RAPD marker assessment of self-pollinated inbreeding methods for common bean segregant populations

Luiza, Ana; Castanheira, Monteiro; Santos, João Bosco dos

doi:10.12702/1984-7033.v04n01a01

Cited by 3 publications

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This variability is a function of the genetic diversity of the parents used in the crosses, as well as of the methods applied in the segregating populations (Castanheira 2004). Comparisons among the methods of conducting variable populations in common bean are still scanty and focus mainly on the trait grain yield (Raposo et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Efficiency of methods for conducting segregating populations in the breeding of common beans for protein quality

Silva¹,

Melo²,

Melo³

et al. 2008

CBAB

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Efficiency of methods for conducting segregating populations in the breeding of common beans for protein quality

Silva¹,

Melo²,

Melo³

et al. 2008

CBAB

View full text Add to dashboard Cite

show abstract

Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora

Marraccini¹,

Vinecky²,

Alves³

et al. 2012

Journal of Experimental Botany

View full text Add to dashboard Cite

The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.

show abstract

Eficiência de métodos de melhoramento para teor de fibra e produtividade de grãos em progênies de feijoeiro comum

Silva

Melo

et al. 2013

Bragantia

View full text Add to dashboard Cite

O objetivo deste estudo foi comparar métodos de melhoramento quanto à variabilidade genética e à eficiência na obtenção de progênies superiores para teor de fibra bruta e produtividade de grãos em feijoeiro comum. As progênies foram obtidas do cruzamento, em 2003, entre as linhagens carioca CNFC 7812 e CNFC 7829. A população segregante foi conduzida por bulk, bulk dentro de F2 e SSD até geração F7. Em 2008 foram amostradas aleatoriamente 64 progênies de cada método para avaliação em Goiás e Paraná. Foram obtidas as estimativas dos componentes de variância, parâmetros genéticos e fenotípicos, média e índices de geração de progênies superiores. As progênies obtidas pelos métodos de bulk e SSD apresentaram diferenças significativas para produtividade e teor de fibra bruta e maior variância genética. O bulk dentro de F2 apresentou os maiores índices de geração de progênies superiores e maiores médias para os dois caracteres, sendo recomendado para os programas de melhoramento de feijoeiro comum.

show abstract

RAPD marker assessment of self-pollinated inbreeding methods for common bean segregant populations

Abstract: ABSTRACT

Cited by 3 publications

References 6 publications

Efficiency of methods for conducting segregating populations in the breeding of common beans for protein quality

Efficiency of methods for conducting segregating populations in the breeding of common beans for protein quality

Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora

Eficiência de métodos de melhoramento para teor de fibra e produtividade de grãos em progênies de feijoeiro comum

Contact Info

Product

Resources

About