Rana grylio virus as a vector for foreign gene expression in fish cells

He, Libo; Fei, Ke; Zhang, Qi-Ya

doi:10.1016/j.virusres.2011.08.012

Cited by 17 publications

(21 citation statements)

References 54 publications

(54 reference statements)

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“…We found that RGV could induce apoptosis mediated by mitochondria [25]. RGV has the potential to be used as a viral vector for expression of foreign genes in fish cells [26].…”

mentioning

confidence: 84%

Sequencing and analysis of the complete genome of Rana grylio virus (RGV)

Zhu

et al. 2012

Arch Virol

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Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses.

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“…We found that RGV could induce apoptosis mediated by mitochondria [25]. RGV has the potential to be used as a viral vector for expression of foreign genes in fish cells [26].…”

mentioning

confidence: 84%

Sequencing and analysis of the complete genome of Rana grylio virus (RGV)

Zhu

et al. 2012

Arch Virol

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“…A recombinant RGV ( TK-RGV) containing EGFP gene was constructed in our laboratory (He et al, 2012). UCS cells infection with TK-RGV was performed as described above.…”

Section: Cell Transfection With Pegfp Express Vector and Infection Wimentioning

confidence: 99%

“…Recombinant virus could be used to study viral gene functions and serve as delivery vehicles for biological control of some diseases (Chinchar et al, 2011;He et al, 2013He et al, , 2014. A recombinant RGV, TK-RGV, was constructed (He et al, 2012). The virus can express enhanced green fluorescence protein after infection and replication in cells, and the infection can be directly observed under a fluorescence microscope.…”

Section: Introductionmentioning

confidence: 99%

Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses

et al. 2014

Virus Research

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“…Primer Primer sequence (5 -3 ) P1CAGAAGCTTACTAGGTCCGCCGACG (Hind III) P2 ACGTTCTCGGAGGAGGCCATGCGCATTGTGAATGTAAAGT P3 ACTTTACATTCACAATGCGCATGGCCTCCTCCGAGAACGT P4 AAGCTCGAGTTACAGGAACAGGTGG (XhoI) P5 ATGAACGGAAACTCTCTGCA P6 TCAGACAAAACTCTCCTTAAGATC P7 AACCTCTGAGAAAGCGCAAG P8CCACAACTAGAATGCAGTGAAAA P9 ATGTGCATCCCTACAGTCATAGC P10 TTACCGCACATTTCTAGACACAA P11 ACTAGGTCCGCCGACGAG P12 TTACAGGAACAGGTGGTGGC pGL3-pICP18-RFP-TK (He et al, 2012). The schematic diagram of the recombinant plasmid construction is shown in Fig.…”

Section: Recombinant Plasmid Constructionmentioning

confidence: 99%

“…In brief, the EGFP gene driven by MCP promoter p50 was inserted into TK or DUT locus though homologous recombination (He et al, 2012;Huang et al, 2014). Both TK and DUT played roles in the nucleotide metabolism, and may either directly or indirectly function in the synthesis of dTMP.…”

Section: Introductionmentioning

confidence: 99%