Rapid attainment of a doubled haploid line from transgenic maize (Zea mays L.) plants by means of anther culture

Aulinger, I. E.; Peter, Stefan; Schmid, Jürg; Stamp, P.

doi:10.1079/ivp2002386

Cited by 19 publications

(13 citation statements)

References 34 publications

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“…This somaclonal variation has been detected with RAPDs (Raimondi et al 2001), producing a changes similar to the obtained by Eimert et al (2003). The EST-SSRs have been used to determine the origin of the regenerated plantlets from anther culture in different species such as rice (Lapitan et al 2009), apple (Höfer et al 2002;Vanwynsberghe et al 2005;Okada et al 2009), corn (Aulinger et al 2003), soy (Rodrigues et al 2004), potato (Veilleux et al 1995;Chani et al 2000), coconut (Perera et al 2008), orange (Cao et al 2011) or eggplant (Salas et al 2011).…”

Section: Discussionmentioning

confidence: 89%

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Regalado

Martín

Madrid

et al. 2015

Plant Cell Tiss Organ Cult

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Anther culture is used to develop asparagus ''super-male'' (di-haploids) in asparagus, which can be used to develop ''all-male'' varieties, by crossing them with suitable females; their progenies will be formed only by males which is advantageous for producers. This report describe a new anther culture protocol adapted to ''Morado de Huétor'', a Spanish tetraploid landrace, and studied the different factors involved in callus proliferation success from anther explants such as the microspore development stage, or the type of stress used to induce the symmetric division of the microspores, to obtain a high success rate (90 %). For plantlets regenerates from anther culture (PRACs) regeneration we develop a proliferation media supplemented with a combination of pCPA and BA able to induce callus proliferation and plantlet regeneration in the same step in a 50 % of calli, simplifying the procedure. The high percentage of heterozygous male recovery, originated from somatic cells, is an important problem in the anther culture, and to elucidate the origin of PRACs we have combined different tools: ploidy analysis, characterization with the linked sex-marker Asp1-T7 and with ESTSRRs. We can establish that 50 % of PRACs obtained in this work were regenerated from diploid microspores of ''Morado de Huétor'', regenerating diploid, di-diploid and tetra-diploid plantlets. The di-diploids males (MMmm) would generate a ratio male:female of 5:1 (83.3 %) and the tetra-diploid males (MMMMmmmm) a ratio male:female of 69:1 (98.6 %), so the tetra-diploid males could be considered ''super-males'' and be used to develop ''all-male'' varieties of ''Morado de Huétor''.

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Section: Discussionmentioning

confidence: 89%

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Regalado

Martín

Madrid

et al. 2015

Plant Cell Tiss Organ Cult

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“…Callus is a heterogeneous mass of cells having different morphogenetic potentials. In anther culture in vitro of cereals two types of calli are present -morphogenic and non-morphogenic -able and enable to form androgenic plants, respectively [2,8,14,16,25]. Morphogenesis in the anthers of each of studied wheat cultivars was characterized by its own peculiarities ( fig.).…”

Section: Resultsmentioning

confidence: 99%

Phytohormonal Regulation of in Vitro Formation of Wheat Androgenic Structures

Seldimirova¹,

D.Yu.²,

Galin

et al. 2016

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Seldimirova O.A., Zaytsev D.Yu., Galin I.R., Kruglova N.N. AbstractThe phenomenon of androgenesis in vitro is widely used in biotechnological investigations of spring wheat. When solving the specific biotechnological problems it is important to know what Seldimirova O.A., Zaytsev D.Yu., Galin I.R., Kruglova N.N. Phytohormonal regulation of in vitro formation of wheat androgenic structures // Сетевой журнал «Научный результат». Серия «Физиология». -Т.2, №1(7), 2016. 4 Серия ФИЗИОЛОГИЯ PHISIOLOGY Series kind of morphogenesis pathway in vitro will result in androgenic plants. This research is devoted to developing a method of phytohormonal regulation of in vitro formation of a certain type of wheat androgenic structures. Using the method of ELISA it was shown that the induction of certain sporophytic morphogenesis pathway in vitro of anther haploid cells -microspores depends on both the content of endogenous auxin IAA in anthers before inoculating them onto induction medium, and the concentration of exogenous auxin 2,4-D in this medium. The obtained data confirms the principle possibility of regulation of ways of getting androgenic regenerants in vitro by selecting the optimal balance of endogenous and exogenous auxins.

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“…In caraway, it appears that there is an indirect regeneration via a callus stage. A prolonged callus phase and the additional in vitro culture period may lead to the accumulation of mutations and to additional genetic variation among DH plants (Aulinger et al 2003). Bartošová et al (2005) described possible diploidization after induction of microspores.…”

Section: Discussionmentioning

confidence: 99%

Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi L.)

Smýkalová

Horáček

Kubošiová

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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Plant regeneration was obtained from cultured anthers and hypocotyl segments of caraway (Carum carvi L.). Microspore-and somatic tissue-derived embryos were compared by observation of the regeneration process under identical induction conditions. Fluorescent microscopy with DAPI staining showed initiation of cell divisions and formation of embryogenic callus and somatic embryos from anther sacs, with production of embryos of both microspore and somatic origin. Induction of somatic embryos from hypocotyl-derived callus was also demonstrated. Isozyme native polyacrylamide gel electrophoresis was used to identify haploids and doubled haploids, and to determine the frequency of spontaneous diploidization of regenerated plants of microspore origin. Donor plants (2n= 20) and their anther-derived derivative plants (n=10, 2n= 20, 4n=40) in callus stage or leafy rosette stage were compared. The esterase (EST) band patterns of regenerated plants differed from the heterozygous parental material, suggesting that the regenerated plants were microsporederived haploid/doubled haploid plants. The similar profile of EST bands between the diploid anther-derived plants and a sample of the donor plants corresponded to a somatic regeneration pathway. Although the selected induction conditions revealed no preference for induction of microspore embryogenesis, the anther culture protocol established for caraway utilizing isozyme segregating EST loci markers is suitable for DH production.

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Rapid attainment of a doubled haploid line from transgenic maize (Zea mays L.) plants by means of anther culture

Cited by 19 publications

References 34 publications

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Phytohormonal Regulation of in Vitro Formation of Wheat Androgenic Structures

Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi L.)

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