Haemophilus parasuis, the causative agent of Glässer's disease, has been reported widely, but seldom is known about its epidemiology in Sichuan province, China. The objective was to reveal the prevalence and distribution of H.parasuis in the area. Widely sampling and isolation was performed initially and following serotyping multiplex PCR (serotyping-mPCR) combined with agar gel diffusion (GD) was subjected to these strains. From January 2014 to May 2016, 254 H.parasuis field strains were isolated from 576 pigs with clinical symptoms, for the frequence of 44.10%. Statistically significant differences of infection incidence were found in three age groups and seasons. Serovars 5(25.98%) and 4(23.62%) were the most prevalent and non-typeable isolates accounted for 7.87%. In geographical distribution, serovars 5 and 4 were prepotent in both major two parts of Sichuan province.The results confirmed the compound approach was dependable and revealed the diversity and distribution of serovars in Sichuan province, which was promising to know relevant vaccinal candidates and further prevention.PeerJ Preprints | https://doi.org/10.7287/peerj.preprints.2805v1 | CC BY 4.0 Open Access | rec: 15
Dental caries severely hinders efficient access to adequate energy in wildlife. Different food supplies will develop characteristic plaque, and the microorganisms of these plaque are closely related to dental health. Here, plaque samples from panda cubs with caries and caries-free were collected for 16S rRNA high-throughput sequencing. All sequences clustered into 337 operational taxonomic units (OTUs; 97% identity), representing 268 independent species belonging to 189 genera, 98 families, 51 orders, 24 classes, and 13 phyla. Two groups shared 218 OTUs, indicating the presence of a core plaque microbiome. α diversity analysis showed that the microbial diversity in plaques with caries exceeded that of caries-free. The dominant phyla of plaque microbiota included Proteobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Actinobacteria. The dominant genera included unclassified Neisseriaceae, Actinobacillus, Lautropia, Neisseria, Porhyromonas, unclassified Pasteurellaceae, Moraxella, Streptococcus, Bergeywlla and Capnocytophaga. β diversity analysis showed that the plaque microbial community structure was different between two groups. Using LEfSe analysis, 19 differentially abundant taxa were identified as potential biomarkers. Finally, function predictions analysis showed All the energy related metabolic pathways on KEGG level 2 were enriched in caries-active group. Consistent with the mainstream caries-causing narrative, our results illuminate the lack of information regarding the oral microflora composition and function within giant panda cubs.
Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) infections poses a significant challenge in global pig farming. To address this issue, the study was conducted to identify and characterize 19 ETEC isolates from fecal samples of diarrheic pigs sourced from large-scale farms in Sichuan Province, China. Whole-genome sequencing and bioinformatic analysis were utilized for identification and characterization. The isolates exhibited substantial resistance to cefotaxime, ceftriaxone, chloramphenicol, ciprofloxacin, gentamicin, ampicillin, tetracycline, florfenicol, and sulfadiazine, but were highly susceptible to amikacin, imipenem, and cefoxitin. Genetic diversity among the isolates was observed, with serotypes O22:H10, O163orOX21:H4, and O105:H8 being dominant. Further analysis revealed 53 resistance genes and 13 categories of 195 virulence factors. Of concern was the presence of tet(X4) in some isolates, indicating potential public health risks. The ETEC isolates demonstrated the ability to produce either heat-stable enterotoxin (ST) alone or both heat-labile enterotoxin (LT) and ST simultaneously, involving various virulence genes. Notably, STa were linked to human disease. Additionally, the presence of 4 hybrid ETEC/STEC isolates harboring Shiga-like toxin-related virulence factors, namely stx2a, stx2b, and stx2e-ONT-2771, was identified. IncF plasmids carrying multiple antimicrobial resistance genes were prevalent, and a hybrid ETEC/STEC plasmid was detected, highlighting the role of plasmids in hybrid pathotype emergence. These findings emphasized the multidrug resistance and pathogenicity of porcine-origin ETEC strains and the potential risk of epidemics through horizontal transmission of drug resistance, which is crucial for effective control strategies and interventions to mitigate the impact on animal and human health.
Haemophilus parasuis, the causative agent of Glässer's disease, has been reported widely, but seldom is known about its epidemiology in Sichuan province, China. The objective was to reveal the prevalence and distribution of H.parasuis in the area. Widely sampling and isolation was performed initially and following serotyping multiplex PCR (serotyping-mPCR) combined with agar gel diffusion (GD) was subjected to these strains. From January 2014 to May 2016, 254 H.parasuis field strains were isolated from 576 pigs with clinical symptoms, for the frequence of 44.10%. Statistically significant differences of infection incidence were found in three age groups and seasons. Serovars 5(25.98%) and 4(23.62%) were the most prevalent and non-typeable isolates accounted for 7.87%. In geographical distribution, serovars 5 and 4 were prepotent in both major two parts of Sichuan province.The results confirmed the compound approach was dependable and revealed the diversity and distribution of serovars in Sichuan province, which was promising to know relevant vaccinal candidates and further prevention.PeerJ Preprints | https://doi.org/10.7287/peerj.preprints.2805v1 | CC BY 4.0 Open Access | rec: 15
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