RAFT Polymers: Novel Precursors for Polymer—Protein Conjugates

Schilli, Christine; Müller, Axel H. E.; Rizzardo, Ezio; Thang, San H.; Chong, Y. K.

doi:10.1021/bk-2003-0854.ch041

Cited by 82 publications

(82 citation statements)

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“…A doubly hydrophilic block composed of acrylic acid and N-isopropylacrylamide (NIPAM) segments has also been prepared. [95] Other examples of doubly hydrophilic block copolymers have been reported by McCormick and coworkers. [244][245][246][247] In RAFT polymerization, the order of constructing the blocks of a block copolymer can be very important.…”

Section: Diblock Copolymersmentioning

confidence: 89%

“…Other applications of thiol functional polymers relate to the property of thiols to complex metals and to form conjugates with biological polymers, such as proteins. [95,196] …”

Section: End-functional Polymers and End-group Transformationsmentioning

confidence: 99%

“…[242] RAFT-synthesized thiols have also been used to make protein conjugates. [95,196] Summary RAFT polymerization has emerged as one of the most important methods for living radical polymerization. The method is robust and versatile and can be applied to the majority of polymers prepared by radical polymerization.…”

Section: Microgelsmentioning

confidence: 99%

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Living Radical Polymerization by the RAFT Process

Moad¹,

Rizzardo²,

Thang³

2005

Aust. J. Chem.

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2,134

1,894

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This paper presents a review of living radical polymerization achieved with thiocarbonylthio compounds [ZC( S)SR] by a mechanism of reversible addition-fragmentation chain transfer (RAFT). Since we first introduced the technique in 1998, the number of papers and patents on the RAFT process has increased exponentially as the technique has proved to be one of the most versatile for the provision of polymers of well defined architecture. The factors influencing the effectiveness of RAFT agents and outcome of RAFT polymerization are detailed. With this insight, guidelines are presented on how to conduct RAFT and choose RAFT agents to achieve particular structures. A survey is provided of the current scope and applications of the RAFT process in the synthesis of well defined homo-, gradient, diblock, triblock, and star polymers, as well as more complex architectures including microgels and polymer brushes.

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Section: Diblock Copolymersmentioning

confidence: 89%

“…Other applications of thiol functional polymers relate to the property of thiols to complex metals and to form conjugates with biological polymers, such as proteins. [95,196] …”

Section: End-functional Polymers and End-group Transformationsmentioning

confidence: 99%

See 1 more Smart Citation

Living Radical Polymerization by the RAFT Process

Moad¹,

Rizzardo²,

Thang³

2005

Aust. J. Chem.

Self Cite

2,134

1,894

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show abstract

“…First investigations of the RAFT polymerization of NHSMA with dithio benzoates and dithio carbamates only showed a moderate control and relatively broad molecular weight distributions (M w /M n [ 1.5). 39 In contrast, the copolymerization of NHSA with N,N-diethyl acrylamide was successful under RAFT conditions. 40 A good control of molecular weight (5000 g/mol \ M n \ 130,000 g/mol), narrow molecular weight distributions (M w /M n \ 1.1), and reasonable yields of up to 70% were achieved.…”

Section: Raft Polymerizationmentioning

confidence: 99%

Synthesis of well‐defined polymeric activated esters

Théato

2008

J. Polym. Sci. A Polym. Chem.

276

260

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Monomers bearing an activated ester group can be polymerized under various controlled polymerization techniques, such as ATRP, NMP, RAFT polymerization, or ROMP. Combining the functionalization of polymers via polymeric activated esters with these controlled polymerization techniques generate possibilities to realize highly functionalized polymer architectures. Within this highlight two different research areas of activated esters in polymer science will be discussed: (i) the preparation of defined reactive polymer architectures by controlled polymerization techniques and (ii) the preparation of defined reactive thin films. © 2008 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 6677–6687, 2008

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“…Part of the problem may have been the assay used to detect the -SH groups. [12][13][14] Antibodies were labeled with the DOTA-containing polymer tag 6 through free cysteine residues generated by partial reduction of the antibody, as depicted in Figure 1. The antibodies were reduced, washed in a centrifugal concentrator, and then a 10-fold excess of polymer tag was added, and the mixture was incubated at 37 °C for 1 hour.…”

mentioning

confidence: 99%

Polymer‐Based Elemental Tags for Sensitive Bioassays

et al. 2007

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To identify a rare (e.g., diseased or foreign) cell in a complex mixture, or to understand the proteomic complexity[1,2] of cells, one needs to be able to measure simultaneously and quantitatively a large number of proteins or other biomarkers that may be present in a complex sample. This is a difficult task and is beyond the reach of current capabilities. To address a problem of this complexity, we have begun to develop a high-sensitivity assay[3-6] based upon elemental tags that will enable the simultaneous measurement of many proteins in a single sample. The advantage of this approach lies in the large number of available elements and isotopes (potentially greater than 79) found in low abundance in biological systems, which will allow multiple tags to be used simultaneously. Inductively coupled plasma mass spectrometry (ICP-MS) is an ideal technique for detecting and quantifying these tags, as ICP-MS provides excellent resolution between the tag masses and an exceptional dynamic range (nine orders of magnitude). [7] This method allows one to overcome some of the limitations of currently available fluorescent tagging approaches.[8] These limitations arise from the spectral overlap of different dyes and the difficulty in measuring simultaneously targets that differ in abundance

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RAFT Polymers: Novel Precursors for Polymer—Protein Conjugates

Cited by 82 publications

References 0 publications

Living Radical Polymerization by the RAFT Process

Living Radical Polymerization by the RAFT Process

Synthesis of well‐defined polymeric activated esters

Polymer‐Based Elemental Tags for Sensitive Bioassays

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