Radioprotective effects of vitamin A against gamma radiation in mouse bone marrow cells

Changizi, Vahid; Haeri, Seyed Abolghasem; Abbasi, Saeed; Rajabi, Zahra; Mirdoraghi, Mohammad

doi:10.1016/j.mex.2019.03.020

Cited by 13 publications

(7 citation statements)

References 7 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With the understanding that free radicals perpetuate a significant amount of the damage caused by IR, vitamins with antioxidant potential (A, C, and E and its derivates) have been assayed as radioprotectors. Vitamin A and carotenes have antioxidant activity and capacity to enhance DNA repair, and in vivo reduced mortality and morbidity in mice exposed to partial or TBI [ 165 ]. Carotenoids such as crocin and crocetin (isolated from saffron) have antioxidant, anti-inflammatory and antiapoptotic effects [ 166 ].…”

Section: Medical Countermeasuresmentioning

confidence: 99%

Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry

et al. 2022

View full text Add to dashboard Cite

Atomic and radiological crises can be caused by accidents, military activities, terrorist assaults involving atomic installations, the explosion of nuclear devices, or the utilization of concealed radiation exposure devices. Direct damage is caused when radiation interacts directly with cellular components. Indirect effects are mainly caused by the generation of reactive oxygen species due to radiolysis of water molecules. Acute and persistent oxidative stress associates to radiation-induced biological damages. Biological impacts of atomic radiation exposure can be deterministic (in a period range a posteriori of the event and because of destructive tissue/organ harm) or stochastic (irregular, for example cell mutation related pathologies and heritable infections). Potential countermeasures according to a specific scenario require considering basic issues, e.g., the type of radiation, people directly affected and first responders, range of doses received and whether the exposure or contamination has affected the total body or is partial. This review focuses on available medical countermeasures (radioprotectors, radiomitigators, radionuclide scavengers), biodosimetry (biological and biophysical techniques that can be quantitatively correlated with the magnitude of the radiation dose received), and strategies to implement the response to an accidental radiation exposure. In the case of large-scale atomic or radiological events, the most ideal choice for triage, dose assessment and victim classification, is the utilization of global biodosimetry networks, in combination with the automation of strategies based on modular platforms.

show abstract

Section: Medical Countermeasuresmentioning

confidence: 99%

Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Dietmentioning

confidence: 99%

“…Charles River 3 , Taconic 4 , LabDiet 5 ) routinely make the composition of their diets publicly available; however, it is incumbent on researchers to ensure that the content of the feed will not impact the radiation exposure study. For example, many diets include known radiation injury protectants/mitigators, such as soy products ( Ohara et al 2001 ; Hillman et al 2011 ; Abernathy et al 2017 ; Landauer et al 2019 ), vitamin A ( Seifter et al 1988 ; Harapanhalli et al 1994 ; Roche et al 2015 ; Changizi et al 2019 ), vitamin C ( Narra et al 1994 ; Konopacka et al 1998 ; Kanter and Akpolat 2008 ; Satyamitra et al 2011 ; Mortazavi et al 2015 ; Roche et al 2015 ; Zangeneh et al 2015 ; Rostami et al 2016 ; Alexander et al 2018 ; Jafari et al 2018 ), vitamin D ( Langberg et al 2009 ; Gavrilov et al 2010 ; Marampon et al 2016 ) and vitamin E ( Ross et al 1983 ; Shaheen and Hassan 1991 ; Empey et al 1992 ; Nair et al 2003 ; Satyamitra et al 2003 ; Erol et al 2004 ; Singh et al 2013 ). In addition, certain estrogens ( Wang et al 2005 ; Fucic and Gamulin 2011 ), which have been shown to influence the degree of damage from radiation exposure, may also be found in rodent chow.…”

Section: Animal Husbandrymentioning

confidence: 99%

Study logistics that can impact medical countermeasure efficacy testing in mouse models of radiation injury

DiCarlo

Horta

Rios

et al. 2020

International Journal of Radiation Biology

View full text Add to dashboard Cite

Purpose: To address confounding issues that have been noted in planning and conducting studies to identify biomarkers of radiation injury, develop animal models to simulate these injuries, and test potential medical countermeasures to mitigate/treat damage caused by radiation exposure. Methods: The authors completed an intensive literature search to address several key areas that should be considered before embarking on studies to assess efficacy of medical countermeasure approaches in mouse models of radiation injury. These considerations include: (1) study variables; (2) animal selection criteria; (3) animal husbandry; (4) medical management; and (5) radiation attributes. Results: It is important to select mouse strains that are capable of responding to the selected radiation exposure (e.g. genetic predispositions might influence radiation sensitivity and proclivity to certain phenotypes of radiation injury), and that also react in a manner similar to humans. Gender, vendor, age, weight, and even seasonal variations are all important factors to consider. In addition, the housing and husbandry of the animals (i.e. feed, environment, handling, time of day of irradiation and animal restraint), as well as the medical management provided (e.g. use of acidified water, antibiotics, routes of administration of drugs, consideration of animal numbers, and euthanasia criteria) should all be addressed. Finally, the radiation exposure itself should be tightly controlled, by ensuring a full understanding and reporting of the radiation source, dose and dose rate, shielding and geometry of exposure, while also providing accurate dosimetry. It is important to understand how all the above factors contribute to the development of radiation dose response curves for a given animal facility with a well-defined murine model. Conclusions: Many potential confounders that could impact the outcomes of studies to assess efficacy of a medical countermeasure for radiation-induced injuries are addressed, and recommendations are made to assist investigators in carrying out research that is robust, reproducible, and accurate.

show abstract

“…El ensayo en roedores está incluido en la batería de estudios toxicológicos exigidos por la OECD. Es fácilmente reproducible, para ello se lava el fémur con suero fetal bovino, la médula se centrífuga a 800-1,000rpm/10 min; se elimina el sobrenadante y con el botón se realiza el frotis (32)(33)(34).…”

Section: Modelos Más Utilizados En La Prueba De Micronúcleosunclassified

Micronúcleos: Actualización del papel en la inestabilidad genética, inflamación, envejecimiento y cáncer. Revisión panorámica.

Bugarín

Arias-Ruiz

2023

revbiomed

View full text Add to dashboard Cite

La integridad del genoma es esencial para la salud y la continuidad de las especies. Los micronúcleos son biomarcadores de genotoxicidad, y protagonistas de inestabilidad y caos genético. Un micronúcleo o cuerpo de Howell-Jolly, contiene DNA independiente del núcleo, este se origina por retraso anafásico, cuya membrana es inestable y proclive a romperse sin reparación. El DNA contenido en estas estructuras puede condensarse, replicarse, dividirse, no obstante, de manera asincrónica al material genético nuclear, incluso puede sufrir de cromotripisis y reincorporarse al núcleo, lo cual provoca caos genético y con ello cáncer. Por otro lado, si el DNA del micronúcleo es liberado al citosol, desencadena la respuesta inflamatoria crónica, senescencia y apoptosis. Entonces, es vital detectar oportunamente a individuos susceptibles al daño genómico, lo cual permitiría priorizar la atención profiláctica. Por tanto, el objetivo fue hacer una revisión descriptiva sobre algunos de los modelos más empleados e innovadores en la prueba de micronúcleos y los avances celulares y moleculares, en el conocimiento de estas estructuras, así como sus efectos a la salud, para delinear una perspectiva y proponerlos como alternativa preventiva de fácil ejecución en la detección de individuos vulnerables a genotóxicos. Se buscaron artículos en MEDLIANE publicados en los años 2000-2023, que incluían los términos “micronuclei uses and causes” y “micronuclei causes and consecuences”. La inclusión final se determinó después de la revisión de los resúmenes y el posterior análisis del texto completo; se dejaron de incluir artículos una vez que fueron repetitivas las aportaciones.

show abstract

Radioprotective effects of vitamin A against gamma radiation in mouse bone marrow cells

Abstract: Graphical abstract

Cited by 13 publications

References 7 publications

Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry

Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry

Study logistics that can impact medical countermeasure efficacy testing in mouse models of radiation injury

Micronúcleos: Actualización del papel en la inestabilidad genética, inflamación, envejecimiento y cáncer. Revisión panorámica.

Contact Info

Product

Resources

About