Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Khateeb, Wesam Al; Bahar, Eman; Lahham, Jamil N.; Schroeder, Dana F.; Hussein, Emad I.

doi:10.1007/s12298-012-0149-z

Cited by 43 publications

(29 citation statements)

References 26 publications

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“…The formation of callus and adventitious shoots is influenced by the interactions between endogenous and exogenous plant growth regulators (Guo et al 2016). Multiple studies have shown that 6-BA influences adventitious shoot differentiation more than do other cytokinins (Al Khateeb et al 2013;Lee and Pijut 2017;Zhang et al 2017), but only in the condition of rational configuration of cytokinin and auxin, there is a high frequency induction in explants.…”

Section: Effects Of Plant Growth Regulators On Shoot Inductionmentioning

confidence: 99%

Development of an efficient regeneration system for the precious and fast-growing timber tree <i>Toona ciliata</i>

Shang

Zhou

et al. 2018

Plant Biotechnology

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Toona ciliata (Chinese mahogany) is an important timber species and secondary protected plant due to excessive exploitation in China. Here we developed a robust and efficient regeneration system for adventitious shoot induction using hypocotyl explants of T. ciliata. To facilitate plant growth, different regulators were added to MurashigeSkoog (MS) medium (0.5 mg/l 6-BA, 1.0 mg/l KT and 0.1 mg/l IBA). A regeneration frequency of 58.67% with four shoots per explant was achieved by horizontal setting of hypocotyls on MS medium and following a 20-day seeding period. MS medium supplemented with 0.3 mg/l 6-BA and 0.2 mg/l NAA was optimal for shoot multiplication and elongation, with a multiplication coefficient of 3.06. A rooting frequency of 93.33% was achieved using the half-strength MS containing 0.1 mg/l NAA. After acclimatization, plantlets were transplanted to sterilized nutrient soil containing a 2 : 1 ratio of vermiculate with 90% survival frequency. Thus, the regeneration system developed in this study would be useful for genetic transformation and other biotechnology endeavours in T. ciliata.

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Section: Effects Of Plant Growth Regulators On Shoot Inductionmentioning

confidence: 99%

Development of an efficient regeneration system for the precious and fast-growing timber tree <i>Toona ciliata</i>

Shang

Zhou

et al. 2018

Plant Biotechnology

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“…The fruits are elongate capsules, with a beak, glabrous and slightly narrowed between the seeds. The seeds are globose to ovoid or trigonous [10,19,20]. Plantations of M. peregrina have been assessed as quite promising, with growth reasonably rapid and cultivation easy [15].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characterization and thermal behavior of biodiesel and biodiesel–diesel blends derived from crude Moringa peregrina seed oil

Salaheldeen

Aroua

Mariod

et al. 2015

Energy Conversion and Management

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a b s t r a c tMoringaceae is a monogeneric family with a single genus i.e. Moringa. This family includes 13 species. All these species are known as medicinal, nutritional and water purification agents. This study reports, for the first time, on characterization of the biodiesel derived from crude Moringa peregrina seed oil and its blends with diesel. The crude oil was converted to biodiesel by the transesterification reaction, catalyzed by potassium hydroxide. High ester content (97.79%) was obtained. M. peregrina biodiesel exhibited high oxidative stability (24.48 h). Moreover, the major fuel properties of M. peregrina biodiesel conformed to the ASTM D6751 standards. However, kinematic viscosity (4.6758 mm 2 /s), density (876.2 kg/m 3 ) and flash point (156.5°C) were found higher than that of diesel fuel. In addition, the calorific value of M. peregrina biodiesel (40.119 MJ/kg) was lower than the diesel fuel. The fuel properties of M. peregrina biodiesel were enhanced significantly by blending with diesel fuel. In conclusion, M. peregrina is a suitable feedstock for sustainable production of biodiesel only blended up to 20% with diesel fuel, considering the edibility of all other parts of this tree.

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“…This finding was also corroborated by Bonyanpour and Khosh-Khui (2013) who reported that contrary results obtained in shoot induction response was caused by differences in cultivar and explant type. Different results were also obtained from other plant species as well (Khateeb, Bahar, Lahham, Schroeder, & Hussein, 2012;Baskaran, Moyo, & Van Staden, 2013). Idowu, Ibitoye and Ademoyegun (2009) reported that in general explants obtained from different organs vary in their regeneration capabilities and juvenile tissues have higher micropropagation potential than mature tissues.…”

Section: Callusmentioning

confidence: 98%

Pomegranate Micropropagation, Callogenesis and Genetic Integrity Assessment Using Simple Sequence Repeat Markers

Stimela¹,

Kasili²,

Mamati³

2018

JAS

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In recent years, the awareness of pomegranate health benefits has grown exponentially; nonetheless the existing propagation methods remain a challenge to supply adequate suitable planting materials needed for commercial production. Micropropagation can lead to mass production of plantlets and callus-mediated in vitro regeneration can open avenues for the use of genetic engineering to improve this crop. The aim of this study was to evaluate appropriate conditions for pomegranate micropropagation, callogenesis and use Simple Sequence Repeat markers to screen for somaclonal variation. Cytokinins (Benzylaminopurine, Kinetin and Thiadiazol-5ylurea) were tested for shoot induction from nodal explants while auxins (1-Naphthaleneacetic acid, Indole-3-butyric acid and Indole-3-acetic acid) were tested for root induction of in vitro regenerated shoots. 1-Naphthaleneacetic acid combined with Benzylaminopurine was assessed for their ability to induce callus from cotyledon and leaf explants. Genetic integrity between mother plant, callus and in vitro regenerated shoots were assessed using eight Simple Sequence Repeat markers. Maximum number of shoots and leaves were obtained on full strength Murashige and Skoog media with 6.9 µM kinetin. The highest number of roots was achieved on half strength Murashige and Skoog media with 4.9 µM Indole-3-butyric acid and the longest root was got on half strength Murashige and Skoog media with 5.3 µM Indole-3-acetic acid. Leaves and cotyledons demonstrated to be potential explants for callus formation at all hormonal combination levels tested. Eight out of 13 amplified alleles were polymorphic. A wider genetic variation was found with similarity coefficient range of 0.46-0.92. More somaclonal variation was in regenerated shoots compared to callus.

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Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Cited by 43 publications

References 26 publications

Development of an efficient regeneration system for the precious and fast-growing timber tree <i>Toona ciliata</i>

Development of an efficient regeneration system for the precious and fast-growing timber tree <i>Toona ciliata</i>

Physicochemical characterization and thermal behavior of biodiesel and biodiesel–diesel blends derived from crude Moringa peregrina seed oil

Pomegranate Micropropagation, Callogenesis and Genetic Integrity Assessment Using Simple Sequence Repeat Markers

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