Quercetin and curcumin in nanofibers of polycaprolactone and poly(hydroxybutyrate‐co‐hydroxyvalerate): Assessment of in vitro antioxidant activity

Abstract

Polymeric nanofibers are materials that can be used as scaffolds in tissue engineering. Quercetin and curcumin are antioxidants because of scavenge free radicals and chelate metal ions properties, protecting tissues of lipid peroxidation. The objective of this study was to develop a scaffold with potential antioxidant activity that was produced from nanofibers consisting of polycaprolactone (PCL) and a blend of PCL/poly(hydroxybutyrate-co-hydroxyvalerate) (PHB-HV) with the addition of quercetin or curcumin as the bioactive compound. Curcumin and quercetin were integrated into the solution at a concentration of 3%. The electrospun nanofibers were characterized using calorimetry and thermogravimetric analysis, and the addition of bioactive compounds did not alter the thermal properties of the biomaterial. The antioxidant activity of scaffolds with the active compounds was evaluated by hydrate 2,2-diphenyl-2-picrylhydrazyl (DPPH) and 2,2 0 -azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) methods. The scaffolds with PCL and PCL/PHB-HV blend with quercetin exhibited higher antioxidant activity than curcumin with both methods.

Fabrication of nanofiber coated with l-arginine via electrospinning technique: a novel nanomatrix to counter oxidative stress under crosstalk of co-cultured fibroblasts and satellite cells

“…These results of our scavenging radical activity were consistent with a previous report showing that PCL nanofiber conjugated with quercetin had the highest antioxidant activity in DPPH and ABTS assays (da Silva Uebel et al 2016). Inhibition between 25% and 50% is considered as median range while DPPH inhibition of more than 75% is considered as having the best antioxidant activity.…”

“…Inhibition between 25% and 50% is considered as median range while DPPH inhibition of more than 75% is considered as having the best antioxidant activity. The quercetin coated with poly(Ecaprolactone) scaffold and polymeric nanoparticles respectively exhibited free radical scavenging activity against DPPH and could prevent the tissue damage and accelerating the healing process (da Silva Uebel et al 2016). Hence, PU nanofiber containing L-Arginine released rapidly which may reduce adverse effects found in damaged tissues by removing products of radicals and reduce oxidative stress.…”

“…Radical scavenging activity of nanofiber conjugated with L-arginine was carried out using published method by da Silva Uebel et al (2016). Briefly, 5, 10, 20, and 40 mg of dried conjugated nanofibers were dipped into 5 ml of DMSO solution in a test-tube and then kept in an ultrasonic bath (UIL-Ultrasonic Co. Ltd., South Korea) for 10 min for dispersion of mixed amino acids.…”

Influence of flavonoids on mechanism of modulation of insulin secretion

“…[ 70 ] However, the oral bioavailability of quercetin is poor which hinders wide applications,[ 71 ] which are directed to the determination of improved bioavailability from the incorporation in nanomaterials. [ 72 73 ]…”

Applications of Electrospun Nanofibers with Antioxidant Properties: A Review

“…The release profile of phycocyanin also showed similarities between 3% and 6% spirulina concentrations, which could have been for the same reason. Quercetin (Que) and curcumin (Cur) were individually loaded onto PCL and blended with poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) by Da Silva et al [52]: they intended to use the scavenging capacity of free radicals and chelate metal ions to develop a scaffold to stimulate tissue recovery. PHBV is a copolymer of bacterial origin belonging to the polyhydroxyalkanoate (PHA) group.…”