RETRACTED ARTICLE: The Effect of Zinc Supplementation on Expressed Levels of Peroxisome Proliferator-Activated Receptor Gamma and Glucose Transporter Type 1 Genes in Newborns of Women with Gestational Diabetes Mellitus

Heidarzadeh, Zahra; Samimi, Mansooreh; Seifati, Seyed Morteza; Ashkezari, Mahmood Dehghani; Ahmadi, Shahnaz; Mahmoodi, Samaneh; Aghadavod, Esmat; Jamilian, Mehri; Asemi, Zatollah

doi:10.1007/s12011-016-0788-y

Cited by 9 publications

(4 citation statements)

References 29 publications

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“…We observed altered responses of PPAR-γ expression in cell cultures of offspring from overweight and obese mothers compared to cell cultures of newborns of normal weight mothers. These findings support the notion that an obesogenic intrauterine environment promotes changes in the response to metabolic challenges; miR-155 and miR-221 downregulation is associated with CREB, C/EBP, and PPAR-γ upregulation [20,25]. In accordance to this, PBMCs from offspring of obese mothers cultured without stimuli showed overexpression of PPAR-γ compared to those of offspring from normal weight mothers.…”

Section: Discussionsupporting

confidence: 86%

“…Several metabolic pathways regulated by this nuclear receptor are affected in gestational diabetes, and altered levels of PPAR-γ have been found in fetal and placental tissue in gestational diabetes and other pregnancy disorders [17, 18]. However, information regarding the expression of PPAR-γ, as well as its regulatory mechanisms in neonates and infants, is limited [19, 20]. Because some microRNAs can modulate PPAR-γ expression, they might play a role in shaping of metabolic responses in neonates born to mothers with obesity [10, 21].…”

Section: Introductionmentioning

confidence: 99%

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Changes in PPAR-γ Expression Are Associated with microRNA Profiles during Fetal Programming due to Maternal Overweight and Obesity

Gaytán-Pacheco

Lima‐Rogel

Méndez-Mancilla

et al. 2021

Gynecol Obstet Invest

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Background: There has been a global increase in the prevalence of obesity in pregnant women in recent years. Animal studies have shown that intrauterine environment associated with maternal obesity leads to epigenetic changes. However, the effects of epigenetic changes occurring before birth in response to maternal conditions have not been clearly characterized in humans. Objective: The aim of the study was to analyze peroxisome proliferator-activated receptor (PPAR)-γ expression in cell cultures from newborns from mothers with overweight and obesity, in response to in vitro metabolic challenges and their relationship with microRNA profile and cytokine expression. Methods/Study design: The profile of circulating microRNAs from 72 mother-child pairs (including healthy infants born to normal weight [n = 35], overweight [n = 25], and obese [n = 12] mothers) was determined through real-time PCR, and the PPAR-γ expression in peripheral blood mononuclear cell cultures from offspring was analyzed after in vitro challenges. Results: miR-146a, miR-155, and miR-378a were upregulated in overweight mothers, while miR-378a was upregulated in obese mothers compared to normal weight mothers. In children from overweight mothers, miR-155 and miR-221 were downregulated and miR-146a was upregulated, while offspring of mothers with obesity showed downregulation of miR-155, miR-221, and miR-1301. These microRNAs have direct or indirect relation with PPAR-γ expression. In vitro exposure to high triglyceride and exposure to miR-378a induced a higher expression of PPAR-γ in cells from offspring of mothers with overweight and obesity. In contrast, cells from offspring of mothers with obesity cultured with high glucose concentrations showed PPAR-γ downregulation. IL-1ß, IL-6, and TNF-α expression in cells of offspring of overweight and obese mothers differed from that of offspring of normal weight mothers. Limitation of our study is the small sample size. Conclusion: The blood microRNA profile, and in vitro PPAR-γ and inflammatory cytokine expression in cells of newborn infants are associated with maternal obesity indicating that epigenetic marks may be established during intrauterine development. Key Message: Neonatal microRNA profile is associated with maternal weight. Neonatal microRNA profile is independent of maternal microRNA profile. PPAR-γ expression in newborn cell cultures is affected by maternal weight

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Changes in PPAR-γ Expression Are Associated with microRNA Profiles during Fetal Programming due to Maternal Overweight and Obesity

Gaytán-Pacheco

Lima‐Rogel

Méndez-Mancilla

et al. 2021

Gynecol Obstet Invest

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“…PPARs are ligand-activated transcription factors, and PPARγ regulates fatty acid, glucose, and energy homeostasis ( 38 ). Studies have shown that Zn supplementation could increase PPARγ expression ( 39 ), since the PPAR-DNA binding seems to occur through ZnDs ( 16 ).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the obesity-related biomarker, peroxisome proliferator-activated receptor-gamma (PPARγ), is a nuclear receptor protein that regulates fatty acid and glucose homeostasis. Zn supplementation may increase PPARγ expression, resulting from the two ZnDs that can bind to DNA ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

Novel Zinc-Related Differentially Methylated Regions in Leukocytes of Women With and Without Obesity

et al. 2022

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IntroductionNutriepigenetic markers are predictive responses associated with changes in “surrounding” environmental conditions of humans, which may influence metabolic diseases. Although rich in calories, Western diets could be linked with the deficiency of micronutrients, resulting in the downstream of epigenetic and metabolic effects and consequently in obesity. Zinc (Zn) is an essential nutrient associated with distinct biological roles in human health. Despite the importance of Zn in metabolic processes, little is known about the relationship between Zn and epigenetic. Thus, the present study aimed to identify the epigenetic variables associated with Zn daily ingestion (ZnDI) and serum Zinc (ZnS) levels in women with and without obesity.Materials and MethodsThis is a case-control, non-randomized, single-center study conducted with 21 women allocated into two groups: control group (CG), composed of 11 women without obesity, and study group (SG), composed of 10 women with obesity. Anthropometric measurements, ZnDI, and ZnS levels were evaluated. Also, leukocyte DNA was extracted for DNA methylation analysis using 450 k Illumina BeadChips. The epigenetic clock was calculated by Horvath method. The chip analysis methylation pipeline (ChAMP) package selected the differentially methylated regions (DMRs).ResultsThe SG had lower ZnS levels than the CG. Moreover, in SG, the ZnS levels were negatively associated with the epigenetic age acceleration. The DMR analysis revealed 37 DMRs associated with ZnDI and ZnS levels. The DMR of PM20D1 gene was commonly associated with ZnDI and ZnS levels and was hypomethylated in the SG.ConclusionOur findings provide new information on Zn's modulation of DNA methylation patterns and bring new perspectives for understanding the nutriepigenetic mechanisms in obesity.

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Zinc supplementation for improving pregnancy and infant outcome

Carducci

Keats

Bhutta

2021

Cochrane Database of Systematic Reviews

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RETRACTED ARTICLE: The Effect of Zinc Supplementation on Expressed Levels of Peroxisome Proliferator-Activated Receptor Gamma and Glucose Transporter Type 1 Genes in Newborns of Women with Gestational Diabetes Mellitus

Cited by 9 publications

References 29 publications

Changes in PPAR-γ Expression Are Associated with microRNA Profiles during Fetal Programming due to Maternal Overweight and Obesity

Changes in PPAR-γ Expression Are Associated with microRNA Profiles during Fetal Programming due to Maternal Overweight and Obesity

Novel Zinc-Related Differentially Methylated Regions in Leukocytes of Women With and Without Obesity

Zinc supplementation for improving pregnancy and infant outcome

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