Radioprotective effect of misoprostol on mouse 
spermatogonial stem cells

Rooij, Dirk G. de; Beek, M. E. A. B. van; Rutgers, Derk H.; Duyn-Goedhart, A. Van; Buul, Paul P.W. van

doi:10.1017/s0016672398003395

Cited by 6 publications

(3 citation statements)

References 14 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spermatogonial stem cells present within the seminiferous tubules differ morphologically from primordial germ cells (PGCs) and are called prospermatogonia (McLaren and Durcov‐Hills, 2001). In the adult testis, the first visible sign of differentiation of the stem cells is the formation of a pair of cells interconnected by an intercellular bridge (De Rooij et al, 1998).…”

mentioning

confidence: 99%

Long‐Term Culture and Transplantation of Murine Testicular Germ Cells

Jeong

McLean

Griswold

2003

Journal of Andrology

View full text Add to dashboard Cite

The objectives of this study were to develop an in vitro culture system to optimize germ cell proliferation and to measure the potential of the cultured germ cells to produce mature spermatozoa after transplantation into a recipient. Donor germ cells isolated from ROSA26 male mice were cultured with a STO feeder cell layer in Dulbecco's minimal essential medium (DMEM) supplemented with fetal bovine serum (FBS), stem cell factor, leukemia inhibitory factor, basic fibroblast growth factor, insulin-like growth factor 1, interleukin-11, L-glutamine, sodium pyruvate, 2-mercaptoethanol, murine oncostatin M, and platelet-derived growth factor. Donor germ cells formed colonies in the primary cultures after 8-21 days. These cultured colonies were maintained for 4 weeks or longer without subculture and proliferated for up to 8 passages over a period of 3 months. These colonies had alkaline phosphatase activity and incorporated 5-bromo-2'-deoxyuridine. These colonies were positive partially when screened with antibody for germ cell nuclear antigen and c-kit. Germ cells cultured with this supplemented medium showed enhanced colonization vs controls cultured with DMEM and FBS. Cultured germ cells from Rosa26 donors were transplanted into testes and were identified by X-gal staining and histological screening. The cells cultured in the supplemented medium colonized the tubules and initiated spermatogenesis in the recipient mice. This is an improved method for culturing germ cells and may be useful in gene therapy and the production of transgenic animals.

show abstract

mentioning

confidence: 99%

Long‐Term Culture and Transplantation of Murine Testicular Germ Cells

Jeong

McLean

Griswold

2003

Journal of Andrology

View full text Add to dashboard Cite

show abstract

“…Prostaglandin provides radioprotection to several tissues, including bone marrow and germinal epithelium . Misoprostol, a prostaglandin E1 analog, has been shown to have radioprotective efficacy …”

Section: Strategies To Develop Radioprotective Agentsmentioning

confidence: 99%

Radioprotective Agents: Strategies and Translational Advances

Kamran

Ranjan

Kaur

et al. 2016

Medicinal Research Reviews

104

View full text Add to dashboard Cite

Radioprotectors are agents required to protect biological system exposed to radiation, either naturally or through radiation leakage, and they protect normal cells from radiation injury in cancer patients undergoing radiotherapy. It is imperative to study radioprotectors and their mechanism of action comprehensively, looking at their potential therapeutic applications. This review intimately chronicles the rich intellectual, pharmacological story of natural and synthetic radioprotectors. A continuous effort is going on by researchers to develop clinically promising radioprotective agents. In this article, for the first time we have discussed the impact of radioprotectors on different signaling pathways in cells, which will create a basis for scientific community working in this area to develop novel molecules with better therapeutic efficacy. The bright future of exceptionally noncytotoxic derivatives of bisbenzimidazoles is also described as radiomodulators. Amifostine, an effective radioprotectant, has been approved by the FDA for limited clinical use. However, due to its adverse side effects, it is not routinely used clinically. Recently, CBLB502 and several analog of a peptide are under clinical trial and showed high success against radiotherapy in cancer. This article reviews the different types of radioprotective agents with emphasis on the strategies for the development of novel radioprotectors for drug development. In addition, direction for future strategies relevant to the development of radioprotectors is also addressed.

show abstract

“…Protected normal tissues; reduced frequencies of chromosomal aberrations hamster cells, mice and stem cell killing; protected against irradiation-induced oncogenic transformation of embryo cells and spermatogonial stem cells; protected both chromosome breakage and cell death in combination with vitamin E; selenomethionine and WR-3689 increased protective effect on normal tissue but not murine tumors. [179][180][181][182][183][184][185][186][187][188] Chemotherapy-induced hair loss, mice Protected hair follicles and increased hair survival. 189…”

Section: Irradiation Damagementioning

confidence: 99%

Misoprostol Therapeutics Revisited

2001

View full text Add to dashboard Cite

Misoprostol, a prostaglandin E1 analog, is a racemate of four stereoisomers. On administration it rapidly de-esterifies to its active form, misoprostolic acid. Misoprostolic acid is 85% albumin bound and has a half-life of approximately 30 minutes. It is excreted in urine as inactive metabolites. No significant drug interactions have been reported. Besides its gastrointestinal protective and uterotonic activities, misoprostol regulates various immunologic cascades. It inhibits platelet-activating factor and leukocyte adherence, and modulates adhesion molecule expression. It protects against gut irradiation injury, experimental gastric cancer, enteropathy, and constipation. It improves nutrient absorption in cystic fibrosis. Misoprostol has utility in acetaminophen and ethanol hepatotoxicity, hepatitis, and fibrosis. It is effective in asthmatics and aspirin-sensitive asthmatic and allergic patients. It lowers cholesterol and severity of peripheral vascular diseases, prolongs survival of cardiac and kidney transplantation, synergizes cyclosporine, and protects against cyclosporine-induced renal damage. It works against drug-induced renal damage, interstitial cystitis, lupus nephritis, and hepatorenal syndrome. It is useful in periodontal disease and dental repair. Misoprostol enhances glycosoaminoglycan synthesis in cartilage after injury. It prevents ultraviolet-induced cataracts and reduces intraocular pressure in glaucoma and ocular hypertension. It synergizes antiinflammatory and analgesic effects of diclofenac or colchicine and has been administered to treat trigeminal neuralgic pain. It reduces chemotherapy-induced hair loss and recovery time from burn injury, and is effective in treating sepsis, multiple sclerosis, and pancreatitis.

show abstract

Radioprotective effect of misoprostol on mouse spermatogonial stem cells

Cited by 6 publications

References 14 publications

Long‐Term Culture and Transplantation of Murine Testicular Germ Cells

Long‐Term Culture and Transplantation of Murine Testicular Germ Cells

Radioprotective Agents: Strategies and Translational Advances

Misoprostol Therapeutics Revisited

Contact Info

Product

Resources

About