Rapid and simple determination of adenine and guanine in DNA extract by micellar electrokinetic chromatography with indirect laser-induced fluorescence detection

Wang, Weiping; Zhou, Lei; Wang, Shumin; Luo, Zhihao; Hu, Zhide

doi:10.1016/j.talanta.2007.06.047

Cited by 53 publications

(40 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the detection of DNA bases is of great significance for clinical diagnosis as well as insight into fundamental mechanisms of genetic information (Jelen et al, 2009;Abbaspour and Ghaffarinejad, 2010). In recent years, several analytical methods have been developed to detect DNA bases, such as microchip capillary electrophoresis (Wang et al, 2004), flow injection chemiluminescence (Liu and Xue, 2006), ionpairing liquid chromatography (Ganzera et al, 2006), laser-induced fluorescence detection (Wang et al, 2008), and micellar electrokinetic chromatography . Although these methods exhibit some merits and advantages, expensive instruments, complicated operations, or time-consuming sample pretreatments are usually involved (Sun et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of guanine, adenine, thymine and cytosine at choline monolayer supported multiwalled carbon nanotubes film

Wang

Dai

et al. 2011

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of guanine, adenine, thymine and cytosine at choline monolayer supported multiwalled carbon nanotubes film

Wang

Dai

et al. 2011

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…It can influence not only the fluorescence intensity of fluorescein, but also the electroosmotic flow (EOF) mobility and the electrophoretic mobilities of solutes. 6,22 The effect of the running buffer pH on separating the types of nitrophenols is displayed in Fig. 2(A).…”

Section: Effect Of Ph Injection Time Borate Concentration and Applimentioning

confidence: 99%

“…(1) and S/N = 3. 6,7 As such, the determination of LOD was simply based on S/N = 3 in this work, as displayed in Table 1.…”

Section: Theory Of Indirect Fluorescence Detectionmentioning

confidence: 99%

“…3,4 The detection mechanism can be explained by the displacement of a visualizing reagent from the sample zone to maintain local charge neutrality. 1,[5][6][7] Currently, one of the most sensitive detection techniques in CE is laser-induced fluorescence (LIF) detection, 8 which was first introduced into CE by Zare et al 9 In order to detect compounds without native fluorescence, indirect LIF [10][11][12][13][14] can be applied to determine these compounds. However, most indirect LIF detections possess the disadvantages of high background noise, using single-wavelength detection and requiring relatively expensive laser equipment.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Indirect Fluorescence Detection of p-Nitrophenol, 2,4-Dinitrophenol and Trinitrophenol by Micellar Electrokinetic Capillary Chromatography with In-column Optical-fiber LED-induced Fluorescence Detection

et al. 2011

View full text Add to dashboard Cite

Enhanced indirect fluorescence detection with in-column optical-fiber LED-induced fluorescence (LED-IF) detection has been developed for the simultaneous separation and determination of p-nitrophenol, 2,4-dinitrophenol and trinitrophenol. The instrumental set-up is simple, cost-effective and the detection method is stable. Different experimental parameters have been studied. The optimum conditions were determined as: running buffer (pH 9.1) composed of 10 mM borate, 80 mM sodium dodecylsulfate and 0.18 μM fluorescein (as a background reagent); applied voltage of 15 kV; working temperature of 25.0 C. The limits of detection (S/N = 3) were 11, 18 and 16 μM for p-nitrophenol, 2,4-dinitrophenol and trinitrophenol, respectively. The nitrophenols were completely separated in 13 min and the number of theoretical plates for p-nitrophenol, 2,4-dinitrophenol and trinitrophenol were 8.1 × 10 6 , 6.5 × 10 6 and 8.4 × 10 6 , respectively. The RSDs (n = 6) for the migration time and the peak area were better than 0.85 and 0.91%, respectively. Our proposed method possesses the advantages of rapidness and good separation efficiency.

show abstract

“…In recent years, a series of methods, such as micellar electrokinetic chromatography (MEKC), followed by indirect laser-induced fluorescence detection (ILIFD) [9]; HPLC and capillary electrophoresis (CE), followed by UV [10][11][12][13][14]; ion-pairing liquid chromatography (IPLC) [15]; flow injection-chemiluminescence (CL) [16]; and spectroscopic methods, were developed for the detection of guanine and adenine [17,18]. These methods have gained interest from the researchers in life sciences due to their high efficiency and small sample size requirement, but they need complicated instruments and time-consuming sample pretreatment.…”

Section: Introductionmentioning

confidence: 99%

Electrodeposit nano-copper oxide on glassy carbon electrode for simultaneous detection of guanine and adenine

Zhang

Liang

et al. 2012

J Appl Electrochem

View full text Add to dashboard Cite

An electrochemical sensor was fabricated and used to simultaneously detect guanine and adenine. In this study, nano-copper oxide-modified glassy carbon electrode (nano-copper oxide/GCE) was prepared by electrodeposition. The nano-copper oxide/GCE was characterized by electrochemical impedance spectroscopy and scanning electron microscopy. The fabricated nano-copper oxide/ GCE sensor exhibited sensitive response to guanine and adenine in 0.1 M PBS (pH 7.0). The anodic peak currents were linear with the guanine and adenine concentrations over the range of 0.05-1.2 lM with the correlation coefficients of 0.9997 and 0.9993, respectively, and the corresponding detection limits were 6 9 10 -3 lM and 9 9 10 -3 lM (S/N = 3), respectively. The nano-copper oxide/GCE could be applied to simultaneously detect guanine and adenine in samples with good anti-interference ability.

show abstract

Rapid and simple determination of adenine and guanine in DNA extract by micellar electrokinetic chromatography with indirect laser-induced fluorescence detection

Cited by 53 publications

References 36 publications

Simultaneous detection of guanine, adenine, thymine and cytosine at choline monolayer supported multiwalled carbon nanotubes film

Simultaneous detection of guanine, adenine, thymine and cytosine at choline monolayer supported multiwalled carbon nanotubes film

Enhanced Indirect Fluorescence Detection of p-Nitrophenol, 2,4-Dinitrophenol and Trinitrophenol by Micellar Electrokinetic Capillary Chromatography with In-column Optical-fiber LED-induced Fluorescence Detection

Electrodeposit nano-copper oxide on glassy carbon electrode for simultaneous detection of guanine and adenine

Contact Info

Product

Resources

About