Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses

Ou, Tong; Li, Xiaoying; He, Libo; Zhou, Feng-Jian; Zhang, Qi-Ya

doi:10.1016/j.virusres.2014.04.013

Cited by 18 publications

(15 citation statements)

References 37 publications

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“…Similar results appeared in other research which determined that the isolated Pseudobagrus ussuriensis skin cell line showed differential CPEs after inoculation of SVCV, GCRV and Rana grylio virus (Ou et al 2014). In addition, the virus titers of GCRV and GSIV were significantly inhibited after medication with a low concentration of Mor or Rib.…”

Section: Discussionsupporting

confidence: 89%

“…The epithelioma papulosum cyprinid (EPC) cell line is known to be susceptible to infection with GSIV and SVCV (Koutná et al 2003, Ma et al 2014, and the findings in our study indicated that the grass carp ovary (GCO) cell line is susceptible to infection with GCRV, which is consistent with the findings of a previous study (Ke et al 2013). The GCO cell line is known to be susceptible to viruses such as Cyprinus carpio spring viremia virus, Rana grylio virus, Paralichthys olivaceus lymphocystis virus (Zhang et al 2003, Ou et al 2014 and was used to replicate and propagate part of the virus. The EPC cell line represents a suitable in vitro model to study GSIV morphogenesis and characterize the GSIV replication cycle (Gao et al 2012, Ma et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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In vitro antiviral efficacy of moroxydine hydrochloride and ribavirin against grass carp reovirus and giant salamander iridovirus

Chen²,

Shan³

et al. 2016

Dis. Aquat. Org.

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Moroxydine hydrochloride (Mor) and ribavirin (Rib) have been reported to exhibit multi-antiviral activities against DNA and RNA viruses, but their antiviral activities and pharmacologies have seldom been studied in aquaculture. This paper has selected 3 aquatic viruses including a double-stranded RNA virus (grass carp reovirus, GCRV), a single-stranded RNA virus (spring viraemia of carp virus, SVCV) and a DNA virus (giant salamander iridovirus, GSIV) for antiviral testing. The results showed that Mor and Rib can effectively control the infection of GCRV and GSIV in respective host cells. Further study was undertaken to explore the antivirus efficiencies and pharmacological mechanisms of Mor and Rib on GCRV and GSIV in vitro. Briefly, compounds showed over 50%

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

In vitro antiviral efficacy of moroxydine hydrochloride and ribavirin against grass carp reovirus and giant salamander iridovirus

Chen²,

Shan³

et al. 2016

Dis. Aquat. Org.

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“…As the largest one of bagrid catfish species, it has been recently added into aquaculture in China because of its delicious meal and better production performance (Ou et al 2014;Wang et al 2013b). Field surveys and aquaculture practices have observed significant sexual dimorphism in growth rate and body size, in which males grow faster than females, and result in an ultimate threefold size difference.…”

Section: Introductionmentioning

confidence: 99%

Identification of Sex-Specific Markers Reveals Male Heterogametic Sex Determination in Pseudobagrus ussuriensis

Pan

Zhou

et al. 2015

Mar Biotechnol

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Comprehending sex determination mechanism is a first step for developing sex control breeding biotechnologies in fish. Pseudobagrus ussuriensis, one of bagrid catfishes in Bagridae, had been observed to have about threefold size dimorphism between males and females, but its sex determination mechanism had been unknown. In this study, we firstly used the amplified fragment length polymorphism (AFLP)-based screening approach to isolate a male-specific DNA fragment and thereby identified a 10,569 bp of male-specific sequence and a 10,365 bp of female-related sequence by genome walking in the bagrid catfish, in which a substantial genetic differentiation with 96.35 % nucleotide identity was revealed between them. Subsequently, a high differentiating region of 650 bp with only 70.26 % nucleotide identity was found from the corresponding two sequences, and three primer pairs of male-specific marker, male and female-shared marker with different length products in male and female genomes, and female-related marker were designed. Significantly, when these markers were used to identify genetic sex of the bagrid catfish, only male individuals was detected to amplify the male-specific marker fragment, and female-related marker was discovered to produce dosage association in females and in males. Our current data provide significant genetic evidence that P. ussuriensis has heterogametic XY sex chromosomes in males and homogametic XX sex chromosomes in females. Therefore, sex determination mechanism of P. ussuriensis is male heterogametic XX/XY system.

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“…As shown in Figure 1A, three cell lines all exhibited epithelial-like morphology. The three cell lines at passages of 10, 24 and 58 were cryopreserved and recovered successfully, respectively, according to methods previously described [27]. Briefly, after being subcultured for three days, the cells were trypsinized and suspended in 3 mL TC199 medium containing 10% dimethyl sulfoxide and 20% FBS.…”

Section: Introduction Methods and Resultsmentioning

confidence: 99%

Establishment of three cell lines from Chinese giant salamander and their sensitivities to the wild-type and recombinant ranavirus

Yuan

Chen

Huang

et al. 2015

Vet Res

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Known as lethal pathogens, Ranaviruses have been identified in diseased fish, amphibians (including Chinese giant salamander Andrias davidianus, the world’s largest amphibian) and reptiles, causing organ necrosis and systemic hemorrhage. Here, three Chinese giant salamander cell lines, thymus cell line (GSTC), spleen cell line (GSSC) and kidney cell line (GSKC) were initially established. Their sensitivities to ranaviruses, wild-type Andrias davidianus ranavirus (ADRV) and recombinant Rana grylio virus carrying EGFP gene (rRGV-EGFP) were tested. Temporal transcription pattern of ranavirus major capsid protein (MCP), fluorescence and electron microscopy observations showed that both the wild-type and recombinant ranavirus could replicate in the cell lines.

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Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses

Cited by 18 publications

References 37 publications

In vitro antiviral efficacy of moroxydine hydrochloride and ribavirin against grass carp reovirus and giant salamander iridovirus

In vitro antiviral efficacy of moroxydine hydrochloride and ribavirin against grass carp reovirus and giant salamander iridovirus

Identification of Sex-Specific Markers Reveals Male Heterogametic Sex Determination in Pseudobagrus ussuriensis

Establishment of three cell lines from Chinese giant salamander and their sensitivities to the wild-type and recombinant ranavirus

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