Rapid and sensitive quantitation of morphine using HPLC with electrochemical detection

Mashayekhi, Siminozar; Ghandforoush-Sattari, Mohammadreza; Hain, Richard

doi:10.1111/j.1365-2710.2008.00933.x

Cited by 16 publications

(10 citation statements)

References 17 publications

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“…The samples were spun down at 3000 rpm for 10 min and the serum was removed and immediately frozen at -201C. The batches of samples were analysed at the Pharmacology, Therapeutic and Toxicology Department, Llandough Hospital using a HPLC with electrochemical detector [5] and a competitive ELISA method [6] for measuring morphine and M6G concentration in the serum samples, respectively. The sensitivity of the HPLC assay was 5.4 nM with a recovery of 93.4%70.01 and the mean intraassay and inter-assay test values for three concentrations were 10.54 and 7.47, respectively [5].…”

Section: Methods Of Morphine and M6g Assaymentioning

confidence: 99%

“…The batches of samples were analysed at the Pharmacology, Therapeutic and Toxicology Department, Llandough Hospital using a HPLC with electrochemical detector [5] and a competitive ELISA method [6] for measuring morphine and M6G concentration in the serum samples, respectively. The sensitivity of the HPLC assay was 5.4 nM with a recovery of 93.4%70.01 and the mean intraassay and inter-assay test values for three concentrations were 10.54 and 7.47, respectively [5]. The sensitivity of the ELISA assay of M6G for serum samples was 1.6 nM while the intra-assay reproducibility and inter-assay test (10 concentrations, n 5 5) were 3.23%71.26 and 9.424%73.1, respectively and the recovery for three concentrations (20, 100 and 200 nM) was 98.2%, 101.9% and 105.3%, respectively [6].…”

Section: Methods Of Morphine and M6g Assaymentioning

confidence: 99%

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Pharmacokinetic and pharmacodynamic study of morphine and morphine 6‐glucuronide after oral and intravenous administration of morphine in children with cancer

Mashayekhi

Ghandforoush-Sattari

Routledge

et al. 2009

Biopharm & Drug Disp

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The aim of this study was to characterize the pharmacokinetics and pharmacodynamics of morphine and morphine 6-glucuronide (M6G) in children with cancer. Serum concentrations of morphine and M6G in children who received single oral or short term continuous intravenous morphine were determined by HPLC and ELISA assays, respectively. The serum C(max) of morphine and M6G after i.v. morphine administration was 560.5 and 309.0 nM and the T(max) was 61 and 65 min, respectively. The elimination half-life was 140.0 and 328.7 min, respectively. After oral administration of morphine, the serum C(max) of morphine and M6G was 408.34 and 256.3 nM and the T(max) was 40.0 and 60 min, respectively. The half-life was 131.0 and 325.8 min, respectively. The side effects were: drowsiness (100%), nausea and/or vomiting (57%), pruritus (28%) and urinary retention (14%). There were no reports of respiratory complications. This study showed that pharmacokinetics factors of morphine and M6G in children were significantly different from adults. Therefore the required dose for children should be different from that of adults and should be based on studies performed on children rather than on studies on adults. Some adverse effects, particularly nausea and pruritus, may be commoner than is usually thought, while others, particularly respiratory problems did not occur.

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Section: Methods Of Morphine and M6g Assaymentioning

confidence: 99%

Section: Methods Of Morphine and M6g Assaymentioning

confidence: 99%

Pharmacokinetic and pharmacodynamic study of morphine and morphine 6‐glucuronide after oral and intravenous administration of morphine in children with cancer

Mashayekhi

Ghandforoush-Sattari

Routledge

et al. 2009

Biopharm & Drug Disp

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“…The mobile phase was sodium phosphate (pH 3.5; 0.10 mol L With these conditions the retention times obtained were: 10.3 min (NZP), 12.5 min (CZP), 13.7 min (FZP) and 18.9 min (DZP). The system suitability parameters: plate counts, resolutions and asymmetry factors, were all in the acceptable range [24].…”

Section: High Performance Liquid Chromatography With Electrochemical mentioning

confidence: 99%

Boron‐Doped Diamond Electrode Coupled to Liquid Chromatography: Application to Simultaneous Determination of Benzodiazepines

et al. 2010

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The applicability of boron-doped diamond (BDD) as a working electrode in an amperometric cell, coupled to HPLC, was demonstrated, for determining benzodiazepines in pharmaceutical preparations. The separation of the benzodiazepines was achieved using a Waters XTerra RP18 column (250 Â 4.6 mm, 5 mm) with a mobile phase sodium phosphate (pH 3.5; 0.10 mol L . The measurements were performed in a system 871 Advanced Bioscan (Metrohm) with a BDD (8000 ppm) adapted to the thin layer mode cell. Stainless steel and platinum wire were used as reference and auxiliary electrodes, respectively. The cell was operated in pulse mode, using À 1.9 V as initial potential. The method presented linearity, repeatability and ruggedness and it represents a novel, alternative electroanalytical method for benzodiazepines determination.

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“…Various analytical methods have been developed for determining morphine and its major metabolites. Typically, the common analytical techniques currently used for monitoring morphine's concentration include GC, 3,4 HPLC, [5][6][7][8][9][10] and their combination with other detection methods. Moreover, there are some other methods can be used as well like capillary electrophoresis, 11,12 chemiluminescence, 13 voltammetric, 14,15 electrochemical, 16 and spectrofluorometric.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometry Method for the Determination of Morphine, Nalbuphine and Naltrexone Drugs in Bulk and Pharmaceutical Formulations

El‐Didamony

Saad

Saleem

2013

J. Chil. Chem. Soc.

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A novel study describes the development and validation of a selective and simple kinetic spectrophotometric method for the determination of some analgesic drugs namely, morphine (MOF), nalbuphine (NAB) and naltrexone (NAT) in bulk and in pharmaceutical formulations. This method was based on the reaction of the drugs under studying with alkaline KMnO 4 to form a water-soluble bluish green colored product with a maximum absorbance at 605 nm. Although, the determination of MOF, NAB and NAT drugs by rate constant, fixed-concentration and fixed time methods were feasible with the calibration equations obtained, the fixed time method has been found to be more applicable. The fixed-time method is adopted for constructing the calibration curves, which were found to be linear over the concentration ranges of 4 -18 mg mL -1 MOF, 2 -20 mg mL -1 NAB and 2 -16 mg mL -1 NAT. Different experimental parameters which can affect the development and stability of the color were carefully studied and optimized. The fixed time method of 20 min was further applied to pharmaceutical formulations of each drug and the percentage recoveries were 99.85±0.049 to 102.27±0.024. Statistical comparisons of the results with the reference methods show the excellent agreement and indicate no significant difference in accuracy and precision.

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Rapid and sensitive quantitation of morphine using HPLC with electrochemical detection

Abstract: This method for morphine in small biological samples is easy, sensitive and reproducible with low cross-reactivity.

Cited by 16 publications

References 17 publications

Pharmacokinetic and pharmacodynamic study of morphine and morphine 6‐glucuronide after oral and intravenous administration of morphine in children with cancer

Pharmacokinetic and pharmacodynamic study of morphine and morphine 6‐glucuronide after oral and intravenous administration of morphine in children with cancer

Boron‐Doped Diamond Electrode Coupled to Liquid Chromatography: Application to Simultaneous Determination of Benzodiazepines

Kinetic Spectrophotometry Method for the Determination of Morphine, Nalbuphine and Naltrexone Drugs in Bulk and Pharmaceutical Formulations

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