Rapid Bioassay for Clostridium Botulinum Type-E Toxins by Intravenous Injection Into Mice

Abstract

A bioassay method for Clostridium botulinum type-E toxins has

Responses of Clostridium Botulinum Type B and E Progenitor Toxins to Some Clostridial Sulfhydryl-Dependent Proteases

“…The reaction mixture was incubated at 35 C for 30 min. The toxicities before and after enzyme treatment in terms of ip LD50/ml were estimated by the time-to-death method (Boroff and Fleck, 1966) from the same standard curve prepared with each trypsinactivated progenitor toxin, although the toxicity of unactivated type E progenitor toxin would be overestimated by this method (Sakaguchi, Sakaguchi and Kondo, 1968). The percentage of the toxicity resulting from treatment with a given clostridial protease to that resulting from trypsinization was used to express the activation potency.…”

Cross Reaction in Reversed Passive Hemagglutination Between Clostridium Botulinum Type a and B Toxins and Its Avoidance by the Use of Antitoxic Component Immunoglobulin Isolated by Affinity Chromatography

“…Toxin potencies: The time-to-death method (Boroff and Fleck, 1966;Sakaguchi, Sakaguchi and Kondo, 1968) was used.…”

Detection And Identification of Clostridium botulinum Neurotoxins

“…This problem has been alleviated to some extent by using intravenous injection (tail vein) of a single dilution of a preparation into several mice and determining the time to death (3,61). The LDsolml of the sample is calculated from the linear relationship between LDso and time to death determined for a standard toxin preparation.…”