We have used a pulse-injection method to fix multi-walled carbon nanotubes (MWNTs) onto hydrogen-terminated Si(100) surfaces. Using scanning-tunneling-microscopy (STM), we first tested several kinds of solvents for organic molecules which were pulse-injected onto the H-terminated Si(100) surfaces. Most of the solvent molecules of hexane and chloroform were desorbed from the surfaces after annealing the substrates, which indicates that they are suitable for fixing molecules onto the surface. Then, we fixed MWNTs which were dispersed in hexane and pulse-injected onto the H-terminated surface. An isolated MWNT was observed by STM and was investigated by scanning tunneling spectroscopy (STS). The STS spectra revealed a metallic feature of the particular MWNT.