RAPD and isoenzyme analysis of genetic variability in three allied species of catfish (Siluriformes: Pimelodidae) from the Tibagi River, Brazil

Almeida, Fernanda Simões de; Fungaro, Maria Helena Pelegrinelli; Sodré, Leda Maria Koelblinger

doi:10.1017/s0952836901000103

Cited by 45 publications

(36 citation statements)

References 14 publications

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“…annectans (n=203) and Eg. pleosticta (n=269) was performed as in Freiria et al (2012), while DNA from males of Eulaema cingulata (n=319), Euglossa iopoecila (n=271) and Euglossa truncata (n=42) was obtained according to Almeida et al (2001), with minor modifications, described as follows: parts of the body (legs or thorax) were placed in micro-centrifuge tubes containing 300 μL extraction buffer (500 mm Tris-HCl, 20 mm EDTA, 10 mm NaCl, pH 8.0 and 1 % SDS) and 5 μL K proteinase (20 μg/μL). Using small scissors, the body parts of bees were cut, held in a water bath at 63°C for two hours and then subjected to phenolchloroform washes.…”

Section: Dna Extraction and Molecular Analysismentioning

confidence: 99%

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

et al. 2015

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-The incidence of diploid males among 1457 individuals of different populations of six euglossine bee species (Eufriesea violacea , Eulaema cingulata , Euglossa annectans , Euglossa iopoecila , Euglossa pleosticta and Euglossa truncata ) was investigated with microsatellite markers. Bees were surveyed in Atlantic Forest fragments showing different sizes and degrees of human interference. Our analyses revealed that, although the frequencies of diploid males remained below 10 % for five out of six species studied, the frequencies of these males in three populations of Eg. annectans were above 15.0 %. For this species, while the average estimate of diploid males was around 11.0 %, the highest percentage (27 %) of such males was found for the population from an island. Our findings indicate that the frequency of diploid males can vary substantially among species and this fact should be taking into consideration in studies and conservation actions involving different euglossine bees. microsatellites / Euglossini / euglossine bees / Euglossa annectans / forest fragments

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Section: Dna Extraction and Molecular Analysismentioning

confidence: 99%

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

et al. 2015

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“…Random amplified polymorphic DNA (RAPD) analysis, a simple and straightforward PCR-based technique (Williams et al, 1990), employs a class of markers increasingly used to evaluate genetic variability and structure in a variety of organisms (Kimberling et al, 1996;Almeida et al, 2001;Allnutt et al, 2003).…”

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confidence: 99%

Genetic structure analysis of Eufriesea violacea (Hymenoptera, Apidae) populations from southern Brazilian Atlantic rainforest remnants

et al. 2005

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Random amplified polymorphic DNA (RAPD) markers were used to analyze the genetic structure of Eufriesea violacea populations in three fragments (85.47, 832.58 and 2800 ha) of Atlantic rainforest located in the north of the Brazilian state of Paraná. A total of twelve primers produced 206 loci, of which 129 were polymorphic (95% criterion). The proportions of polymorphic loci in each population ranged from 57.28% to 59.2%, revealing very similar levels of genetic variability in the groups of bees from each fragment. Unbiased genetic distances between groups ranged from 0.0171 to 0.0284, the smallest genetic distance occurring between bees from the two larger fragments. These results suggest that the E. violacea populations from the three fragments have maintained themselves genetically similar to native populations of this species originally present in northern Paraná.

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“…DNA was isolated from a few milligrams of lyophilized tissue using a protocol developed by Shiozawa et al (1992), with some modifications (Almeida et al 2001). DNA quality and concentration were evaluated via electrophoresis in agarose gels following staining with SYBR Safe (Invitrogen, CA, USA).…”

Section: Sample Collection and Dna Extractionmentioning

confidence: 99%

Population genetic structure of an estuarine and a reef fish species exploited by Brazilian artisanal fishing

Priolli

Bajay²,

Silvano³

et al. 2016

Sci. Mar.

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Summary:In this study, we used microsatellite markers to examine the genetic structures of Centropomus undecimalis (Bloch, 1792) and Epinephelus marginatus (Lowe, 1834) populations collected from artisanal fishing sites along a stretch of coastline in southeastern Brazil. Based on F-statistics, there was no significant genetic differentiation evident in any C. undecimalis samples (F ST =0.012). However, Bayesian clustering, principal component analysis (PCA) and discriminant analysis of principal components (DAPC) results suggested that there were most likely two clusters, with no relation to geographic areas. The bottleneck results showed no significant values and the effective population sizes (N e ) for the two genetically differentiated groups were large and similar. In contrast, for E. marginatus populations, the microsatellite loci showed no population subdivisions. The F ST value was low and non-significant (F ST =0.008), a Bayesian analysis indicated one cluster, and a PCA showed that all samples from different geographical sites shared the same genetic structure. The bottleneck results exhibited significant differences, and a low N e was observed. The results of the genetic study of these two species along the southeastern Brazilian coast suggest that the distinct genetic structure of each species should be taken into account as management units for the conservation of their genetic diversities.Keywords: small-scale fisheries; microsatellites; genetic diversity; common snook; dusky grouper; bottleneck. Estructura genética poblacional de dos especies piscícolas, una de estuario y una de arrecife, explotados por la pesca artesanal brasilera Resumen: En este estudio se han utilizado marcadores microsatélites para examinar la estructura genética de Centropomus undecimalis (Bloch, 1792) y Epinephelus marginatus (Lowe, 1834) en localidades de pesca artesanal localizadas a lo largo de la costa del sudeste de Brasil. Los estadísticos de diferenciación poblacional (F-statisctics) no presentaron diferenciación genética entre las muestras de C. undecimalis (F ST =0.012). Sin embargo, los resultados de los análisis basados en clústeres bayesianos, componentes principales (PCA) y análisis discriminante de componentes principales (DAPC) sugieren la posible presencia de dos clústeres diferenciados genéticamente, pero sin ninguna relación con las áreas geográficas. Los resultados del análisis de cuello de botella poblacional no son significativos con valores de tamaño efectivo poblacional (N e ) elevados y similares entre los dos clústeres. Por el contrario, en E. marginatus, los análisis basados en microsatélites no muestran ningún patrón de subdivisión genética. El valor de F ST es bajo y no significativo (F ST =0.008), el análisis Bayesiano indicia un solo clúster y el PCA determina que todas las muestras de las diferentes localidades geográficas comparten la misma estructura genética. El análisis de cuello de botella muestra diferencias significativas con la observación de una baja N e . Los resultados de los anál...

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RAPD and isoenzyme analysis of genetic variability in three allied species of catfish (Siluriformes: Pimelodidae) from the Tibagi River, Brazil

Cited by 45 publications

References 14 publications

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

Genetic structure analysis of Eufriesea violacea (Hymenoptera, Apidae) populations from southern Brazilian Atlantic rainforest remnants

Population genetic structure of an estuarine and a reef fish species exploited by Brazilian artisanal fishing

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