Dietary Exposure to Tebuconazole Affects Testicular and Epididymal Histomorphometry in Frugivorous Bats

Machado‐Neves, Mariana; Neto, Mário J. O.; Miranda, Diane C.; Souza, Ana Cláudia Ferreira; Castro, Mariana Moraes de; Sertorio, Marcela Nascimento; Carvalho, Túlio F.; Matta, S. L. P.; Freitas, Mariella Bontempo

doi:10.1007/s00128-018-2377-6

Cited by 18 publications

(8 citation statements)

References 36 publications

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“…The correlation and PCA analysis have also indicated that tebuconazole presence may reduce longevity and egg viability in C. elegans. This fungicide toxicity in reproduction has already been reported in different animal models (Guimaraes et al 2018;Li et al 2020;Machado-Neves et al 2018;Fig. 5 Correlation analysis of all data, independent on samples collected pre-or post-pesticide application Fig.…”

Section: Discussionmentioning

confidence: 68%

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Kuhn

Jacques

Teixeira

et al. 2021

Environ Sci Pollut Res

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Uruguay River is the most important river in western Rio Grande do Sul, separating Brazil from Argentina and Uruguay. However, its pollution is of great concern due to agricultural activities in the region and the extensive use of pesticides. In a long term, this practice leads to environmental pollution, especially to the aquatic system. The objective of this study was to analyze the physicochemical characteristics, metals and pesticides levels in water samples obtained before and after the planting and pesticides' application season from three sites: Uruguay River and two minor affluents, Mezomo Dam and Salso Stream. For biomonitoring, the free-living nematode Caenorhabditis elegans was used, which were exposed for 24 h. We did not find any significant alteration in physicochemical parameters. In the pre-and post-pesticides' samples we observed a residual presence of three pesticides (tebuconazole, imazethapyr, and clomazone) and metals which levels were above the recommended (As, Hg, Fe, and Mn). Exposure to both pre-and post-pesticides' samples impaired C. elegans reproduction and post-pesticides samples reduced worms' survival rate and lifespan. PCA analysis indicated that the presence of metals and pesticides are important variables that impacted C. elegans biological endpoints. Our data demonstrates that Uruguay River and two affluents are contaminated independent whether before or after pesticides' application season. In addition, it reinforces the usefulness of biological indicators, since simple physicochemical analyses are not sufficient to attest water quality and ecological safety.

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Section: Discussionmentioning

confidence: 68%

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Kuhn

Jacques

Teixeira

et al. 2021

Environ Sci Pollut Res

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“…Toluidine blue is a metachromatic (a pH-dependent dye that stains cell elements a different color from the dye), staining heparin-containing granules purple or red [ 2 , 63 ]. As a simple, non-sensitive chemical, it can be applied to tissues after various methods of fixation and embedding [ 44 , 46 , 47 , 49 , 50 , 51 , 53 , 55 , 57 , 58 , 61 , 62 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 ]. However, its major disadvantage is the inability to distinguish immature from mature MCs, which could be done by alcian blue-safranin staining [ 45 , 48 , 67 , 72 , 93 ].…”

Section: Mast Cell Detection Methods In the Mammalian Testis And Epid...mentioning

confidence: 99%

Mast Cells in the Mammalian Testis and Epididymis—Animal Models and Detection Methods

Himelreich-Perić

Katušić-Bojanac

Hohšteter

et al. 2022

IJMS

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Mast cells (MCs) are an evolutionary well-conserved type of cells, mediating and modulating allergic responses in innate immunity and tissue remodeling after chronic inflammation. Among other tissues, they inhabit both the testis and epididymis. In the testis, MCs usually appear in the interstitial compartment in humans, but not in other standard experimental models, like rats and mice. MCs seem to be responsible for testicular tissue fibrosis in different causes of infertility. Although experimental animal models follow the effect on MC activation or penetration to the interstitial tissue like in humans to some extent, there is an inconsistency in the available literature regarding experimental design, animal strain, and detection methods used. This comprehensive review offers an insight into the literature on MCs in mammalian testes and epididymides. We aimed to find the most suitable model for research on MC and offer recommendations for future experimental designs. When using in vivo animal models, tunica albuginea incorporation and standard histological assessment need to be included. Domesticated boar strains kept in modified controlled conditions exhibit the highest similarity to the MC distribution in the human testis. 3D testicular models are promising but need further fine-tuning to become a valid model for MC investigation.

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“…This study coated papaya with the manufacturer's recommended application concentration of tebuconazole. When great fruit-eating bats (Artibeus lituratus) consumed the fruit, testicular and epididymal histomorphological changes were observed, suggesting that the fungicide tebuconazole may impact reproductive capacities in males [57]. Unfortunately, reproductive females were not evaluated.…”

Section: Reproductionmentioning

confidence: 99%

Pesticide Exposure Risks to Chiropteran Species and the Impacts on Emerging Zoonotic Diseases

Hooper¹,

Amelon²

2022

Bats - Disease-Prone but Beneficial

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Neonicotinoids have been in the spotlight in the pollinator community as they persist in the soil, have high water solubility, and have been associated with negative health implications on insect pollinators. The risk of new novel pesticides, including neonicotinoids, to bats are largely unknown. Bats have unique physiology as they are the only mammals capable of true and sustained flight, and have physiological adaptations including echolocation and torpor which under current protocols for acute and chronic toxicity studies in birds and terrestrial animals are not assessed. Due to these characteristics, some have argued that bats may serve as important bioindicators for ecosystem health and pesticide use. This chapter will focus on pesticides, and discuss the increased risk of exposure, morbidity, and mortality of bats species due to their unique physiology and natural life history. Special emphasis will be on potential increased risk of zoonotic disease transmission in bats exposed to emerging contaminants that suppress their immune system or cause increased biological stress.

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Dietary Exposure to Tebuconazole Affects Testicular and Epididymal Histomorphometry in Frugivorous Bats

Cited by 18 publications

References 36 publications

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Mast Cells in the Mammalian Testis and Epididymis—Animal Models and Detection Methods

Pesticide Exposure Risks to Chiropteran Species and the Impacts on Emerging Zoonotic Diseases

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