Quantitative spectroscopy analysis of prokaryotic cells: vegetative cells and spores

Alupoaei, Catalina E.; Olivares, José A.; García‐Rubio, Luis H.

doi:10.1016/j.bios.2003.08.021

Cited by 51 publications

(50 citation statements)

References 15 publications

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“…2(a)) and B. subtilis (Fig. 2(b)), most likely because the size and water content of these bacteria are similar (Ross and Billing, 1957;Alupoaei et al, 2004). Moreover, the morphology and membrane surface roughness of both bacteria are similar to each other Nikiyan et al, 2010), which would be one of the reasons for similar spectral absorption behavior of both bacteria.…”

Section: Application To Bioaerosolsmentioning

confidence: 83%

“…Alupoaei et al (2004) reported that spectra of bacteria are divided into spectra of macrostructure and spectra of internal structure, and suggested the following equation that expresses turbidity τ(λ) measured at a given wavelength λ:…”

Section: Structure Of Bacterial Cellsmentioning

confidence: 99%

“…Operation of a traditional flow cytometer still relies on the need for specialized personnel and complicated sample pretreatment procedures. Ultraviolet and visible (UV-Vis) spectroscopy, which measures the amount of light that is attenuated by a solution or a suspension of particles, is a quantitative, reliable, and rapid analytical tool that can be immediately applied as a biosensor for the detection, identification, and enumeration of cells (An and Friedman, 1997;Alupoaei et al, 2004;Alupoaei and García-Rubio, 2005). UV-Vis spectroscopy of cells has been used to estimate the number of cells in a solution and their chemical composition, including nucleic acid and protein concentrations (Leštan et al, 1993;Wang et al, 1995;Piereira et al, 2000;Alupoaei, 2001;Salard et al, 2001;Alupoaei and García-Rubio, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

Park

Yoon

Byeon³

et al. 2012

Aerosol Air Qual. Res.

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We evaluated a method for the assessment of bacterial viability that is based on ultraviolet and visible (UV-Vis) spectroscopy analysis. The quantities of intracellular materials inside a cell vary depending on change of bacterial viability by disruption of the membrane integrity. Therefore, normalized optical density in the range of 200-290 nm was analyzed to determine if it varied in samples containing different proportions of live bacteria. Our results indicate that samples containing higher proportions of live bacteria such as Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis had higher optical densities. In addition, the optical density at 230 nm divided by the optical density at 670 nm was found to have a strong linear correlation with bacterial viability (the R 2 values of E. coli, B. subtilis, and S. epidermidis are 0.9964, 0.9118, and 0.9861, respectively). Our proposed rapid assessment method takes less than three minutes and only requires optical measurements at 230 and 670 nm; therefore, it is simpler and faster than colony counting, fluorochromasia, or the dyeexclusion test. Moreover, our method was applied to bioaerosols, which are currently important issues in public health, microbiology, aerosol science, and other fields. In our study, the bacteria (E. coli) were dispersed into the air using a Collisontype atomizer, and were sampled in sterilized deionized water using an impinger with a pump. According to our method, the viability of E. coli was approximately 55.2%, which was similar to 52.5 ± 4.7% determined from the LIVE/DEAD BacLight bacterial viability assay.

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Section: Application To Bioaerosolsmentioning

confidence: 83%

Section: Structure Of Bacterial Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

Park

Yoon

Byeon³

et al. 2012

Aerosol Air Qual. Res.

View full text Add to dashboard Cite

show abstract

“…A multiwavelength, UV/visible spectroscopic method of detecting pathogens that uses both the light-scattering and absorbing properties of vegetative cells and spores to generate a spectrum is under development (7,8). It incorporates a relatively simple model to interpret the resulting spectrum.…”

Section: Chemical and Physical Detectionmentioning

confidence: 99%

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

et al. 2005

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SUMMARY Recent events have made public health officials acutely aware of the importance of rapidly and accurately detecting acts of bioterrorism. Because bioterrorism is difficult to predict or prevent, reliable platforms to rapidly detect and identify biothreat agents are important to minimize the spread of these agents and to protect the public health. These platforms must not only be sensitive and specific, but must also be able to accurately detect a variety of pathogens, including modified or previously uncharacterized agents, directly from complex sample matrices. Various commercial tests utilizing biochemical, immunological, nucleic acid, and bioluminescence procedures are currently available to identify biological threat agents. Newer tests have also been developed to identify such agents using aptamers, biochips, evanescent wave biosensors, cantilevers, living cells, and other innovative technologies. This review describes these current and developing technologies and considers challenges to rapid, accurate detection of biothreat agents. Although there is no ideal platform, many of these technologies have proved invaluable for the detection and identification of biothreat agents.

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“…refractive index dispersion, can be determined. The application of the scattering spectroscopy technique to refractometry of cells, bacteria, and spores has been also reported [4,14,15,16].…”

Section: Introductionmentioning

confidence: 97%

Refractometry of organosilica microspheres

et al. 2007

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The refractive index of novel organosilica (nano/micro)material is determined using two methods. The first method is based on analysis of optical extinction efficiency of organosilica beads versus wavelength, which is obtained by a standard laboratory spectrometer. The second method relies on the measurable trapping potential of these beads in the focused light beam (laser tweezers). Polystyrene beads were used to test these methods, and the determined dispersion curves of refractive index values have been found accurate. The refractive index of organosilica beads has been determined to range from 1.60-1.51 over the wavelength range of 300-1100 nm.

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Quantitative spectroscopy analysis of prokaryotic cells: vegetative cells and spores

Cited by 51 publications

References 15 publications

Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

Refractometry of organosilica microspheres

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