding proteins involved in adipocyte differentiation, fatty acid storage and glucose metabolism [21]. Downregulation PPAR-γ plays an important role in the pathology of numerous diseases such as diabetes, atherosclerosis, and cancer [22].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: The Effect of Zinc Supplementation on Expressed Levels of Peroxisome Proliferator-Activated Receptor Gamma and Glucose Transporter Type 1 Genes in Newborns of Women with Gestational Diabetes Mellitus

Heidarzadeh

Samimi

Seifati

et al. 2016

Biol Trace Elem Res

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The current study was designed to determine the beneficial effects of zinc supplementation on expressed levels of peroxisome proliferator-activated receptor gamma (PPAR-γ) and glucose transporter type 1 (GLUT1) genes in newborns of women with gestational diabetes mellitus (GDM). This randomized, double-blind, placebo-controlled clinical trial was performed among 40 women with GDM. Patients were randomly allocated to intake either 233 mg zinc gluconate (containing 30 mg zinc) (n = 20) or a placebo (n = 20) for 6 weeks.PPAR-γ and GLUT1 mRNA levels were quantified in umbilical cord blood of newborns of women with GDM. After 6 weeks of intervention, the change in serum zinc levels was greater in women consuming zinc than in the placebo group (+11.1 ± 13.4 vs. −4.8 ± 17.3 mg/dL, P = 0.002). Quantitative results of RT-PCR demonstrated that compared with the placebo, zinc supplementation resulted in a significant increase of expressed levels of PPAR-γ mRNA (P < 0.001) and GLUT1 mRNA (P < 0.001) in umbilical cord blood of newborns of women with GDM. Taken together, the current study demonstrated that zinc supplementation for 6 weeks among GDM women increased the mRNA levels of PPAR-γ and GLUT1 in their newborns compared with the placebo group.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: The Effect of Zinc Supplementation on Expressed Levels of Peroxisome Proliferator-Activated Receptor Gamma and Glucose Transporter Type 1 Genes in Newborns of Women with Gestational Diabetes Mellitus

Heidarzadeh

Samimi

Seifati

et al. 2016

Biol Trace Elem Res

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“…H-FABP is involved in intracellular targeting of fatty acids and facilitates the transport of fatty acids from the membrane to the sites of fatty acid oxidation or esterification into TAG or phospholipids (Krag et al, 2007). PPARg is a member of the nuclear receptor superfamily and regulates the expression of several genes encoding proteins involved in adipocyte differentiation and fat deposition (Grindflek et al, 2000). It has been reported that PPARg promotes gene expression of FABP and suppresses the HSL gene expression (Grindflek et al, 2000).…”

Section: Discussionmentioning

confidence: 98%

“…PPARg is a member of the nuclear receptor superfamily and regulates the expression of several genes encoding proteins involved in adipocyte differentiation and fat deposition (Grindflek et al, 2000). It has been reported that PPARg promotes gene expression of FABP and suppresses the HSL gene expression (Grindflek et al, 2000). However, few studies examined the effects of dietary protein levels on PPARg gene expression in porcine muscle tissue.…”

Section: Discussionmentioning

confidence: 99%

Low protein diet up-regulate intramuscular lipogenic gene expression and down-regulate lipolytic gene expression in growth–finishing pigs

et al. 2012

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“…PPAR γ is a member of the nuclear receptor superfamily and regulates the expression of several genes encoding proteins involved in adipocyte differentiation and fat deposition [17]. It has been reported to promote LPL and FABP gene expression and to suppress the HSL gene [17].…”

Section: Discussionmentioning

confidence: 99%

Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue

et al. 2010

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BackgroundHigh dietary protein can reduce fat deposition in animal subcutaneous adipose tissue, but little is known about the mechanism.MethodsSixty Wujin pigs of about 15 kg weight were fed either high protein (HP: 18%) or low protein (LP: 14%) diets, and slaughtered at body weights of 30, 60 or 100 kg. Bloods were collected to measure serum parameters. Subcutaneous adipose tissues were sampled for determination of adipocyte size, protein content, lipid metabolism-related gene expression, and enzyme activities.ResultsHP significantly reduced adipocyte size, fat meat percentage and backfat thickness, but significantly increased daily gain, lean meat percentage and loin eye area at 60 and 100 kg. Serum free fatty acid and triglyceride concentrations in the HP group were significantly higher than in the LP group. Serum glucose and insulin concentrations were not significantly affected by dietary protein at any body weight. HP significantly reduced gene expression of acetyl CoA carboxylase (ACC), fatty acid synthase (FAS) and sterol regulatory element binding protein 1c (SREBP-1c) at 60 kg and 100 kg; however, the mRNA level and enzyme activity of FAS were increased at 30 kg. HP promoted gene and protein expression and enzyme activities of lipoprotein lipase (LPL), carmitine palmtoyltransferase-1B (CPT-1B), peroxisome proliferator-activated receptor γ (PPARγ) and adipocyte-fatty acid binding proteins (A-FABP) at 60 kg, but reduced their expression at 100 kg.Gene expression and enzyme activity of hormone sensitive lipase (HSL) was reduced markedly at 60 kg but increased at 100 kg by the high dietary protein. Levels of mRNA, enzyme activities and protein expression of ACC, FAS, SREBP-1c and PPARγ in both LP and HP groups increased with increasing body weight. However, gene and protein expression levels/enzyme activities of LPL, CPT-1B, A-FABP and HSL in both groups were higher at 60 kg than at 30 and 100 kg.ConclusionFat deposition in Wujin pigs fed high dietary protein for 25 weeks was reduced mainly by depression of lipogenic gene expression. The mechanism of lipid transport, lipolysis and oxidation in adipose tissue regulated by dietary protein appeared to be different at 60 kg and 100 kg body weights.

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Rapid communication: physical and genetic mapping of the Peroxisome Proliferator Activated Receptor gamma (PPAR-gamma) gene to porcine chromosome 13.

Cited by 30 publications

References 0 publications

RETRACTED ARTICLE: The Effect of Zinc Supplementation on Expressed Levels of Peroxisome Proliferator-Activated Receptor Gamma and Glucose Transporter Type 1 Genes in Newborns of Women with Gestational Diabetes Mellitus

RETRACTED ARTICLE: The Effect of Zinc Supplementation on Expressed Levels of Peroxisome Proliferator-Activated Receptor Gamma and Glucose Transporter Type 1 Genes in Newborns of Women with Gestational Diabetes Mellitus

Low protein diet up-regulate intramuscular lipogenic gene expression and down-regulate lipolytic gene expression in growth–finishing pigs

Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue

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