Quitosana fúngica sobre larvas de nematoides gastrintestinais de caprinos

Abstract

ABSTRACT:The goat is represented by a very considerable effective in the Northeastern Brazil, but infections caused by nematodes and the serious problem of parasitic resistance have become barriers to breed these animals. Alternatively, the control with bioproducts comes as a sustainable and viable solution to help breeding in this region. In this context, the present study evaluated the performance of fungal chitosan on the larval development of gastrointestinal nematodes in naturally infected goat samples. Therefore, the selection was performed at five properties. The positive herd was confirmed, and coprocultures were performed with chitosan solution 0.5, 1.0 and 1.5%, with each treatment performed in 5 replicates. The third-stage larvae (L3) were recovered and one hundred larvae/treatment were counted and identified. The identified genera were Haemonchus, Strongyloides, Oesophagostomum and Trichostrongylus. In the analysis of inhibition of larval development, the concentration of 1.0% prevented the development of larval Haemonchus by 35%, but the results were not statistically significant. Thus, it is suggested to seek new concentrations of fungal chitosan as anthelmintic, since it appears as a promising alternative to sustainable control of these endoparasites.KEYWORDS: larvicidal activity; natural anthelmintics; parasitic infection. RESUMO:A caprinocultura é representada por um efetivo bastante considerável no Nordeste brasileiro, porém, infecções causadas por nematoides e o sério problema da resistência parasitária se tornaram barreiras para a criação desses animais. Como alternativa, o controle com bioprodutos entra como uma solução sustentável e viável para auxiliar na criação da região. Nesse contexto, o presente trabalho avaliou a atuação da quitosana fúngica sobre o desenvolvimento larval de nematoides gastrintestinais em amostras de caprinos naturalmente infectados. Para tanto, foi realizada a seleção de 5 propriedades e confirmada a positividade do rebanho, além de coproculturas com solução de quitosana a 0,5; 1,0 e 1,5%, com cada tratamento realizado em 5 repetições. As larvas de terceiro estágio (L3) foram recuperadas e cem larvas por tratamento foram contabilizadas e identificadas. Os gêneros identificados foram Haemonchus, Strongyloides, Oesophagostomum e Trichostrongylus. Na análise da inibição do desenvolvimento larval, a concentração de 1,0% impediu o desenvolvimento larval do Haemonchus em 35%, porém, os resultados não tiveram diferença estatística significante. Assim, sugere-se buscar novas concentrações de quitosana fúngica como anti-helmíntico, visto que se apresenta como uma alternativa promissora no controle sustentável desses endoparasitos. PALAVRAS-CHAVE:atividade larvicida; anti-helmínticos naturais; infecção parasitária.

Preparation, Structural Characterization, and Property Investigation of Gallic Acid-Grafted Fungal Chitosan Conjugate

“…Fungal chitosan is already being produced and marketed by several companies in different countries, such as Belgium, Canada, and the United States, for medical and/or pharmaceutical applications [30]. Several studies have demonstrated the potential of fungal chitosan to act as an antifungal [31], antibacterial [32], antiparasitic [33,34], and anticancer agent [35]. However, despite the ecological advantages associated with its production as well as its non-allergenic nature, fungal chitosan is not used more widely than animal chitosan because of its lower yield.…”

Fungal chitosan as membranous material modified by atmospheric plasma

“…In recent years, some studies have shown the potential of fungal chitosan, such as antifungal, antibacterial, anticancer and antiparasitic action (Paiva et al, 2014;Souza Neto et al, 2017;Almutairi et al, 2020;Batista et al, 2020;Sathiyaseelan et al, 2020). For these reasons, fungal chitosan is already being produced and marketed by companies in different countries, such as Belgium, Canada and the United States.…”