Rapid analysis of amphetamines in blood using head space-solid phase microextraction and selected ion monitoring

Nagasawa, Nobuyuki; Yashiki, Mikio; Iwasaki, Yoshinobu; Hara, Kenji; Kojima, Tohru

doi:10.1016/0379-0738(95)01874-3

Cited by 84 publications

(31 citation statements)

References 9 publications

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“…The analysis for the determination of the DIBderivatives of MP and its metabolite AP in plasma by HPLC-FL showed higher sensitivity than other reported HPLC and GC methods (Cheung et al, 1997;Nagasawa et al, 1996;Gjerde et al, 1993;Hara et al, 1997;Bogusz et al, 1997). In these methods, the reported LOD for AP and MP ranged from 1 to 100 ng/mL using plasma sample volume not less than 0.5 mL.…”

Section: Determination Of Ap and Mp In Abusers' Plasma And Hair Samplesmentioning

confidence: 89%

“…In hair samples, AP and MP could also be detected in very low concentrations. al., , Jonsson et al, 1996Herraez-Hernandez et al, 1998), in addition to plasma (Cheung et al, 1997;Nakashima et al, 1990;Nagasawa et al, 1996;Gjerde et al, 1993;Hara et al, 1997;Bogusz et al, 1997) and hair (Nakahara et al, 1993;Slawson et al, 1996;Takayama et al, 1997), have been detailed.Recently, we reported highly sensitive HPLC methods with fluorescence (FL) detection for the determination of AP and MP in urine (Al-Dirbashi et al, 1998a,b, 2000a and hair samples (Al-Dirbashi et al, 1999, 2000b obtained from MP abusers. In these methods, AP and MP were determined after their derivatization with the fluorescent reagent, 4-(4,5-diphenyl-1H-imidazole-2-yl)benzoyl chloride (DIB-Cl) (Nakashima et al, 1995).…”

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confidence: 97%

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Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC‐FL

Nakashima

Kaddoumi

Ishida

et al. 2003

Biomedical Chromatography

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This paper describes highly sensitive HPLC methods for the determination of amphetamine (AP) and methamphetamine (MP) in abusers' plasma and hair samples. AP and MP were derivatized with the fluorescent reagent, DIB-Cl, to yield a highly fluorescent DIB-derivatives of AP and MP, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 and 430 nm, respectively. The separation was achieved on an ODS column with isocratic mobile phases composed of acetoniltrile and citrate buffer (55:45, v/v) for plasma samples and of acetonitrile-methanol-citrate buffer (45:20:37.5, v/v/v) for hair samples. The limits of detection were less than 0.87 ng/mL and 0.12 ng/mg in plasma and hair samples, respectively, for both AP and MP. The methods were then applied to the determination of MP and its metabolite AP in plasma obtained from two cases of illegally ingested MP and in one of the cases' hair received later. Case I was treated with dialysis; samples before and after dialysis were analyzed by the described method. After dialysis for 5 h, the total plasma levels of AP and MP decreased from 720 to 190 ng/mL. For case II, MP and AP levels were monitored for 3 days after digestion. Total plasma levels decreased from 57 ng/mL in the day of digestion to 11 ng/mL after 3 days. In hair samples, AP and MP could also be detected in very low concentrations.

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Section: Determination Of Ap and Mp In Abusers' Plasma And Hair Samplesmentioning

confidence: 89%

mentioning

confidence: 97%

Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC‐FL

Nakashima

Kaddoumi

Ishida

et al. 2003

Biomedical Chromatography

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“…Determination of MP and its metabolites per se or as enantiomers in biological samples has been extensively investigated using various analytical techniques Hughes et al, 1991;Wolf et al, 1990;Cody and Schwarzhoff, 1993;Ermin et al, 1988;Aoki et al, 1996;Dasgupta et al, 1993;Gjerda et al, 1993;Platoff et al, 1992;Reimer et al, 1993;Yashiki et al, 1995;Nagasawa et al, 1996;Falco et al, 1995;Legua et al, 1995;Helmlin and Brenneisen, 1992;Nakahara and Takeda, 1988;Tatsuno et al, 1996;Hayakawa et al, 1993). Recently, we have been aiming at developing selective and sensitive analytical procedures for the determination of these compounds in biological samples (Nakashima et al, 1990(Nakashima et al, , 1991(Nakashima et al, , 1992Al-Dirbashi et al, 1997;Kuroda et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Al-Dirbashi

Kuroda

Wada

et al. 2000

Biomed. Chromatogr.

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Achiral and chiral semi-micro column high-performance liquid chromatographic methods with fluorescence detection to determine methamphetamine and amphetamine in human hair are described. These compounds were extracted into 5% trifluoroacetic acid (TFA) in methanol, derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride and separated either on a 250 x 1.5 mm i.d. octadecyl-silane (ODS) or a 150 x 2 mm i.d. OD-RH column. Linear calibration curves extending over a wide range of concentration that covers the practical samples were obtained for amphetamine, methamphetamine and their enantiomers (r = 0.999). Resolution values for amphetamine and methamphetamine enantiomers were 3.4 and 1.1, respectively. Intra- and inter-day variations of both the methods were not larger than 8.9% expressed as relative standard deviations (n >/= 5). The limits of detection at a signal-to-noise ratio of 3 obtained by both the methods were in the range of 1.0-4.7 fmol/5 microL injection with the achiral method being more sensitive. Abusers' hair samples were analyzed by the two methods and only the S(+)-enantiomers were found in eight Japanese abusers' hair samples. The achiral method was used to study the concentrations of these compounds in single black and white hair strands of abusers.

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“…Short-chain aliphatic amines are intermediates in chemical and pharmaceutical industries [1], and present in environmental samples, biological fluids, and industrial waste materials, often at trace level [2,3]. Besides hygienic problems due to their unpleasant smell, these compounds have been considered as tumor-inducing agents [4] and may be hazardous to human health as they are sensitizers and irritants to the skin, eye, mucous membranes, and respiratory tract [5 -7].…”

Section: Introductionmentioning

confidence: 99%

Fast field analysis of short‐chain aliphatic amines in water using solid‐phase microextraction and a portable gas chromatograph

Lin

Deng²,

Zhang

2008

J of Separation Science

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A novel method is firstly presented for field and rapid analysis of short-chain aliphatic amines in water as their pentafluorobenzaldehyde (PFBAY) derivative using solid-phase microextraction (SPME) and portable GC. In the proposed method, short-chain aliphatic amines in water rapidly reacted with PFBAY, and then were headspace extracted and concentrated by SPME. The formed amines derivatives were analyzed by portable GC. The SPME parameters of fiber selection, extraction temperature, extraction time, and stirring rate were studied. The method validations including LOD, recovery, precision, and linearity were studied. It was found that the proposed method required the whole analysis time 22 min, and provided low LOD of 1.2-4.6 ng/mL, good recovery of 91-106%, good precision of RSD value 3.5-9.3%, and linear range 20.0-500 ng/mL (r(2) >0.99). The obtained results demonstrated that the SPME-portable GC is a simple, rapid, and efficient method for the field analysis of short-chain aliphatic amines. Finally, the proposed method was further applied to the quantification of ethylamine, propylamine, and butylamine in environmental water.

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Rapid analysis of amphetamines in blood using head space-solid phase microextraction and selected ion monitoring

Cited by 84 publications

References 9 publications

Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC‐FL

Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC‐FL

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Fast field analysis of short‐chain aliphatic amines in water using solid‐phase microextraction and a portable gas chromatograph

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