Rapid cell sheet detachment using spin-coated pNIPAAm films retained on surfaces by an aminopropyltriethoxysilane network

Patel, Nikul G.; Cavicchia, John P.; Zhang, Ge; Newby, Bi‐min Zhang

doi:10.1016/j.actbio.2012.03.031

Cited by 52 publications

(50 citation statements)

References 38 publications

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“…Human mesenchymal stem cells (hMSCs) were cultured on a thermoresponsive surface fabricated using our newly developed spin coating technique. 17 The detachment of hMSC sheets was achieved by replacing cell culture medium with fresh cold medium (4 °C). No residual cells were detected after cell sheet detachment.…”

Section: Resultsmentioning

confidence: 99%

Stacked stem cell sheets enhance cell-matrix interactions

Patel

Zhang

2014

Organogenesis

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Section: Resultsmentioning

confidence: 99%

Stacked stem cell sheets enhance cell-matrix interactions

Patel

Zhang

2014

Organogenesis

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“…Examples include, but are not limited to, MSCs, bovine endothelial cells, Madin-Darby canine kidney (MDCK) cells, and NIH3T3 fibroblasts. 98,[101][102][103] Graft copolymers comprised of PNIPAAm and PEG have also been employed to alter cell behavior dynamically. Fibroblasts adhered, spread, and proliferated on PNIPAM-PEG copolymers at 37°C, but became completely detached and were recovered as single cell sheets upon temperature reduction to 25°C.…”

Section: Temperaturementioning

confidence: 99%

“…Consequently, cell culture substrates coated with thermoresponsive polymers can be used to harvest cell sheets with the undamaged ECM, simply by altering substrate temperatures around the LCSTs. [97][98][99] Cell sheets formed with the aid of thermoresponsive polymers, in contrast to the use of enzymes, minimize the damage to cells and their ECM, thus preserving biological functions.…”

Section: Temperaturementioning

confidence: 99%

Dynamic Manipulation of Hydrogels to Control Cell Behavior: A Review

Vats

Benoit²

2013

Tissue Engineering Part B: Reviews

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For many tissue engineering applications and studies to understand how materials fundamentally affect cellular functions, it is important to have the ability to synthesize biomaterials that can mimic elements of native cellextracellular matrix interactions. Hydrogels possess many properties that are desirable for studying cell behavior. For example, hydrogels are biocompatible and can be biochemically and mechanically altered by exploiting the presentation of cell adhesive epitopes or by changing hydrogel crosslinking density. To establish physical and biochemical tunability, hydrogels can be engineered to alter their properties upon interaction with external driving forces such as pH, temperature, electric current, as well as exposure to cytocompatible irradiation. Additionally, hydrogels can be engineered to respond to enzymes secreted by cells, such as matrix metalloproteinases and hyaluronidases. This review details different strategies and mechanisms by which biomaterials, specifically hydrogels, can be manipulated dynamically to affect cell behavior. By employing the appropriate combination of stimuli and hydrogel composition and architecture, cell behavior such as adhesion, migration, proliferation, and differentiation can be controlled in real time. This three-dimensional control in cell behavior can help create programmable cell niches that can be useful for fundamental cell studies and in a variety of tissue engineering applications.

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“…The network can be tightened when the APTES molecules cross-link with each other under thermal annealing to firmly lock the diffused polymer chains in the network. [6][7][8] In addition, to further enhance the coupling, hydrolyzed APTES, with its amino and hydroxyl groups, might allow acid-base (AB) (e.g., H-bonding) interactions with the amide group in pNIPAAm (Ref. 21) as well as vander Waals interactions.…”

Section: Introductionmentioning

confidence: 99%

“…Polymer retention is often achieved by using different grafting methods [1][2][3][4][5] or by utilizing coupling agents/adhesion promoters. [6][7][8][9] When silanes are used as the adhesion promoters, they are normally deposited on the inorganic/metal surface and cured to form a network, which is expected to interdiffuse with the polymer once the polymer is placed on it. [10][11][12] Thus, an effective adhesion promoter should consist of appropriate groups that would react/interact with the polymer as well as exhibit a suitable solubility with the polymer to form an interdiffused interphase.…”

Section: Introductionmentioning

confidence: 99%

Retention of poly(N-isopropylacrylamide) on 3-aminopropyltriethoxysilane

et al. 2017

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Silane coupling agents are commonly employed to link an organic polymer to an inorganic substrate. One of the widely utilized coupling agents is 3-aminopropyltriethoxy silane (APTES). In this study, the authors investigated the ability of APTES to retain thermo-responsive poly(N-isopropylacrylamide) (pNIPAAm) on hydroxylated surfaces such as glass. For comparison purposes, the authors also evaluated the retention behaviors of (1) polystyrene, which likely has weaker van der Waals interactions and acid-base interactions (contributed by hydrogen-bonding) with APTES, on APTES as well as (2) pNIPAAm on two other silane coupling agents, which have similar structures to APTES, but exhibit less interaction with pNIPAAm. Under our processing conditions, the stronger interactions, particularly hydrogen bonding, between pNIPAAm and APTES were found to contribute substantially to the retention of pNIPAAm on the APTES modified surface, especially on the cured APTES layer when the interpenetration was minimal or nonexistent. On the noncured APTES layer, the formation of an APTES-pNIPAAm interpenetrating network resulted in the retention of thicker pNIPAAm films. As demonstrated by water contact angles [i.e., 7 -15 higher at 40 C, the temperature above the lower critical solution temperature (LCST) of 32 C for pNIPAAm, as compared to those at 25 C] and cell attachment and detachment behaviors (i.e., attached/spread at 37 C, above LCST; detached at 20 C, below LCST), the retained pNIPAAm layer (6-15 nm), on both noncured and cured APTES, exhibited thermo-responsive behavior. The results in this study illustrate the simplicity of using the coupling/adhesion promoting ability of APTES to retain pNIPAAm films on hydroxylated substrates, which exhibit faster cell sheet detachment ( 30 min) as compared to pNIPAAm brushes (in hours) prepared using tedious and costly grafting approaches. The use of adhesion promoters to retain pNIPAAm provides an affordable alternative to current thermo-responsive supports for cell sheet engineering and stem cell therapy applications.

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Rapid cell sheet detachment using spin-coated pNIPAAm films retained on surfaces by an aminopropyltriethoxysilane network

Cited by 52 publications

References 38 publications

Stacked stem cell sheets enhance cell-matrix interactions

Stacked stem cell sheets enhance cell-matrix interactions

Dynamic Manipulation of Hydrogels to Control Cell Behavior: A Review

Retention of poly(N-isopropylacrylamide) on 3-aminopropyltriethoxysilane

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