Radiolysis effect of the high proportion of ethanol in the preparation of albumin nanoparticle

Achilli, Estefanía Edith; Siri, Macarena; Flores, Constanza; Kikot, Pamela; Flor, Sabrina; Martinefski, Manuela; Lucangioli, Silvia; Alonso, Silvia del Valle; Grasselli, Mariano

doi:10.1016/j.radphyschem.2019.108387

Cited by 5 publications

(2 citation statements)

References 66 publications

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“…This means that in the case of reaction of • OH or

radicals with HSA, aggregates were formed that were not stabilized by DT bridges. The high reactivity of the • OH or radical

with amino acids contained within HSA is responsible for the formation of various covalent bonds (including C-C and S-S) between adjacent protein molecules [ 60 ]. Hashimoto et al explain the formation of DT in lysozyme solutions as a result of the reaction of this protein with

radical anion.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, a technological platform based on radiation cross-linked proteins and enzymes has been developed to obtain materials of biomedical importance. In our upcoming publications, we intend to address to important mechanistic aspects of synthesis by irradiation protein and enzymatic aggregates in line with the latest technological findings [ 6 , 60 , 71 , 72 , 73 , 74 ].…”

Section: Discussionmentioning

confidence: 99%

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Spontaneous and Ionizing Radiation-Induced Aggregation of Human Serum Albumin: Dityrosine as a Fluorescent Probe

Radomska

Wolszczak

2022

IJMS

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The use of spectroscopic techniques has shown that human serum albumin (HSA) undergoes reversible self-aggregation through protein–protein interactions. It ensures the subsequent overlapping of electron clouds along with the stiffening of the conformation of the interpenetrating network of amino acids of adjacent HSA molecules. The HSA oxidation process related to the transfer of one electron was investigated by pulse radiolysis and photochemical methods. It has been shown that the irradiation of HSA solutions under oxidative stress conditions results in the formation of stable protein aggregates. The HSA aggregates induced by ionizing radiation are characterized by specific fluorescence compared to the emission of non-irradiated solutions. We assume that HSA dimers are mainly responsible for the new emission. Dityrosine produced by the intermolecular recombination of protein tyrosine radicals as a result of radiolysis of an aqueous solution of the protein is the main cause of HSA aggregation by cross-linking. Analysis of the oxidation process of HSA confirmed that the reaction of mild oxidants (Br2•−, N3•, SO4•−) with albumin leads to the formation of covalent bonds between tyrosine residues. In the case of •OH radicals and partly, Cl2•−, species other than DT are formed. The light emission of this species is similar to the emission of self-associated HSA.

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“…This means that in the case of reaction of • OH or

radicals with HSA, aggregates were formed that were not stabilized by DT bridges. The high reactivity of the • OH or radical

radical anion.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Spontaneous and Ionizing Radiation-Induced Aggregation of Human Serum Albumin: Dityrosine as a Fluorescent Probe

Radomska

Wolszczak

2022

IJMS

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Polymer-based nanoparticles: fabrication to applications—the many faces of DC8,9PC and albumin

Grasselli

Alonso

2021

Biophys Rev

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This review is directed to researchers interested in a new point of view with relevance to nano-biomedicine. The first part covers the uses and potential of diacetylene lipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) in facilitating biological target recognition. The second part concentrates on the use of albumin as a "soft" coating for nanoparticles. This review makes comment on how fabricated nanoparticles will assist with future human health applications.

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Critical media attributes in E-beam sterilization of corneal tissue

Sharifi

Akbari

et al. 2022

Acta Biomaterialia

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Radiolysis effect of the high proportion of ethanol in the preparation of albumin nanoparticle

Cited by 5 publications

References 66 publications

Spontaneous and Ionizing Radiation-Induced Aggregation of Human Serum Albumin: Dityrosine as a Fluorescent Probe

Spontaneous and Ionizing Radiation-Induced Aggregation of Human Serum Albumin: Dityrosine as a Fluorescent Probe

Polymer-based nanoparticles: fabrication to applications—the many faces of DC8,9PC and albumin

Critical media attributes in E-beam sterilization of corneal tissue

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