Radiosynthesis of Gold/Albumin Core/shell Nanoparticles for Biomedical Applications

Abstract

Gold/albumin core/shell nanoparticles (Au/AlbNPs) was prepared by a novel aggregation/crosslinking technique and characterized by several spectroscopic and microscopy methods. Albumin, in presence of gold nanoparticles (AuNPs), is aggregated by the addition of ethanol and further stabilized by radiation-induced crosslinking using a 60Co source. Nanoconstructs are characterized to determine size, morphology and optical characteristics. The Au/AlbNPs were prepared in different ethanol and albumins concentrations. Results showed that it is possible to obtain Au/AlbNPs using ethanol 30 %v/v, albumin in different concentrations and an irradiation dose of 10 kGy. Au/AlbNP plasmon peak shifted to 530 nm, keeping the typical plasmon peak shape. The size of Au/AlbNPs is approximately double respect to the naked AuNPs and they show core/shell type morphology. The main amide peaks of albumin in FTIR spectrum can be found in the spectrum of nanoconstructs.

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“…ey can not only retain the physical and chemical properties of the central core and the shell materials but also endow the nanoparticles with some unique properties, such as biocompatibility, monodispersity, and high stability. is construction concept is widely utilized in preparing active SERS substrates [62]. For example, Akshaya et al [63] proposed a method of stepwise silver reduction on the surface of prefabricated gold nanoparticles to synthesize Au@Ag core-shell nanoparticles.…”

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“…Radiation-based synthesis for NPs construction can be carried out through degradation, crosslinking, graft copolymerization, and also radiation-induced reduction of metallic ions. This technique can be used for generating (i) radiation-crosslinked nanogels from synthetic/biopolymers [ 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ], (ii) conjugated NPs from size-controlled polymer templates through radiation [ 85 , 86 ], (iii) NPs from self-assembly of radiation-induced grafted copolymers [ 87 , 88 ], and (iv) AuNPs-hybrid NPs from radiation-induced reduction [ 89 , 90 , 91 , 92 , 93 , 94 ]. These are summarized in Table 3 .…”

“…Recently ready-to-use templates for radioactive 198 AuNPs, using biopolymers incorporating tumour-targeted peptides, have been successfully developed for use as nanomedicine agents in molecular imaging and therapy [ 91 ]. It has been proven that core-shell AuNPs can be efficiently synthesized in albumin protein solution under irradiation [ 92 , 93 , 95 ].…”