Radioimmunoscintigraphy with melanoma-associated monoclonal antibody fragments in choroidal melanoma

Abstract

A prospective pilot study on radioimmunoscintigraphy with monoclonal antibody fragments against cutaneous melanoma (MoAb 225.28S) was carried out in 17 patients with a clinical diagnosis of choroidal melanoma. Monoclonal antibodies against melanoma-associated antigen were labeled with 740 mBq 99mTc and injected IV; images were made with a gamma camera at 6 h after injection. With a double-pinhole collimator, radioactivity was counted thrice in both eyes at 6 h after injection. In 6 of 16 patients (37.5%), the melanoma could be imaged with the gamma camera. With the double-pinhole collimator, a significantly higher activity was measured in the melanomatous eye in 13 of 16 patients (82.4%). In two patients a false negative result was obtained, and in one patient the difference between the left and right eye was not significant. Considering these results, radioimmunoscintigraphy may be valuable in ocular melanoma diagnostics, but the specificity of MoAb 225.28S needs to be assessed.

Clinical study of [123I] N‐(2‐diethylaminoethyl)‐4‐ iodobenzamide in the diagnosis of primary and metastatic ocular melanoma

“…BZA-scintigraphy showed that lesions of a size even below the resolution limit of the gamma camera can be visualized. 78% of the lesions with a thickness AE4 mm were imaged, versus 57% (same size) by IS (Schaling et al 1989). We also observed a trend toward positive scintigraphies with higher uptake when the tumor was strongly pigmented, but this was not statistically significant .…”

“…The first immunohistological studies showed the binding of MoAb 225-28 S raised against cutaneous melanoma in choroidal melanoma (Bomanji et al 1987;Scheiffarth et al 1989). Many clinical studies were performed to assess the value of immunoscintigraphy (I S) in choroidal melanoma (Schaling et al 1989(Schaling et al , 1994Hungerford et al 1991;Stanowsky et al 1993;Lö ffler et al 1994) and the largest clinical trial was published on 101 cases (Scheidler et al 1992). It appears to be a valuable method in the diagnosis of choroidal melanoma and offers substantial sensitivity and specificity in the differentiation of intraocular lesions.…”