Rapid analysis of malachite green and leucomalachite green in fish muscles with surface-enhanced resonance Raman scattering

Zhang, Yuanyuan; Yu, Wei; Lu, Peng; Lai, Keqiang; Rasco, Barbara; Huang, Yiqun

doi:10.1016/j.foodchem.2014.07.129

Cited by 129 publications

(81 citation statements)

References 27 publications

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“…A pair of well-defined oxidation and reduction peaks was observed at the bare GCE (Fig. 4a, curve a); this is due to the high electron transfer between Fe(CN) 6 4 − / 3 − solution and the electrode. Further (Fig.…”

Section: Characterization Of the Immunosensor Assemblymentioning

confidence: 89%

“…Incubation with LMG solution at a different concentration, the peak current of the redox couple was further reduced. It shows that the Ab-LMG complex layer blocked the electron communication between the redox couple Fe(CN) 6 4−/3− and the electrode. Electrochemical impedance spectroscopy is a highly effective technique to investigate the electron transfer properties of the modified electrodes.…”

Section: Characterization Of the Immunosensor Assemblymentioning

confidence: 99%

“…All electrochemical measurements were carried out in 0.1 mol/L PBS solution containing 0.1 mol/L KCl and 5 mmol/L Fe(CN) 6 4−/3− (1:1) as the electrochemical active probe [22]. The cyclic voltammetric scan (−0.3~0.6 V, 50 mV/s) and EIS investigation (0.1~10,000 Hz) were applied to characterize the surface change in the modification process.…”

Section: Electrochemical Measurementmentioning

confidence: 99%

“…A report prepared by the Water Research Centre for the Department of the Environment, Transport, and the Regions of the United Kingdom recommended an annual average environmental quality standard of 500 ng/L LMG and MG for the protection of freshwater aquatic life [3]. Numerous methods have been proposed for the detection of MG and LMG in a variety of matrices, including enzyme-linked immunosorbent assay (ELISA) [4,5], surface-enhanced resonance Raman scattering (SERS) [6], liquid chromatography-tandem mass spectrometry (LC-MS/MS) [2], fluorometry method [7], and so on [8]. Besides this, development of a rapid and highly sensitive method for MG and LMG analyses in fish farms is always required in order to achieve on-site supervision.…”

Section: Introductionmentioning

confidence: 99%

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A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

Zhu

Pang

et al. 2016

Anal Bioanal Chem

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Application of malachite green (MG) and leucomalachite green (LMG) in fish farm water causes an environmental problem. This study proposes for the first time a sensitive and convenient electrochemical impedance spectroscopy (EIS) method for determining MG and LMG by a bovine serum albumin-decorated gold nanocluster (BSA-AuNC)/antibody composite film-based immunosensor. In order to improve the analytical performance, the glassy carbon electrode (GCE) was modified with 1, 4-phenylenediamine to form a stable layer, and then, BSA-AuNCs were covalently bound to the GCE. An adequate quantity of the polyclonal antibody of LMG was immobilized onto the surface of the BSA-AuNCs by the chemical reaction of EDC/NHS. The sensors can respond to the specific target based on specific covalent bonding. The experimental parameters, such as the pH, incubating concentration, and time, have been investigated and optimized. The calibration curve for LMG was linear in the range of 0.1~10.0 ng/mL with the limit of detection (LOD) 0.03 ng/mL. Furthermore, the sum of MG and LMG was detected in fish farm water by MG reduction. The recovery was between 89.7 % and 99.2 % in spiked samples. The EC sensor method was also compared with the ELISA method and validated by the LC-MS/MS method, which proves its great promise as a field instrument for the rapid monitoring of MG and LMG pollution. Graphical abstract 1, 4-Phenylenediamine and BSA-AuNC/antibody-decorated glassy carbon electrodes have been used for the impedimetric detection of the sum of malachite green and leucomalachite green via specific immuno-binding.

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Section: Characterization Of the Immunosensor Assemblymentioning

confidence: 89%

Section: Characterization Of the Immunosensor Assemblymentioning

confidence: 99%

Section: Electrochemical Measurementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

Zhu

Pang

et al. 2016

Anal Bioanal Chem

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“…Direct analysis of MG, CV, and MB residues in fish extracts have been studied by surface-enhanced Raman scattering (SERS) as well. [206][207][208][209] Sorbent and liquid micro-extraction techniques were described in greater detail in Chapter 2.…”

Section: Other Notable Analytical Proceduresmentioning

confidence: 99%