Quantitative Thin Layer Chromatographic Multi-Sulf onamide Screening Procedure

Abstract

In-situ optical scanning of f luorescamine derivatives on thin layer silica gel plates provides a rapid method for the determination of multiple sulfonamides at levels below 0.1 ppm. Sample preparation is minimal. Homogenized liver or muscle is extracted with ethyl acetate and then back-extracted into 0.2M glycine buffer. After pH adjustment, the extract is washed with hexane and extracted with methylene chloride. The organic phase is evaporated to dryness and reconstituted in methanol. Pre-adsorbent layer silica gel plates are used for chromatography. The method has been applied to residues of sulfamethazine, sulfadimethoxine, sulfathiazole, sulfaquinoxaline, and sulfabromomethazine in cattle, swine, turkey, and duck tissues.

Development and validation (according to the 2002/657/EC regulation) of a method to quantify sulfonamides in porcine liver by fast partition at very low temperature and LC-MS/MS

“…5 Enzyme linked immune sorbent assay (ELISA), although being a non-specific method, has been largely used. 6 Other analytical methods have been used, such as thin-layer chromatography (TLC), 7 high-performance liquid chromatography (HPLC), 8,9 high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS), [10][11][12] and capillary electrophoresis-mass spectrometry. 13 Hence, whereas ELISA is the most typical method for a fast screening and batch analysis, HPLC-MS/MS has been widely used due to its high sensitivity and broad linear range and to its capability to supply accurate and precise quantifications.…”