Fabrication of nanofiber coated with <scp>l</scp>-arginine via electrospinning technique: a novel nanomatrix to counter oxidative stress under crosstalk of co-cultured fibroblasts and satellite cells

Subramaniyan, Sivakumar Allur; Sheet, Sunirmal; Saravanakumar, B.; Vetha, Berwin Singh Swami; Rampa, Dileep Reddy; Shanmugam, Subbiah; Kang, Darae; Choe, Ho Sung; Shim, Kwan Seob

doi:10.1080/15419061.2018.1493107

Cited by 11 publications

(5 citation statements)

References 35 publications

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“…Polymers such as polyvinyl alcohol (PVA) [30,79], PCL [80] and PEO [44,76,81,82] are required to achieve a bead-free morphology and overcome brittleness of the material (Table 1). Only a limited number of studies have, therefore, focused on applying soy-based nanofibers for wound healing [44,[81][82][83], even though soy protein contains reactive amino acid residues such as arginine, glycine, aspartic acid and glutamine that facilitate wound healing through cell attachment and proliferation [83][84][85][86]. Higher cell proliferation in vitro has been demonstrated in electrospun soy protein in comparison to a solvent cast film, which has been attributed to the porous nanofibrous matrix that allows better nutrient access [83].…”

Section: Soy Proteinmentioning

confidence: 99%

Electrospinning Proteins for Wound Healing Purposes: Opportunities and Challenges

Akhmetova

Heinz

2020

Pharmaceutics

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With the growth of the aging population worldwide, chronic wounds represent an increasing burden to healthcare systems. Wound healing is complex and not only affected by the patient’s physiological conditions, but also by bacterial infections and inflammation, which delay wound closure and re-epithelialization. In recent years, there has been a growing interest for electrospun polymeric wound dressings with fiber diameters in the nano- and micrometer range. Such wound dressings display a number of properties, which support and accelerate wound healing. For instance, they provide physical and mechanical protection, exhibit a high surface area, allow gas exchange, are cytocompatible and biodegradable, resemble the structure of the native extracellular matrix, and deliver antibacterial agents locally into the wound. This review paper gives an overview on cytocompatible and biodegradable fibrous wound dressings obtained by electrospinning proteins and peptides of animal and plant origin in recent years. Focus is placed on the requirements for the fabrication of such drug delivery systems by electrospinning as well as their wound healing properties and therapeutic potential. Moreover, the incorporation of antimicrobial agents into the fibers or their attachment onto the fiber surface as well as their antimicrobial activity are discussed.

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Section: Soy Proteinmentioning

confidence: 99%

Electrospinning Proteins for Wound Healing Purposes: Opportunities and Challenges

Akhmetova

Heinz

2020

Pharmaceutics

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“…However, despite the fact that arginine plays an important role in the process of tissue regeneration, few examples of its use in the modification of polymer scaffolds exist. It has been found that electrospun polyurethane modified with arginine counters oxidative stress in vitro, thus increasing its utility as a wound dressing [15]. Arginine has been used to modify PCL by electrospinning in the formation of scaffolds [16], and it was found that increasing the arginine concentration in the spinning solution decreased the diameter of the formed fibers.…”

Section: Introductionmentioning

confidence: 99%

Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell Cultivation

et al. 2020

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This article describes the modification conditions and properties of polymer films obtained using a solution of poly(ε-caprolactone) modified with arginine. We investigated the effects on the surface and biological properties of films created using various arginine concentrations and temperature conditions during the modification process. We found that both increasing the arginine concentration of the treatment solution or the temperature of the treatment reaction increased the arginine content of the film. Following a cellular cultivation period of 3 days, greater levels of cell proliferation were observed on all modified poly(ε-caprolactone) films compared to unmodified polymer films. Experiments using fluorescence microscopy showed that the modification conditions also had a significant effect on cellular spreading and the organization of the actin cytoskeleton following 2 h of cultivation. The degree of spreading and actin cytoskeleton organization observed in cells on these modified polymer films was superior to that of the control films.

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“…Modifying nanofibers with L-arginine has been dealt with in only a small number of papers. Subramaniyan et al coated polyurethane nanofibers with L-arginine for biological in vitro testing [20]. Other authors have modified the surface of a lignin or cellulose nanofiber gel with L-arginine for wound healing [21,22].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo testing of nanofibrous membranes doped with alaptide and L-arginine for wound treatment

et al. 2020

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We have prepared a candidate biocompatible construct for skin wound healing based on electrospun polycaprolactone (PCL) nanofibrous membranes. The membrane material was loaded either with L-arginine or with alaptide, or with a mixture of both bioactive components. Alaptide is a spirocyclic synthetic dipeptide, an analogue of melanocyte-stimulating hormone release-inhibiting factor. L-arginine is an amino acid with a basic guanidine side chain. It is a direct precursor of nitric oxide, which plays a pivotal role in skin repair. The presence and the distribution of the additives were proved with high-performance liquid chromatography, Fourier-transform infrared spectroscopy and Raman spectroscopy. The influence of L-arginine and alaptide on the morphology of the membrane was characterized using scanning electron microscopy. No statistically significant correlation between fiber diameter and drug concentration was observed. The membranes were then tested in vitro for their cytotoxicity, using primary human dermal fibroblasts, in order to obtain the optimal concentrations of the additives for in vivo tests in a rat model. The membranes with the highest concentration of L-arginine (10 wt. %) proved to be cytotoxic. The membranes with alaptide in concentrations from 0.1 to 2.5 wt.%, and with the other L-arginine concentrations (1 and 5 wt.%), did not show high toxicity. In addition, there was no observed improvement in cell proliferation on the membranes. The in vivo experiments revealed that membranes with 1.5 wt.% of alaptide or with 1.5 wt.% of alaptide in combination with 5 wt.% of L-arginine markedly accelerated the healing of skin incisions, and particularly the healing of skin burns, i.e. wounds of relatively large extent. These results indicate that our newly-developed nanofibrous membranes are promising for treating wounds with large damaged areas, where a supporting material is needed.

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Fabrication of nanofiber coated with l-arginine via electrospinning technique: a novel nanomatrix to counter oxidative stress under crosstalk of co-cultured fibroblasts and satellite cells

Cited by 11 publications

References 35 publications

Electrospinning Proteins for Wound Healing Purposes: Opportunities and Challenges

Electrospinning Proteins for Wound Healing Purposes: Opportunities and Challenges

Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell Cultivation

In vitro and in vivo testing of nanofibrous membranes doped with alaptide and L-arginine for wound treatment

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