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El-Sayed, Mohamed; Kiani, Mohammad F.; Naimark, Mike D.; Hikal, Ahmed H.; Ghandehari, Hamidreza

doi:10.1023/a:1011066408283

Cited by 92 publications

(39 citation statements)

References 14 publications

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“…More recently, in vitro flow chambers have been used to study particulate drug carrier delivery to the endothelium via upregulation of adhesion molecules (Zou et al 2005; Sakhalkar et al 2003; Blackwell et al 2001; Dickerson et al 2001; El-Sayed et al 2001; Kiani et al 2002). A study using an idealized in vitro flow chamber (Patil et al 2001) investigated the adherence of 5-, 10-, 15-, and 20-μm diameter polystyrene microspheres with a PSGL-1 construct (Goetz et al 1997) to P-selectin.…”

Section: Introductionmentioning

confidence: 99%

Synthetic microvascular networks for quantitative analysis of particle adhesion

Prabhakarpandian

Pant

Scott

et al. 2008

Biomed Microdevices

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We have developed a methodology to study particle adhesion in the microvascular environment using microfluidic, image-derived microvascular networks on a chip accompanied by Computational Fluid Dynamics (CFD) analysis of fluid flow and particle adhesion. Microfluidic networks, obtained from digitization of in vivo microvascular topology were prototyped using soft-lithography techniques to obtain semicircular cross sectional microvascular networks in polydimethylsiloxane (PDMS). Dye perfusion studies indicated the presence of well-perfused as well as stagnant regions in a given network. Furthermore, microparticle adhesion to antibody coated networks was found to be spatially non-uniform as well. These findings were broadly corroborated in the CFD analyses. Detailed information on shear rates and particle fluxes in the entire network, obtained from the CFD models, were used to show global adhesion trends to be qualitatively consistent with current knowledge obtained using flow chambers. However, in comparison with a flow chamber, this method represents and incorporates elements of size and complex morphology of the microvasculature. Particle adhesion was found to be significantly localized near the bifurcations in comparison with the straight sections over the entire network, an effect not observable with flow chambers. In addition, the microvascular network chips are resource effective by providing data on particle adhesion over physiologically relevant shear range from even a single experiment. The microfluidic microvascular networks developed in this study can be readily used to gain fundamental insights into the processes leading to particle adhesion in the microvasculature.

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Section: Introductionmentioning

confidence: 99%

Synthetic microvascular networks for quantitative analysis of particle adhesion

Prabhakarpandian

Pant

Scott

et al. 2008

Biomed Microdevices

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“…PAMAM dendrimers were shown to interact with microvasculature and extravasate through endothelium because of their intrinsic charge. 23 The net surface charge of dendrimers is based on the nature of surface groups and the generation number of the dendrimers. The net surface charge of PAMAM dendrimers characterized by measuring their zeta potential was: 64.8 §3.2 mV for G7-NH 2 (amine surface), ¡42.0 §1.2 mV for G6.5-COOH (carboxyl dendrimers) and 27.7 §1 .1 mV for G7-OH (hydroxyl dendrimers).…”

Section: Pamam Dendrimersmentioning

confidence: 99%

Poly(amido amine) dendrimers in oral delivery

Yellepeddi

Ghandehari

2016

Tissue Barriers

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Poly(amidoamine) (PAMAM) dendrimers have been extensively investigated for oral delivery applications due to their ability to translocate across the gastrointestinal epithelium. In this Review, we highlight recent advances in the evaluation of PAMAM dendrimers as oral drug delivery carriers. Specifically, toxicity, mechanisms of transepithelial transport, models of the intestinal epithelial barrier including isolated human intestinal tissue model, detection of dendrimers, and surface modification are discussed. We also highlight evaluation of various PAMAM dendrimer-drug conjugates for their ability to transport across gastrointestinal epithelium for improved oral bioavailability. In addition, current challenges and future trends for clinical translation of PAMAM dendrimers as carriers for oral delivery are discussed.

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“…110 Dendrimer size has been exponentially correlated with the duration of extravasation across the endothelium, with larger dendrimers indicating faster extravasations. 111 The positive charges on the polyamine and/or polyamide linkages used in some dendrimers pose a potential risk for cellular toxicity and immune activation. Partial derivatization of the dendrimer surface with PEG and/or fatty acids is known to mitigate these concerns by shielding the positive charges on the surface.…”

Section: Structures Of Self-assembling Nanoparticlesmentioning

confidence: 99%

A review of solute encapsulating nanoparticles used as delivery systems with emphasis on branched amphipathic peptide capsules

Barros

Whitaker

Sukthankar

et al. 2016

Archives of Biochemistry and Biophysics

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Various strategies are being developed to improve delivery and increase the biological half-lives of pharmacological agents. To address these issues, drug delivery technologies rely on different nano-sized molecules including: lipid vesicles, viral capsids and nano-particles. Peptides are a constituent of many of these nanomaterials and overcome some limitations associated with lipid-based or viral delivery systems, such as tune-ability, stability, specificity, inflammation, and antigenicity. This review focuses on the evolution of bio-based drug delivery nanomaterials that self-assemble forming vesicles/capsules. While lipid vesicles are preeminent among the structures; peptide-based constructs are emerging, in particular peptide bilayer delimited capsules. The novel biomaterial— Branched Amphiphilic Peptide Capsules (BAPCs) display many desirable properties. These nano-spheres are comprised of two branched peptides— bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK, designed to mimic diacyl-phosphoglycerides in molecular architecture. They undergo supramolecular self-assembly and form solvent-filled, bilayer delineated capsules with sizes ranging from 20 nm to 2 microns depending on annealing temperatures and time. They are able to encapsulate different fluorescent dyes, therapeutic drugs, radionuclides and even small proteins. While sharing many properties with lipid vesicles, the BAPCs are much more robust. They have been analyzed for stability, size, cellular uptake and localization, intra-cellular retention and, bio-distribution both in culture and in vivo.

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Cited by 92 publications

References 14 publications

Synthetic microvascular networks for quantitative analysis of particle adhesion

Synthetic microvascular networks for quantitative analysis of particle adhesion

Poly(amido amine) dendrimers in oral delivery

A review of solute encapsulating nanoparticles used as delivery systems with emphasis on branched amphipathic peptide capsules

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