RAPD Assessment for Identification of Clonal Identity and Genetic Stability of in vitro Propagated Chestnut Hybrids

Carvalho, Luísa C.; Goulão, L.; Oliveira, Cristina M.; Gonçalves, José Carlos; Amâncio, Sara

doi:10.1023/b:ticu.0000016482.54896.54

Cited by 64 publications

(46 citation statements)

References 26 publications

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“…The application of OPT06 as a primer could amplify hybrid DNA and can be used for testing hybridity of the cross 366 (D) × 72 (P). These results are similar to those reported in chestnut 27 . They indicated that RAPD markers were successfully used for detecting the material propagated in vitro and the donor plants of chestnut.…”

Section: Discussionsupporting

confidence: 82%

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Thawaro¹,

Te-chato²

2009

ScienceAsia

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ABSTRACT:Randomly amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) were used to assess the hybrid verification and somaclonal variation of the cross 366 (D) × 72 (P) of Elaeis quineensis Jacq. DxP. DNA from mature zygotic embryo (MZE) was isolated and detected by seven primers of RAPD and eight primers of SSR. Half MZEs consisting of coleoptiles regions were cultured on Murashige and Skoog (MS) medium plus various kinds and concentrations of auxins. Variation of somaclone obtained through somatic embryogenesis was detected at the proliferation stage of the culture period. The result revealed that all random primers and the SSR primers tested could amplify parental DNA. Primer OPT06 (for RAPD) and EgCIR1772 (for SSR) provided clear DNA patterns and could be used for verifying the hybridity of the cross 366 (D) × 72 (P). The highest frequency of nodular callus formation at 87.5% was obtained on MS medium supplemented with 2.50 mg/l of 3,6-dichloro-o-anisic acid (dicamba). This was significantly different from other kinds and concentrations of auxins. Somatic embryo line at globular stage derived from each half MZE showed no variation in RAPD banding patterns with primer OPT06. It was concluded that no somaclonal variation occurred in our propagation system by RAPD marker, no major genetic changes were observed, and this augurs well for the propagation system being employed since somaclonal variation may be minimized or absent altogether.

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Section: Discussionsupporting

confidence: 82%

Untitled

Thawaro¹,

Te-chato²

2009

ScienceAsia

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“…Martins et al (2004) reported the genetic stability of micropropagated almond plants. The genetic stability of in vitro propagated Picea mariana was also reported by Isabel et al (1993) and in vitro-adventitious shoots of Pinus taeda (Tang 2001) and chestnut (Carvalho et al 2004). PCR based molecular markers including ISSR are very simple, fast, reliable, highly discriminative and inexpensive.…”

Section: Discussionmentioning

confidence: 84%

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Khateeb

Bahar

Lahham

et al. 2012

Physiol Mol Biol Plants

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Moringa peregrina is an endangered species of Moringaceae. M. peregrina is a multipurpose tree with a wide variety of potential uses including its medicinal activity. In our study, a rapid and efficient micropropagation protocol for M. peregrina has been established. In vitro germinated seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different levels of either 6-benzyladenine (BA) or kinetin (Kin). The maximum shoot proliferation of 6.5 shoots per explant with 100 % shoot proliferation rate was observed on MS medium supplemented with 1.0 mg/l BA. On the other hand, MS medium supplemented with 1 mg/l indole-3-butyric acid (IBA) resulted in the maximum number of roots. Micropropagated plants were successfully acclimatized. Genetic stability of micropropagated plants was assessed using Inter-Simple Sequence Repeat (ISSR). The amplification products were monomorphic in all in vitro grown plants. No polymorphism was detected indicating the genetic integrity of in vitro propagated plants. This micropropagation protocol could be useful for raising genetically uniform plants for plant propagation and commercial cultivation.

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“…RAPD is becoming a widely employed method in the detection of genetic diversity because it has the advantage of being technically simple, quick to perform and requires only small amounts of DNA (Ceasar et al 2010). Many investigators have reported genetic stability of several micropropagated plants, viz., chestnut rootstock hybrid (Carvalho et al 2004), Prunus dulcis (Martins et al 2004), banana (Venkatachalam et al 2007), etc. using RAPD.…”

Section: Assessment Of Genetic Fidelity Of In Vitro Propagated Plantsmentioning

confidence: 99%

In vitro propagation of spine gourd (Momordica dioica Roxb.) and assessment of genetic fidelity of micropropagated plants using RAPD analysis

Kumar

Singh

Prakash

et al. 2012

Physiol Mol Biol Plants

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An efficient protocol for rapid in vitro clonal propagation of spine gourd (Momordica dioica Roxb.) genotype RSR/DR15 (female) and DR/NKB-28 (male) was developed through enhanced axillary shoot proliferation from nodal segments. Maximum shoot proliferation of 6.2 shoots per explant with 100 % shoot regeneration frequency was obtained from the female genotype on Murashige and Skoog's (1962) medium supplemented with 0.9μM N6-benzyladenine (BA) and 200 mg l -1 casein hydrolysate (CH). While from the male genotype the optimum shoot regeneration frequency (86.6 %) and 6.4 shoots per explant was obtained on MS medium supplemented with 2.2μM BA. CH induced vigorous shoots, promoted callus formation, and proved inhibitory for shoot differentiation and shoot length, especially in explants from male genotype. Rooting was optimum on half-strength MS medium (male 92.8 %, female 74.6 %) containing 4.9μM indole-3-butyric acid (IBA). Plantlets were transferred to plastic cups containing a mixture of cocopit and perlite (1:1 ratio) and then to soil after 2-3 weeks. 84 % female and 81 % male regenerated plantlets survived and grew vigorously in the field. Genetic stability of the regenerated plants was assessed using random amplified polymorphic DNA (RAPD). The amplification products were monomorphic in the in vitro propagated plants and similar to those of mother plant. No polymorphism was detected revealing the genetic integrity of in vitro propagated plants. This micropropagation procedure could be useful for raising genetically uniform planting material of known sex for commercial cultivation or build-up of plant material of a specific sex-type.

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RAPD Assessment for Identification of Clonal Identity and Genetic Stability of in vitro Propagated Chestnut Hybrids

Cited by 64 publications

References 26 publications

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Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

In vitro propagation of spine gourd (Momordica dioica Roxb.) and assessment of genetic fidelity of micropropagated plants using RAPD analysis

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