RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey

Akbulut, Mustafa; Erċışlı, Sezai; Karlıdağ, Hüseyin

doi:10.4238/vol8-3gmr634

Cited by 13 publications

(9 citation statements)

References 14 publications

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“…This study is the first attempt to characterize the figs grown in Palestine using a combination of the PCR-based RAPD technique and morphological characterization. At the molecular level, our results ( Tables 1, 2) and comparable studies in the literature presented high polymorphism ratio (70.2% in 9 RAPD primers) among fig genotypes grown in the Mediterranean countries which commonly ranged between 39% -81% within the same marker (39% in 12 RAPD primers [14]; 67% in 7 RAPD primers [19]; 72% in 6 RAPD primers [10]; 70% in 13 RAPD primers [30]; 77% in 6 RAPD primers [7]; 81% in 7 RAPD primers [21]). Indeed, the high polymorphism that was observed here is indicative of more divergent genotypes, and consequently a potential for success in future selection programs [31].…”

Section: Discussionsupporting

confidence: 85%

Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

Basheer-Salimia

Awad²,

Ward³

2012

AJPS

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Both morphological characters and PCR-based RAPD approaches were used to determine the genetic diversity and relatedness among nine fig genotypes grown at the northern region of the West-Bank, Palestine. Although we tested 28 primers for the RAPD technique, only 9 produced reasonable amplification products. A total of 57 DNA loci were detected in which 70.2% were polymorphic. DNA fragments presented a minimum of 3 and a maximum of 9 polymorphic bands using primers OPT-10 and OPA-18, respectively. Primers exhibited collective resolving power values (Rp) of 18.826. The Mwazi genotype showed the highest genetic distances among all of the other genotypes. Morphologically, considerable variations were found using 41 quantitative and qualitative traits. Adloni could be a very promising genotype for fresh consumption due to its very late maturation period, extended harvesting period, variable fruit size, and easy skin peeling. In addition, 7 genotypes presented firm fruits, which are a very important criterion for exporting purposes. Dendrogram constructed by UPGMA based on RAPD banding patterns appear somewhat contradictory to the morphological descriptors particularly with Swadi and Biadi genotypes (closed genetically and distanced morphologically), which might be attributed to the phenotypic modifications caused by environmental differences across regions. These preliminary results will make a fundamental contribution to further genetic improvement of fig crops for the region.

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Section: Discussionsupporting

confidence: 85%

Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

Basheer-Salimia

Awad²,

Ward³

2012

AJPS

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“…RAPD markers were shown to be useful for distinguishing between Turkish cultivated fig clones, as reported by Cabrita et al (2001). Akbulut et al (2009) (Sadder and Ateyyeh 2006). Compared with the results obtained here, an earlier study showed RAPD markers to be powerful enough to differentiate even between clones of the same fig cultivars (Papadopoulou et al 2002).…”

Section: Discussionsupporting

confidence: 41%

Development of Molecular Tools for Characterization and Genetic Diversity Analysis in Tunisian Fig (Ficus carica) Cultivars

et al. 2010

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Fig, Ficus carica L., is a useful genetic resource for commercial cultivation. In this study, RAPD (60), ISSR (48), RAMPO (63), and SSR (34) markers were compared to detect polymorphism and to establish genetic relationships among Tunisian fig tree cultivars. The statistical procedures conducted on the combined data show considerable genetic diversity, and the tested markers discriminated all fig genotypes studied. The identification key established on the basis of SSR permitted the unambiguous discrimination of cultivars and confirmed the reliability of SSR for fingerprinting fig genotypes. The study findings are discussed in relation to the establishment of a national reference collection that will aid in the conservation of Tunisian fig resources.

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“…Compared with other molecular techniques, RAPD is a simple, fast, efficient, and inexpensive method. Further, it does not require prior knowledge of the sequences of the markers and can produce abundant polymorphic fragments [20,1,3]. Therefore, RAPD has become a powerful and accurate tool for analyzing the genetic relatedness and diversity in figs.…”

Section: Introductionmentioning

confidence: 99%

Molecular polymorphisms in Palestinian Figs (Ficus carica L.) as revealed by Random Amplified Polymorphic DNA (RAPD)

Basheer-Salimia

Awad

Salama

et al. 2012

Journal of Genetic Engineering and Biotechnology

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The genetic diversity of 13 local Palestinian fig genotypes was investigated using RAPD markers. Among the 30 tested primers, 28 revealed various banding patterns and 2 generated no polymorphic bands. In addition, 13 primers (46.4%) produced good amplification products with high intensity and pattern stability. A total of 94 DNA fragments (loci), separated by electrophoresis on agarose gel were detected, ranging in size from 190 to 1300 bp. Of these fragments, 72 (76.6%) were polymorphic and 22 (23.4%) were monomorphic. A minimum of three and a maximum of eight DNA fragments were obtained using (OPH-02 and OPT-10) as well as (OPA-13, OPA-18 and OPY-07) primers respectively. The maximum percentage of polymorphic markers was 100.0 (Z-5, Z-12, and OPT-10) and the minimum was 60.0 (OPH-02). Primers OPY-07 and OPA-13 revealed high collective resolving power (Rp) values with 4.640 and 4.760 respectively and therefore, they were the most useful RAPD primers to assess the genetic diversity in the Palestinian figs. Genetic distance matrix showed an average distance range from 0.186 to 0.559 with a mean of 0.373. Thus, the cultivars tested in this study were characterized by large divergence at the DNA level. To our knowledge, this is the first report using RAPD marker to assess genetic diversity of Palestinian figs.

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RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey

Cited by 13 publications

References 14 publications

Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

Development of Molecular Tools for Characterization and Genetic Diversity Analysis in Tunisian Fig (Ficus carica) Cultivars

Molecular polymorphisms in Palestinian Figs (Ficus carica L.) as revealed by Random Amplified Polymorphic DNA (RAPD)

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