Racemic, R-, and S-tebuconazole altered chitinase and chitobiase activity of Daphnia magna

Abstract

Tebuconazole is a chiral trizole fungicide and widely used in many crops for controlling disease. Tebuconazole is potential toxic to some aquatic organisms but relative information of its isomers is scarce. To detect the endocrine disrupting effects and difference of rac-, R-, and S-tebuconazole, the chitinase activity in Daphnia magna and chitobiase activity in each test medium were used as biomonitors after a 14-day exposure. Results showed that chitinase activity was significantly reduced by rac-, R-, and S-tebuconazole. The chitobiase activity in the test medium was reduced by rac- and R-tebuconazole before day 10, and only one peak was observed at day 10 or day 12 compared with two obvious peaks in the control group (days 6 and 12). S-tebuconazole delayed and reduced the reproduction of D. magna, but did not delay the first chitobiase activity peak, whereas the second peak could not be characterized as the exposure concentration and time increased. Compared with chitinase activity, chitobiase activity can still be used as a rudimentary model for identifying molt-interfering xenobiotics, and further studies should focus on the analysis of correlations between these parameters.

Persisting Effects in Daphnia magna Following an Acute Exposure to Flowback and Produced Waters from the Montney Formation

“…It is interesting to note that molting was not significantly decreased in any treatment; however, neonate production was decreased after 48 h FPW exposure (Figures and ), following a reduction in lipid metabolism (Figure ). Proteins involved in molting, such as chitinases 3 and 15, were found to increase in both FPW treatments (0.25 and 0.75%; Data File S1). Alternatively, probable chitinase 10 was decreased (Data File S1), which causes an excess in chitin production in Drosophila.…”

Efficacy of Allium sativum oil to alleviate tebuconazol-induced oxidative stress in the liver of adult rats

“…Tebuconazole is a very effective fungicide used for the control of mildew and rust on wheat, barly, rice, fruits and vegetables (2). It belongs to the group of triazole fungicides, whose mode of action is by the inhibition of the activity of lanosterol 14α-demethylase (CYP51), resulting in the membrane disruption and subsequent inhibition of cell growth (2,3). The disadvantages of fungicides include their toxicity to humans, animals, useful plants and the persistence (long life) of some of these chemicals in the environment (4).…”