Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC‐FL

Abstract

This paper describes highly sensitive HPLC methods for the determination of amphetamine (AP) and methamphetamine (MP) in abusers' plasma and hair samples. AP and MP were derivatized with the fluorescent reagent, DIB-Cl, to yield a highly fluorescent DIB-derivatives of AP and MP, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 and 430 nm, respectively. The separation was achieved on an ODS column with isocratic mobile phases composed of acetoniltrile and citrate buffer (55:45, v/v) for plasma samples and of acetonitrile-methanol-citrate buffer (45:20:37.5, v/v/v) for hair samples. The limits of detection were less than 0.87 ng/mL and 0.12 ng/mg in plasma and hair samples, respectively, for both AP and MP. The methods were then applied to the determination of MP and its metabolite AP in plasma obtained from two cases of illegally ingested MP and in one of the cases' hair received later. Case I was treated with dialysis; samples before and after dialysis were analyzed by the described method. After dialysis for 5 h, the total plasma levels of AP and MP decreased from 720 to 190 ng/mL. For case II, MP and AP levels were monitored for 3 days after digestion. Total plasma levels decreased from 57 ng/mL in the day of digestion to 11 ng/mL after 3 days. In hair samples, AP and MP could also be detected in very low concentrations.

Potentiation of N-methyl-d-aspartate receptor-mediated neuronal injury during methamphetamine withdrawal in vitro requires co-activation of IP3 receptors

“…Concentrations of METH employed were chosen to reflect the range of concentrations observed in both plasma and brain of rats following a single i.v. injection of METH (Melega et al 1995), as well as, plasma and hair samples of humans who had ingested METH for recreational purposes (Nakashima et al 2003). Additional cultures were exposed to NMDA (5 μM; Sigma; St. Louis, MO, USA) at the end of the withdrawal period and were also labeled with PI.…”

Development and Application of Sensitive Methods with Luminescence Detections for Determination of Biologically Active Compounds

“…In hair samples, AP and MP could also be detected in very low concentrations. 27) Recently, ecstasy tablets containing other drugs such as MDA, MP, AP, caffeine, aspirin, paracetamol and ketamine were found. Taking these tablets may cause unexpected adverse effects.…”

Analytical Studies on the Development of High-Performance Liquid Chromatographic Methods with Fluorescence or Chemiluminescence Detections and Their Practical Applications

“…When these limitations were overcome, the determination of MP and AP in human plasma and hair samples by an HPLC-FL method with DIB-Cl could be performed. 47 The sample sizes used for plasma and hair were 100 mL and 1 mg, respectively. As an application, MP and AP in plasma and hair obtained from two cases of illegally ingested MP were determined.…”