Ramalina capitata (Ach.) Nyl. acetone extract: HPLC analysis, genotoxicity, cholinesterase, antioxidant and antibacterial activity

Zrnzević, Ivana; Stanković, Miroslava; Stаnkov-Jovаnović, Vesnа; Mitić, Violeta; Đorđević, Aleksandra; Zlatanović, Ivana; Stojanović, Gordana

doi:10.17179/excli2017-301

Cited by 3 publications

(5 citation statements)

References 23 publications

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“…Our previous studies showed that the cupric-reducing capacity of Ramalina capitata, Umbilicaria crustulosa, Peltigera horizontalis, Hypogymnia tubulosa and Evernia prunastri acetone extracts were 6.11, 19.76, 9.94, 46.47 and 20.75 µg TE/mg dry extract, respectively. [34][35][36][37][38] Result obtained in the current study was very near to U. crustulosa and E. prunastri effect but much lower activity than H. tubulosa.…”

Section: Tpc and Antioxidant Activitysupporting

confidence: 55%

“…It seems that the optimal concentration of the extract for reducing the number of MN is 2.0 μg/mL, which is in agreement with the published results of other lichen species. [34][35][36][37][38] In this paper, for the first time, we report the antigenotoxic effect of acetone C. rangiformis extract.…”

Section: Cytokinesis-block Mn Assaymentioning

confidence: 87%

“…Higher concentrations of extracts and compounds under the study causes smaller decrease in the frequency of MN. [34][35][36][37][38] Therefore, the concentrations of 1.0, 2.0, and 3.0 μg/mL were chosen for this study.…”

Section: Cytokinesis-block Mn Assaymentioning

confidence: 99%

“…The measured antioxidant activity points to the conclusion that the capacity of the extract at a concentration of 15 mg/mL is significantly lower than the antioxidant capacity of the standards used at a concentration of 0.1 mg/mL, but in the range of the antioxidant capacity of extracts of other lichens. [34][35][36][37][38]…”

Section: Tpc and Antioxidant Activitymentioning

confidence: 99%

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Cladonia rangiformis Acetone Extract— New Insight into the Chemical Composition and Biological Activity

Dimitrijević,

Mitić,

Stankov Jovanović

et al. 2023

Natural Product Communications

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Introduction Cladonia rangiformis, also known as reindeer lichen, has been used for various remedies in folk medicine. The rich nutritional value of this lichen is reflected in the fact that it contains atranorin, which has been proven to have numerous biological activities. Objective The chemical composition, antioxidant, anticholinesterase, and antigenotoxic activities of the C. rangiformis acetone extract were investigated. The novelty of this work is that anticholinesterase activity, protective effect on human lymphocytes, and antioxidant activity by the cupric-reducing antioxidant capacity (CUPRAC) method of C. rangiformis extract were determined for the first time, as well as gas chromatography - mass spectrometry (GC-MS) profile. Methods Chemical composition was carried out by high-performance liquid chromatography (HPLC)-diode array detector and GC-MS. Antigenotoxic effect was evaluated in human lymphocytes by cytokinesis-block micronucleus assay. Total phenolic content, DPPH and ABTS radical scavenging capacities, CUPRAC, and total reducing power were determined spectrophotometrically. Determination of acetylcholinesterase activity was performed by Ellman's colorimetry assay. The statistical analysis of variance (One-way ANOVA) was performed using Origin software package version 7.0. Results HPLC method was used to identified 3 compounds with atranorin as predominant constituent (97.2%). Fumarprotocetraric and atraric acid were represented in a much smaller mutually approximate amount, 0.3 and 0.5%, respectively. Rangiformic acid was the most abundant volatile compound. Acetone extract of C. rangiformis (2 μg/mL) decreased the frequency of micronuclei by 15.5% while the radioprotectant amifostine reduced the frequency of occurrence of micronuclei by 11.4%. On the contrary, at a concentration of 1.0 mg/mL the extract exhibited an activating effect on cholinesterase (2.0%), while at a concentration of 10.0 mg/mL it showed a weak inhibitory effect (7.3%). The total phenol content was 132.71 μg gallic acid equivalents per mg of dry extract. The IC50 value for the DPPH experiment was 16.19 mg/mL and for ABTS was 11.80 mg/mL. Cupric reducing capacity and total reducing power were 17.81 μg Trolox equivalents per mg of dry extract and 0.45 μg ascorbic acid equivalents per mg of dry extract, respectively. Conclusion In addition to previously identified compounds in the acetone extract, atraric acid was identified for the first time by using HPLC method, and orcinol, β-orcinol, lauric alcohol, atranol, and rangiformic acid by using GC-MS method. The results of biological activity proved that acetone extract had antioxidant capacity and weak anticholinesterase activity. Reduction of the number of micronuclei in human lymphocytes, classified C. rangiformis as promising substances source with beneficial effects on DNA damage.

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Section: Tpc and Antioxidant Activitysupporting

confidence: 55%

Section: Cytokinesis-block Mn Assaymentioning

confidence: 87%

Section: Cytokinesis-block Mn Assaymentioning

confidence: 99%

Section: Tpc and Antioxidant Activitymentioning

confidence: 99%

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Cladonia rangiformis Acetone Extract— New Insight into the Chemical Composition and Biological Activity

Dimitrijević,

Mitić,

Stankov Jovanović

et al. 2023

Natural Product Communications

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“…The tallus lichens were separated from the stem, rinsed with running water, dried with tissue paper, and then weighed 5 mg each with a digital scale. The lichens were cut into small pieces and then soaked in 10 ml of 90% acetone (Zrnzević et al 2017) for 10 minutes. The extracts were filtered with filter paper and then added again with ethanol until reaching a size of 10 ml.…”

Section: Physiological Analysesmentioning

confidence: 99%

Short Communication: The effects of SO2 and NO2 fumigation on the chlorophyll of Parmotrema perlatum from Mt. Lawu, Cemoro Sewu, Indonesia

et al. 2023

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Abstract. Roziaty E, Sutarno, Suntoro, Sugiyarto. 2023. Short Communication: The effects of SO2 and NO2 fumigation on the chlorophyll of Parmotrema perlatum from Mt. Lawu, Cemoro Sewu, Indonesia. Biodiversitas 24: 2630-2637. Lichens are symbiotic organisms composed of algae and fungi. Lichens have long been recognized as bioindicators of environmental health. One of the lichen parts that will be affected by pollution is chlorophyll. The lichen thallus was fumigated with the gases from motor vehicle emissions. The lichens under study were Parmotrema perlatum (Huds.) M. Choisy, found in Cemoro Sewu Forest, Magetan, East Java. The categorization of vehicle emission exposure levels includes Level 0 (no fumigation, control group), Level 1 (1.5 hours), Level 2 (3 hours), Level 3 (4.5 hours), Level 4 (6 hours), Level 5 (7.5 hours) and Level 6 (9 hours). The fumigated thallus had already been examined with spectrophotometry. Sulfuric dioxide (SO2) was measured at 324 nm and 328 nm, whereas nitrogen dioxide and chlorophyll were measured at 645 nm and 663 nm. Each test was replicated three times (R1, R2, and R3). Level 6 had the highest NO2 and SO2 content. The highest NO2 content on thallus lichens was 4.00 at Level 6, while the lowest was 2.14 at Level 0 (Control). The highest SO2 content was 1.856 at Level 6, whereas the lowest was 0.231 at Level 0 (Control). The highest chlorophyll content of Parmotrema was found at Level 0, while the lowest was identified at Level 6 (0.245 µg ml-1). The highest content of chlorophyll b was 0.659 (Level 4), while the lowest was Level 6 (0.413 µg ml-1). The thallus Parmotrema responded positively to NO2 and SO2 exposure. Correlation tests between the four components, specifically fumigation, NO2, SO2, and chlorophyll, show a positive correlation between pollutants and chlorophyll.

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Optimizing an Enzymatic Extraction Method for the Flavonoids in Moringa (Moringa oleifera Lam.) Leaves Based on Experimental Designs Methodologies

et al. 2023

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Moringa oleifera Lam. is known to have significant antioxidant properties. Because of this, the development of an optimal extraction method is crucial to obtain pharmacological products based on the bioactive compounds produced by this tree. Through a Plackett–Burman and a Box–Behnken design, enzymatic extraction conditions (temperature, agitation, solvent pH and composition, sample-to-solvent ratio, enzyme-to-sample ratio and extraction time) have been optimized using normalized areas (UA/g) as response variable and relative mass (mg/g) as quantification variable. Extractions were performed in an incubator, where all the extraction conditions could be digitally controlled. Thus, 58.9 °C, 50 rpm, 4.0 pH, 32.5% EtOH, 0.2 g sample in 15 mL solvent and 106 U/g were established as the optimal extraction conditions for the extraction with a mix of pectinases coming from Aspergillus niger. Under these optimal conditions, two-minute extractions were performed and evaluated through a single factor design. The enzymatic extraction method demonstrated its suitability to produce extracts with good antioxidant power (antioxidant activity 4.664 ± 0.059 mg trolox equivalent/g sample and total phenolic compounds 6.245 ± 0.101 mg gallic acid equivalent/g sample). The method was also confirmed to have good repeatability (1.39%) and intermediate precision (2.37%) levels.

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Ramalina capitata (Ach.) Nyl. acetone extract: HPLC analysis, genotoxicity, cholinesterase, antioxidant and antibacterial activity

Cited by 3 publications

References 23 publications

Cladonia rangiformis Acetone Extract— New Insight into the Chemical Composition and Biological Activity

Cladonia rangiformis Acetone Extract— New Insight into the Chemical Composition and Biological Activity

Short Communication: The effects of SO2 and NO2 fumigation on the chlorophyll of Parmotrema perlatum from Mt. Lawu, Cemoro Sewu, Indonesia

Optimizing an Enzymatic Extraction Method for the Flavonoids in Moringa (Moringa oleifera Lam.) Leaves Based on Experimental Designs Methodologies

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