RAPD and SCAR Markers Linked to a Gene Conferring Resistance to Angular Leaf Spot in Common Bean

Sietsche, S.; Borém, Aluízio; Carvalho, G. M. C.; Rocha, R. C.; Paula, T.J. de; Barros, Everaldo Gonçalves de; Moreira, Maurílio Alves

doi:10.1046/j.1439-0434.2000.00479.x

Cited by 48 publications

(43 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The resistance present in these genotypes in crosses with susceptible cultivar Rudá was shown to be monogenic and dominant (Caixeta et al, 2003;Carvalho et al, 1998;Ferreira et al, 2000;Nietsche et al, 2000;Sartorato et al, 2000). However, using allelism tests with these same cultivars Caixeta et al (2005) demonstrated that the complexity involving the resistance loci in these sources is greater than previously thought.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Angular Leaf Spot Resistance Gene Present in Common Bean Cultivar Ouro Negro

Sanglard¹,

Ribeiro²,

Balbi³

et al. 2013

JAS

View full text Add to dashboard Cite

Angular leaf spot (ALS), incited by the fungus Pseudocercospora griseola, is a major disease affecting common bean. Important resistance sources have been identified and characterized: AND 277, BAT 332, Cornell 49-242, MAR-2, and Mexico 54. P. griseola presents high physiological variability. The control of this kind of pathogen requires continuous evaluation, including monitoring and characterization of pathogenic variability, and eventual germplasm introgression of genes in adapted cultivars. Cultivar Ouro Negro is currently being used as an ALS resistance source in Brazil. This work aimed to characterize the ALS resistance gene present in cultivar Ouro Negro in relation to the other five resistance sources. These cultivars were crossed with 'Ouro Negro' (allelism tests) and with susceptible cv. Rudá (inheritance studies) and their segregation patterns were evaluated. The inheritance studies and the allelism tests indicate that 'Ouro Negro' harbors at least one resistance locus which is distinct from those present in the other resistance sources.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of the Angular Leaf Spot Resistance Gene Present in Common Bean Cultivar Ouro Negro

Sanglard¹,

Ribeiro²,

Balbi³

et al. 2013

JAS

View full text Add to dashboard Cite

show abstract

“…Polymorphic fragments could also be sequenced, with the aim of identifying potential genes that are variable throughout populations of the species. Sequenced fragments could be converted to co-dominant markers, such as sequenced characterized amplified regions (SCAR), with high specificity (Nietsche et al, 2000).…”

Section: Presence Of Monomorphic Fragmentsmentioning

confidence: 99%

Genetic analysis of Apuleia leiocarpa as revealed by random amplified polymorphic DNA markers: prospects for population genetic studies

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Apuleia leiocarpa (Vogel) J.F. MacBride is a hardwood species native to South America, which is at serious risk of extinction. Therefore, it is of prime importance to examine the genetic diversity of this species, information required for developing conservation, sustainable management, and breeding strategies. Although scarcely used in recent years, random amplified polymorphic DNA markers are useful resources for the analysis of genetic diversity and structure of tree species. This study represents the first genetic analysis based on DNA markers in A. leiocarpa that aimed to investigate the levels of polymorphism and to select markers for the precise characterization of its genetic structure. We adapted the original DNA extraction protocol based on cetyltrimethyl ammonium bromide, and describe a simple procedure that can be used to obtain high-quality samples from leaf tissues of this tree. Eighteen primers were selected, revealing 92 bands, from which 75 were polymorphic and 61 were sufficient to represent the overall genetic structure of the population without compromising the precision of the analysis. Some fragments were conserved among individuals, which can be sequenced and used to analyze nucleotide diversity parameters through a wider set of A. leiocarpa individuals and populations. The individuals were separated into 11 distinct groups with variable levels of genetic diversity, which is important for selecting desirable genotypes and for the development of a conservation and sustainable management program. Our results are of prime importance for further investigations concerning the genetic characterization of this important, but vulnerable species.

show abstract

“…For example, Nietsche et al (2000) found two RAPD markers, OPN02 890 and OPE04 650, linked to resistance to angular leaf spot in the cultivar 'Cornell 49-242', located at 3.2 and 12.5 cM from the locus, respectively . Ferreira et.…”

Section: Identification Of Rapd and Ssr Markersmentioning

confidence: 99%

“…Large variability has been found in the population of the pathogen (Sartorato and Rava, 1994;Paula-Jr and Zambolim, 1998;Nietsche et al, 2000;Sartorato, 2002). However, most cultivars currently used in Brazil, and consequently the disease-causing races, are of Mesoamerican origin.…”

Section: Introductionmentioning

confidence: 99%

Identification of SSR and RAPD markers linked to a resistance allele for angular leaf spot in the common bean (Phaseolus vulgaris) line ESAL 550

2003

View full text Add to dashboard Cite

The objective of this study was to identify RAPD and SSR markers associated with a resistant allele for angular leaf spot (Phaeoisariopsis griseola) from the line 'ESAL 550', derived from the Andean 'Jalo EEP 558' cultivar, to assist selection of resistant genotypes. The resistant line 'ESAL 550' and the susceptible cultivar 'Carioca MG' were crossed to generate F 1 and F 2 populations. One hundred and twenty F 2:3 families were evaluated. The DNA of the 12 most resistant families was bulked and the same was done with the DNA of the 10 most susceptible, generating two contrasting bulks. One RAPD and one SSR marker was found to be linked in coupling phase to the resistant allele. The SSR marker was amplified by the primer PV-atct001 282C , and its distance from the resistant allele was 7.6 cM. This is the most useful marker for indirect selection of resistant plants in segregating populations. The RAPD marker was amplified by the primer OPP07 857C linked in coupling phase to the resistant allele, and distant 24.4 cM. Therefore, this RAPD marker is not so useful in assisting selection because it is too far from the resistant allele.

show abstract

RAPD and SCAR Markers Linked to a Gene Conferring Resistance to Angular Leaf Spot in Common Bean

Cited by 48 publications

References 13 publications

Characterization of the Angular Leaf Spot Resistance Gene Present in Common Bean Cultivar Ouro Negro

Characterization of the Angular Leaf Spot Resistance Gene Present in Common Bean Cultivar Ouro Negro

Genetic analysis of Apuleia leiocarpa as revealed by random amplified polymorphic DNA markers: prospects for population genetic studies

Identification of SSR and RAPD markers linked to a resistance allele for angular leaf spot in the common bean (Phaseolus vulgaris) line ESAL 550

Contact Info

Product

Resources

About