Low protein diet up-regulate intramuscular lipogenic gene expression and down-regulate lipolytic gene expression in growth–finishing pigs

Wang, J.; Zhao, Sheng‐Yin; Song, Xinlei; Pan, Hongbin; Li, W.Z.; Zhang, Y.Y.; Shi-zheng, Gao; Chen, D.W.

doi:10.1016/j.livsci.2012.05.018

Cited by 20 publications

(20 citation statements)

References 43 publications

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“…; Wang et al . ). But our observations were that in all the three treatment groups IMF contents were greater than the control group, and the highest IMF content was seen in the 19% protein group.…”

Section: Discussionmentioning

confidence: 97%

“…In the experiment by Wang et al . (), the pigs were organized in two levels of dietary protein, which was formulated at low (LP: 14%) and high (LP: 18%) levels. But, in this study early‐weaned female yaks in the control group were grazed without dietary protein supplementation; the other three dietary protein levels (supplementary) were low protein (LP: 17%), medium protein (MP: 19%) and high protein (HP: 21%), respectively.…”

Section: Discussionmentioning

confidence: 99%

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Effects of different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early‐weaned yaks

Zhang

Wang

Peng

et al. 2014

Animal Science Journal

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This study was conducted to estimate different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early-weaned yaks. Results showed that supplementary dietary protein significantly increased final weight, average daily gain (ADG), intramuscular fat (IMF), serum free fatty acid (FFA), total triglycerides, total cholesterol (Ch), low-density lipoprotein cholesterol (LDL) and high-density lipoprotein cholesterol (HDL) content. There was a quadratic response of ADG, IMF, FFA, Ch, HDL and LDL to dietary crude protein (CP) level. Lipoprotein lipase (LPL), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) enzyme activities were significantly increased by supplementary dietary CP, while hormone-sensitive lipase (HSL) and carnitine palmitoyltransferase-1 (CPT-1) activities were significantly decreased. LPL, ACC and FAS enzyme activities showed quadratic increase as dietary CP increased. Peroxisome proliferator-activated receptor γ (PPARγ), LPL, FAS, sterol regulatory element binding protein 1 (SREBP-1), ACC, stearoyl-CoA desaturase (SCD) and heart fatty-acid binding protein (H-FABP) gene expression were significantly increased by supplementary dietary CP, while HSL and CPT-1 gene expression were significantly decreased. PPARγ, LPL, SREBP-1, ACC and H-FABP gene expression showed quadratic increase as dietary CP increased. These results indicated that supplementary dietary protein increased IMF accumulation mainly to increased intramuscular lipogenic gene expression and decreased lipolytic gene expression.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Effects of different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early‐weaned yaks

Zhang

Wang

Peng

et al. 2014

Animal Science Journal

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“…Our previous study (Zhao et al., ; Wang et al., ) had assessed the effects of high protein diet on subcutaneous and muscle fat deposition of Wujin pigs. However, relatively little is known about how the HP diet influences the protein accretion in muscle tissue.…”

Section: Discussionmentioning

confidence: 99%

“…() and Wang et al. () assessed the effects of higher dietary protein level on porcine subcutaneous and muscle fat deposition by measuring the expression of lipid metabolism‐related genes. The objective of the present study was to investigate the influence of HP diet on the expression of genes related to translation initiation in muscle tissue of Wujin pigs at different growth stages and investigate the underlying mechanism of skeletal muscle protein accumulation regulated by dietary protein.…”

Section: Introductionmentioning

confidence: 99%

The effect of dietary protein levels on the expression of genes coding for four selected protein translation initiation factors in muscle tissue of Wujin pig

Liu

Wang

Huang

et al. 2013

Animal Physiology Nutrition

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The objective of this study was to investigate the regulatory mechanism underlying the increased muscle protein accumulation in pigs while were fed a high protein diet. The eukaryotic initiation factors (eIFs) have been reported to involve in muscle protein synthesis. We investigated the mRNA and protein expression levels of eIF2B1, 4A1, 4B and 4E in Wujin pigs fed either a high protein (HP: 18%) or a low protein (LP: 14%) diet at 30, 60 or 100 kg body weight, based on real-time PCR and western blotting analyses. Our results indicated that the expression levels of eIF2B1 mRNA and protein were increased by HP diet at all body weight. The HP diet showed higher mRNA and protein levels of eIF4B gene at 60 and 100 kg. The protein expression of eIF4E phosphorylation was increased by HP diet only at 30 kg. These data suggested that the HP diet promoted porcine muscle protein accumulation mainly by up-regulating eIF2B1, 4B and 4E rather than 4A1 expression along the growth stages.

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“…In the present study, we constructed a full-length cDNA library from muscle tissue of Wujin pig, which is one of Chinese local breeds in Yunnan, and possesses the traits to accumulate more IMF than commercial pigs. Our group has carried a series of reports including the comparison of meat quality between Wujin and Landrace pigs and the regulation mechanism of nutrition on IMF as well as the impact of H-FABP genotypes and dietary nutritional levels on IMF deposition (Zhao et al 2009;Zhao et al 2010a, b;Wang et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

Zhao

Liu

Pan

et al. 2014

Journal of Integrative Agriculture

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Porcine skeletal muscle genes play a major role in determining muscle growth and meat quality. Construction of a full-length cDNA library is an effective way to understand the expression of functional genes in muscle tissues. In addition, novel genes for further research could be identified in the library. In this study, we constructed a full-length cDNA library from porcine muscle tissue. The estimated average size of the cDNA inserts was 1 076 bp, and the cDNA fullness ratio was 86.2%. A total of 1058 unique sequences with 342 contigs (32.3%) and 716 singleton (67.7%) ESTs were obtained by clustering and assembling. Meanwhile, 826 (78.1%) ESTs were categorized as known genes, and 232 (21.9%) ESTs were categorized as unknown genes. Sixty-five novel porcine genes that exhibit no identity in the TIGR gene index of Sus scrofa and 124 full-length sequences with unknown functions were deposited in the dbEST division of GenBank (accession numbers: EU650784-EU650788; GE843306, GH228978-GH229100). The abundantly expressed genes in porcine muscle tissue were related to muscle fiber development, energy metabolism and protein synthesis. Gene ontology analysis showed that sequences expressed in porcine muscle tissue contained a high percentage of binding activity, catalytic activity, structural molecule activity and motor activity, which involved mainly in metabolic, cellular and developmental process, distributed mainly in intracellular region. The sequence data generated in this study would provide valuable information for identifying porcine genes expressed in muscle tissue and help to advance the study on the structure and function of genes in pigs.

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Low protein diet up-regulate intramuscular lipogenic gene expression and down-regulate lipolytic gene expression in growth–finishing pigs

Cited by 20 publications

References 43 publications

Effects of different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early‐weaned yaks

Effects of different levels of protein supplementary diet on gene expressions related to intramuscular deposition in early‐weaned yaks

The effect of dietary protein levels on the expression of genes coding for four selected protein translation initiation factors in muscle tissue of Wujin pig

Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

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