Rapid and Specific Polymerase Chain Reaction-Enzyme Linked Immunosorbent Assay for Detection of Escherichia coli LT Toxin From Clinical Isolates

Esfandiari, Pantea; Amani, Jafar; Fouladi, A A Imani; Nazarian, Javad; Mirhosseini, Seyed Ali; Moghimi, Ebrahim

doi:10.5812/archcid.36261

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…As a pre-enrichment medium, and incubated at 37°C for 16 hours. About 0.1 ml of the pre-enriched sample was transferred using a pipette into a tube containing 10 ml of tetrathionate broth (Himedia, India)(1:9) used for selective enrichment of samples, The samples were mixed well by shaking and incubated at 37°C for 18 hours [11].…”

Section: Sample Collectionmentioning

confidence: 99%

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Detection of gene stn in some non-typhoidal Salmonella spp. which isolated from patients with diarrhea

Tawfiq¹

2019

Tikrit J. Pure Sci.

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The present study was conducted to estimate the prevalence of Non-typhoidal Salmonella spp. From April 2017 to September 2017 approximately 222 samples from diarrhoeic stool of human ages between one month till above sixty years were collected in Rizkary , children's and Kirkuk hospitals to determine the prevalence stn gene among Non-typhoidal Salmonella spp(S. enteritidis). A total of 220 samples were collected, The colonial morphology, staining, microscopical examination, cultural media as well as biochemical characteristics of the isolates found out the presence (72) 32.7% out of 220 salmonella species isolates, (41) 57% were belong to NTS from 72 isolates while S. enteritidis were 28(68.2%) from 41 isolates of NTS. PCR assay was carried out to detect the presence of stn gene, (24) 85.7% isolates of S. enteritidis contained the gene among 41 isolates of S. enteritidis. 100% of isolates were susceptible to Norfloxacin, while 16.7% were sensitive to Cefotaxime, 70% of isolates appeared multidrug resistance. 32.1% infections recorded in ages above sixty,25% age less than one year.

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Section: Sample Collectionmentioning

confidence: 99%

“…Salmonella enterotoxin alters vascular permeability in the skin, increases cyclic AMP levels and exert substantial effects on water and electrolyte during intestinal infection. [11].…”

Section: Introductionmentioning

confidence: 99%

Detection of gene stn in some non-typhoidal Salmonella spp. which isolated from patients with diarrhea

Tawfiq¹

2019

Tikrit J. Pure Sci.

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“…Enterotoxigenic Escherichia coli (ETEC) is a significant cause of diarrhea in both humans and animals ( Francis, 2002 ; Esfandiari et al, 2017 ). Post-weaning diarrhea (PWD) typically manifests within 2 weeks following weaning, and is characterized by clinical signs including diarrhea, dehydration, and mortality.…”

Section: Introductionmentioning

confidence: 99%

Genomic traits of multidrug resistant enterotoxigenic Escherichia coli isolates from diarrheic pigs

Jiang-hong

Huang

et al. 2023

Front. Microbiol.

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Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) infections poses a significant challenge in global pig farming. To address this issue, the study was conducted to identify and characterize 19 ETEC isolates from fecal samples of diarrheic pigs sourced from large-scale farms in Sichuan Province, China. Whole-genome sequencing and bioinformatic analysis were utilized for identification and characterization. The isolates exhibited substantial resistance to cefotaxime, ceftriaxone, chloramphenicol, ciprofloxacin, gentamicin, ampicillin, tetracycline, florfenicol, and sulfadiazine, but were highly susceptible to amikacin, imipenem, and cefoxitin. Genetic diversity among the isolates was observed, with serotypes O22:H10, O163orOX21:H4, and O105:H8 being dominant. Further analysis revealed 53 resistance genes and 13 categories of 195 virulence factors. Of concern was the presence of tet(X4) in some isolates, indicating potential public health risks. The ETEC isolates demonstrated the ability to produce either heat-stable enterotoxin (ST) alone or both heat-labile enterotoxin (LT) and ST simultaneously, involving various virulence genes. Notably, STa were linked to human disease. Additionally, the presence of 4 hybrid ETEC/STEC isolates harboring Shiga-like toxin-related virulence factors, namely stx2a, stx2b, and stx2e-ONT-2771, was identified. IncF plasmids carrying multiple antimicrobial resistance genes were prevalent, and a hybrid ETEC/STEC plasmid was detected, highlighting the role of plasmids in hybrid pathotype emergence. These findings emphasized the multidrug resistance and pathogenicity of porcine-origin ETEC strains and the potential risk of epidemics through horizontal transmission of drug resistance, which is crucial for effective control strategies and interventions to mitigate the impact on animal and human health.

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Review on pathogenicity mechanism of enterotoxigenic Escherichia coli and vaccines against it

Mirhoseini

Amani

Nazarian

2018

Microbial Pathogenesis

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Rapid and Specific Polymerase Chain Reaction-Enzyme Linked Immunosorbent Assay for Detection of Escherichia coli LT Toxin From Clinical Isolates

Cited by 4 publications

References 31 publications

Detection of gene stn in some non-typhoidal Salmonella spp. which isolated from patients with diarrhea

Detection of gene stn in some non-typhoidal Salmonella spp. which isolated from patients with diarrhea

Genomic traits of multidrug resistant enterotoxigenic Escherichia coli isolates from diarrheic pigs

Review on pathogenicity mechanism of enterotoxigenic Escherichia coli and vaccines against it

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