Cross Reaction in Reversed Passive Hemagglutination Between Clostridium Botulinum Type a and B Toxins and Its Avoidance by the Use of Antitoxic Component Immunoglobulin Isolated by Affinity Chromatography

Sakaguchi, Genji; Sakaguchi, Sumiko; Kozaki, Shunji; Sugii, Shunji; Oishi, Isao

doi:10.7883/yoken1952.27.161

Cited by 44 publications

(28 citation statements)

References 6 publications

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“…A week after the final injection, the mice were bled and cell hybridization was performed. The sera from the immunized mice reached titers of 25,600 in the passive hemagglutination test (9,22). Production of hybridoma and preparation of MAb (4).…”

Section: Methodsmentioning

confidence: 99%

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Production and characterization of monoclonal antibodies to Clostridium perfringens enterotoxin

Horiguchi¹,

Uemura²,

Kamata³

et al. 1986

Infect Immun

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Four hybridoma cell lines producing monoclonal antibodies to Clostridium perfringens enterotoxin were established by fusion of mouse myeloma and spleen cells obtained from mice immunized with the enterotoxin and its toxoid. An enzyme-linked immunosorbent assay indicated that the two antibodies, 2-B-4 and 3-G-10, bound to those regions that were located close each other; the others, 3-B-2 and 2-H-2, bound to other independent regions on the enterotoxin. Release of 51Cr from Vero cells with the enterotoxin was inhibited by either 2-B-4 or 3-G-10, both of which inhibited the binding of 125I-labeled enterotoxin to the cells. Neither binding nor cytotoxicity of the enterotoxin was affected by 2-H-2; 3-B-2 only barely inhibited the binding but neutralized the enterotoxin shown by 51Cr release. It seems justified to conclude that 3-B-2 blocks the toxic action after the enterotoxin has bound to Vero cells.

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Section: Methodsmentioning

confidence: 99%

“…MAbs were purified by DEAE Affi-Gel blue (Bio-Rad Laboratories, Richmond, Calif.) chromatography (1). Polyclonal antibodies were purified by affinity chromatography on cyanogen bromide-Sepharose 4B (Pharmacia Fine Chemicals, Uppsala, Sweden) coupled with the enterotoxin (22).…”

Section: Methodsmentioning

confidence: 99%

Production and characterization of monoclonal antibodies to Clostridium perfringens enterotoxin

Horiguchi¹,

Uemura²,

Kamata³

et al. 1986

Infect Immun

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“…Generally, ELISA systems based on pAb show a higher tendency for crossreactivity compared to ELISAs using mAb. Especially, if the pAb has been generated against BoNT complexes or bacterial supernatants rather than the pure holotoxins, high titers against accessory proteins or unrelated proteins have been observed which can result in substantial cross-reactivity (Sakaguchi et al 1974;Sugiyama et al 1974;Betley and Sugiyama 1979;Notermans et al 1982b;Sakaguchi 1982;Dezfulian et al 1984;Kumar et al 1994;Ferreira 2001). It has also been noticed that culture supernatants of C. botulinum shared antigens found in supernatants of other clostridia (Poxton 1984;Poxton and Byrne 1984) and gave rise to cross-reactivity with nontoxogenic clostridia (Lewis et al 1981;Thomas 1991).…”

Section: Selected Examples Of Different Elisa Formatsmentioning

confidence: 99%

Complexity of Botulinum Neurotoxins: Challenges for Detection Technology

Dorner

Schulz

Kull

et al. 2012

Current Topics in Microbiology and Immunology

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The detection of botulinum neurotoxins (BoNT) is extremely challenging due to their high toxicity and the multiple BoNT variants. To date, seven serotypes with more than 30 subtypes have been described, and even more subtypes are expected to be discovered. The fact that the BoNT molecules are released as large complexes of different size and composition adds further complexity to the issue. Currently, in the diagnostics of botulism, the mouse bioassay (MBA) is still considered as gold standard for the detection of BoNT in complex sample materials. Over the years, different functional, immunological, and spectrometric assays or combinations thereof have been developed, supplemented by DNA-based assays for the detection of the organism. In this review, advantages and limitations of the current technologies will be discussed, highlighting some of the intricacies of real sample analysis.

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“…Several immunological procedures (i.e. hemagglutination, radioimmunoassay, immunodiffusion) have been developed (Boroff and Shu-Chen, 1973;Johnson et al, 1966;Sakaguchi et al, 1974;Vemilyea, Walker and Aynes, 1968). A recent technique for detection of specific proteins is the enzyme-linked immunosorbent assay (ELISA).…”

Section: Introductionmentioning

confidence: 99%

Enzyme Linked Immunosorbent Assay (Elisa) for Detection of Clostridium Botulinum Type B Toxin

Kozaki

Dufrenne

Hagenaars

et al. 1979

JJMSB

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Cross Reaction in Reversed Passive Hemagglutination Between Clostridium Botulinum Type a and B Toxins and Its Avoidance by the Use of Antitoxic Component Immunoglobulin Isolated by Affinity Chromatography

Cited by 44 publications

References 6 publications

Production and characterization of monoclonal antibodies to Clostridium perfringens enterotoxin

Production and characterization of monoclonal antibodies to Clostridium perfringens enterotoxin

Complexity of Botulinum Neurotoxins: Challenges for Detection Technology

Enzyme Linked Immunosorbent Assay (Elisa) for Detection of Clostridium Botulinum Type B Toxin

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